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Cellular immune response studies in bancroftian filariasis

Published online by Cambridge University Press:  05 June 2009

J. Regunathan ,
K. Jayaraman  and
P. Kaliraj
J. Regunathan
Affiliation:
Centre for Biotechnology, Anna University, Madras 600 025, India
K. Jayaraman
Affiliation:
Centre for Biotechnology, Anna University, Madras 600 025, India
P. Kaliraj*
Affiliation:
Centre for Biotechnology, Anna University, Madras 600 025, India
*
*Author for correspondence.
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Abstract

An attempt was made to identify the filarial specific antigens that are capable of inducing immune response in human filariasis. Lymphocytes were taken from three clinically defined groups living in an endemic area in Madras, namely microfilaraemic (MF) subjects with microfilariae in their blood smear without any clinical symptoms, chronic pathology (CP) individuals with lymphangitis or lymphadenitis in combination with a history of recurrent filarial fevers or lymphoedema, and endemic normals (EN) subjects without microfilariae nor any clinical symptoms of pathology. Lymphocytes from the three groups responded with no significant difference (P = 0.21) in their proliferative index to PPD and PHA, although lymphocytes from MF individuals showed significantly (P < 0.001) less proliferative index to Brugia malayi antigen (BMA) than the CP and EN subjects. This antigen specific cellular unresponsiveness seen in MF patients was not reversed by the addition of recombinant IL-lα, IL-1β, and IFN-γ, but the addition of sera from EN individuals seemed to restore this unresponsiveness (P < 0.001). The peripheral blood mononuclear cells from MF patients secreted more IL-1 in response to BMA induction than the same from CP and EN individuals. A 58 kDa recombinant protein isolated from a Wuchereria bancrofti genomic library (58 kDa) had mounted a higher proliferative response to lymphocytes from all three groups compared to BMA (P < 0.001) indicating the possible use of recombinant filarial protein to mount immunological responses in filarial patients.

Type
Research Note
Information
Journal of Helminthology , Volume 71 , Issue 3 , September 1997 , pp. 265 - 267
Copyright
Copyright © Cambridge University Press 1997

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References

Abou-Zeid, C., Filley, E., Steele, J. & Rook, G.A.W. (1987) A simple new method for using antigens separated by PAGE to stimulate lymphocytes in vitro after converting bands cut from Western blots into antigen bearing particles. Journal of Immunological Methods 98, 5–10.CrossRefGoogle Scholar
Faist, E.A., Baker, T., Strasses, S.S., Reiber, A.P. & Heberer, G. (1987) Prostaglandin dependent suppression of IL-2 production in patients with major trauma. Journal of Trauma 27, 837–840.CrossRefGoogle ScholarPubMed
Harding, A.F., McArthur, G.J., Cross, A.J., Raulet, H.D. & Allison, J.P. (1992) CD-28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature 356, 607–609.CrossRefGoogle Scholar
Hochuli, E., Bannworth, W., Dobeli, H., Gentz, R. & Stuber, D. (1988) Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Biotechnology 1, 1321–1325.Google Scholar
Jenkins, M.K., Pardoll, D.M., Mizuguchi, J., Chused, T.M. & Schwartz, R.H. (1987) Molecular events in the induction of a non-responsive state in interleukin 2-producing helper T-lymphocyte clones. Proceedings of the National Academy of Sciences, USA 84, 5409–5412.CrossRefGoogle Scholar
Knudsen, P., Dinarello, C.A. & Storm, T. (1986) Prostaglandins post-transcriptionally inhibit monocyte expression of IL-1 activity by increasing intracellular cyclic adenosine monophosphate. Journal of Immunology 137, 3189–3194.CrossRefGoogle Scholar
Lichtman, A.H., Chin, J., Schmidt, J.A. & Abbas, A.K. (1988) Role of IL-1 in the activation of T-lymphocytes Proceedings of the National Academy of Sciences, USA 85, 9699–9703.CrossRefGoogle ScholarPubMed
Raghavan, N., McReynolds, L.A., Maina, C.V., Feinstone, S.M., Jayarman, K., Ottesen, E.A. & Nutman, T.B. (1991) A recombinant clone of Wuchereria bancrofti with DNA specificity for human lymphatic filarial parasites. Molecular and Biochemical Parasitology 47, 63–72.CrossRefGoogle ScholarPubMed
Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Plasmid vectors. pp. 1.2–1.105 in Molecular cloning, a laboratory manual. Cold Spring Harbor, New York, Cold Spring Harbor Laboratory Press.Google Scholar
Theodore, J.G., Kaliraj, P., Jayachandran, S. & Jayaraman, K. (1993) Cloning over expression and evaluation of a recombinant fusion protein of Wuchereria bancrofti towards its application as a diagnostic agent for bancroftian filariasis. Parasitology 106, 413–420.CrossRefGoogle ScholarPubMed