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Changes in the calcium metabolism of Biomphalaria glabrata experimentally infected with Angiostrongylus cantonensis

Published online by Cambridge University Press:  07 January 2013

V.M. Tunholi-Alves ,
V.M. Tunholi ,
J. Silva Garcia ,
S.F. Costa-Neto ,
A. Maldonado Jr ,
M.A.J. Santos ,
S.C. Thiengo  and
J. Pinheiro
V.M. Tunholi-Alves
Affiliation:
Curso de Pós-Graduação em Ciências Veterinárias, Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Km7, BR 465, Antiga estrada Rio-São Paulo, 23890-000Seropédica, RJ, Brazil Departamento de Ciências Fisiológicas, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Km7, BR 465, Antiga estrada Rio-São Paulo, 23890-000Seropédica, RJ, Brazil
V.M. Tunholi
Affiliation:
Curso de Pós-Graduação em Ciências Veterinárias, Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Km7, BR 465, Antiga estrada Rio-São Paulo, 23890-000Seropédica, RJ, Brazil Departamento de Ciências Fisiológicas, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Km7, BR 465, Antiga estrada Rio-São Paulo, 23890-000Seropédica, RJ, Brazil
J. Silva Garcia
Affiliation:
Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz, Fiocruz, Av. Brasil, 4365-Manguinhos, 21040-900Rio de Janeiro, RJ, Brazil
S.F. Costa-Neto
Affiliation:
Curso de Pós-Graduação em Ciências Veterinárias, Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Km7, BR 465, Antiga estrada Rio-São Paulo, 23890-000Seropédica, RJ, Brazil
A. Maldonado Jr
Affiliation:
Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz, Fiocruz, Av. Brasil, 4365-Manguinhos, 21040-900Rio de Janeiro, RJ, Brazil
M.A.J. Santos
Affiliation:
Departamento de Biologia Animal, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Km7, BR 465, Antiga estrada Rio-São Paulo, 23890-000Seropédica, RJ, Brazil
S.C. Thiengo
Affiliation:
Laboratório de Referência Nacional em Malacologia Médica do Instituto Oswaldo Cruz, IOC, Fiocruz, Av. Brasil, 4365-Manguinhos, 21040-900Rio de Janeiro, RJ, Brazil
J. Pinheiro*
Affiliation:
Curso de Pós-Graduação em Ciências Veterinárias, Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Km7, BR 465, Antiga estrada Rio-São Paulo, 23890-000Seropédica, RJ, Brazil Departamento de Ciências Fisiológicas, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Km7, BR 465, Antiga estrada Rio-São Paulo, 23890-000Seropédica, RJ, Brazil
*
* E-mail: [email protected]
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Abstract

Levels of calcium in the haemolymph and reserves in the shell of Biomphalaria glabrata experimentally infected by Angiostrongylus cantonensis were determined for the first time. At the same time, histochemical analyses of the digestive gland of infected and uninfected snails were performed to better understand the possible changes in metabolism of calcium in these organisms. After 1, 2 and 3 weeks of infection, the snails were dissected for collection of haemolymph and separation of tissues. The highest calcium concentrations in the haemolymph were found 2 weeks after infection, with a 39.61% increase in relation to the respective control group. However, there was a significant reduction in the concentration of this ion in the haemolymph of infected snails after 1 week of infection in relation to the uninfected specimens. In parallel, intense hypocalcification was shown in the shell of infected snails 1 and 2 weeks after infection, differing significantly in relation to the respective control groups. Morphological changes in the digestive gland of infected snails were also observed, confirming the role of this ion as an important element in the parasite encapsulation process.

Type
Research Papers
Information
Journal of Helminthology , Volume 88 , Issue 2 , June 2014 , pp. 160 - 165
Copyright
Copyright © Cambridge University Press 2013 

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