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Biodiversity of entomopathogenic nematodes in Italy

Published online by Cambridge University Press:  10 April 2014

E. Tarasco ,
M. Clausi ,
G. Rappazzo ,
T. Panzavolta ,
G. Curto ,
R. Sorino ,
M. Oreste ,
A. Longo ,
D. Leone  and
R. Tiberi
...Show all authors
E. Tarasco
Affiliation:
Dipartimento di Scienze del Suolo della Pianta e degli Alimenti (Di.S.S.P.A.), Università degli Studi di Bari ‘Aldo Moro’, Via Amendola 165/A, 70126Bari, Italy
M. Clausi*
Affiliation:
Dipartimento di Scienze Biologiche, Geologiche e Ambientali, Sez. Biologia Animale ‘M. La Greca’, Università degli Studi di Catania, Via Androne 81, 95124Catania, Italy
G. Rappazzo
Affiliation:
Dipartimento di Scienze Biologiche, Geologiche e Ambientali, Sez. Biologia Animale ‘M. La Greca’, Università degli Studi di Catania, Via Androne 81, 95124Catania, Italy
T. Panzavolta
Affiliation:
Dipartimento di Biotecnologie Agrarie, Università degli Studi di Firenze, Via Maragliano, 75-77, 50144Firenze, Italy
G. Curto
Affiliation:
Servizio Fitosanitario dell'Emilia Romagna, via di Corticella,133, 40128Bologna, Italy
R. Sorino
Affiliation:
Dipartimento di Biologia, Università degli Studi di Bari ‘Aldo Moro’, Via Amendola 165/A, 70126Bari, Italy
M. Oreste
Affiliation:
Dipartimento di Scienze del Suolo della Pianta e degli Alimenti (Di.S.S.P.A.), Università degli Studi di Bari ‘Aldo Moro’, Via Amendola 165/A, 70126Bari, Italy
A. Longo
Affiliation:
Dipartimento di Scienze Biologiche, Geologiche e Ambientali, Sez. Biologia Animale ‘M. La Greca’, Università degli Studi di Catania, Via Androne 81, 95124Catania, Italy
D. Leone
Affiliation:
Dipartimento di Scienze Biologiche, Geologiche e Ambientali, Sez. Biologia Animale ‘M. La Greca’, Università degli Studi di Catania, Via Androne 81, 95124Catania, Italy
R. Tiberi
Affiliation:
Dipartimento di Biotecnologie Agrarie, Università degli Studi di Firenze, Via Maragliano, 75-77, 50144Firenze, Italy
M.T. Vinciguerra
Affiliation:
Dipartimento di Scienze Biologiche, Geologiche e Ambientali, Sez. Biologia Animale ‘M. La Greca’, Università degli Studi di Catania, Via Androne 81, 95124Catania, Italy
O. Triggiani
Affiliation:
Dipartimento di Scienze del Suolo della Pianta e degli Alimenti (Di.S.S.P.A.), Università degli Studi di Bari ‘Aldo Moro’, Via Amendola 165/A, 70126Bari, Italy
*
*Fax: +39957306046 E-mail: [email protected]
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Abstract

An investigation was carried out on the distribution and biodiversity of steinernematid and heterorhabdtid entomopathogenic nematodes (EPN) in nine regions of Italy in the period 1990–2010. More than 2000 samples were collected from 580 localities and 133 of them yielded EPN specimens. A mapping of EPN distribution in Italy showed 133 indigenous EPN strains belonging to 12 species: 43 isolates of Heterorhabditis bacteriophora, 1 of H. downesi, 1 of H. megidis, 51 of Steinernema feltiae, 12 of S. affine, 4 of S. kraussei, 8 of S. apuliae, 5 of S. ichnusae, 3 of S. carpocapsae, 1 of S. vulcanicum, 3 of Steinernema ‘isolate S.sp.MY7’ of ‘S. intermedium group’ and 1 of S.arenarium. Steinernematids are more widespread than heterorhabditids and S. feltiae and H. bacteriophora are the most commonly encountered species. Sampling sites were grouped into 11 habitats: uncultivated land, orchard, field, sea coast, pinewood, broadleaf wood, grasslands, river and lake borders, caves, salt pan and moist zones; the soil texture of each site was defined and the preferences of habitat and soil texture of each species was assessed. Except for the two dominant species, S. feltiae and H. bacteriophora, EPN occurrence tends to be correlated with a specific vegetation habitat. Steinernema kraussei, H. downesi and H. megidis were collected only in Sicily and three of the species recently described – S. apuliae, S. ichnusae and S. vulcanicum – are known only from Italy and seem to be endemic.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 3 , May 2015 , pp. 359 - 366
Copyright
Copyright © Cambridge University Press 2014 

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References

Adams, B.J. & Nguyen, K.B. (2002) Taxonomy and systematics. pp. 1–34 in Gaugler, R. (Ed.) Entomopathogenic nematology. Wallingford, Oxon, CABI Publishing.Google Scholar
Almanoufi, A., Jamal, M., De Lillo, E., Tarasco, E. & Yaseen, T. (2012) A survey of the native entomopathogenic nematodes and fungi in the soil of southern region of Syria. Jordan Journal of Agricultural Sciences 8, 358–366.Google Scholar
Barbercheck, M.E. & Kaya, H.K. (1991) Effect of host condition and soil texture on host finding by the entomogenous nematodes Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) and Steinernema carpocapsae (Rhabditida: Steinernematidae). Environmental Entomology 20, 582–589.CrossRefGoogle Scholar
Cabanillas, H.E. & Raulston, J.R. (1994) Evaluation of the spatial pattern of Steinernema riobravis in corn plots. Journal of Nematology 26, 25–31.Google ScholarPubMed
Campbell, J.F., Lewis, E., Yoder, F. & Gaugler, R. (1995) Entomopathogenic nematode (Heterorhabditidae and Steinernematidae) seasonal population dynamics and impact on insect populations in turfgrass. Biological Control 5, 598–606.CrossRefGoogle Scholar
Campbell, J.F., Orza, G., Yoder, F., Lewis, E. & Gaugler, R. (1998) Spatial and temporal distribution of endemic and released entomopathogenic nematodes in turfgrass. Entomologia Experimentalis et Applicata 86, 1–11.CrossRefGoogle Scholar
Campos-Herrera, R., Escuer, M., Cuadra, L., Labrador, S., Robertson, L., Barrios, L. & Gutiérrez, C. (2007) Distribution of entomopathogenic nematodes from La Rioja (Northern Spain). Journal of Invertebrate Pathology 95, 125–139.CrossRefGoogle ScholarPubMed
Campos-Herrera, R., Gómez-Ros, J.M., Escuer, M., Cuadra, L., Barrios, L. & Gutiérrez, C. (2008) Diversity, occurrence, and life characteristics of natural EPN population from La Rioja (Northern Spain) under different levels of disturbance associated with agricultural management and their relationships with soil factors. Soil Biology and Biochemistry 40, 1474–1484.CrossRefGoogle Scholar
Canhilal, R., Reid, W., Kutuk, H. & El-Bouhssini, M. (2006) Natural occurrence of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) in Syrian soils. Research Journal of Agriculture and Biological Sciences 2, 493–497.Google Scholar
Clausi, M. & Vinciguerra, M.T. (2005) I nematodi entomopatogeni in un progetto per lo sviluppo sostenibile dei castagneti. Nematologia Mediterranea 33, 91–94.Google Scholar
Clausi, M. & Vinciguerra, M.T. (2007) Efficacy and environmental impact of entomopathogenic nematodes used against nut insect pests in some chestnut woods on Etna (Italy). IOBC-WPRS Bulletin 30, 33–37.Google Scholar
Clausi, M., Longo, A., Rappazzo, G., Tarasco, E. & Vinciguerra, M.T. (2011) Steinernema vulcanicum n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode species from Sicily (Italy). Nematology 13, 409–423.Google Scholar
Courtney, W.D., Polley, D. & Miller, V.I. (1955) TAF, an improved fixative in nematode technique. Plant Disease Report 39, 570–571.Google Scholar
Deseö, K.V., Fantoni, P. & Lazzari, G.L. (1988) Presenza di nematodi entomopatogeni (Steinernema spp. Heterorhabditis spp.) nei terreni agricoli d'Italia. Atti giornate fitopatologiche 2, 269–280.Google Scholar
Dolinski, C., Kamitani, F.L., Machado, I.R. & Winter, C.E. (2008) Molecular and morphological characterization of heterorhabditid entomopathogenic nematodes from the tropical rainforest in Brazil. Memórias do Instituto Oswaldo Cruz 103, 150–159.CrossRefGoogle ScholarPubMed
Ehlers, R.U., Deseö, K.V. & Stackerbrandt, E. (1991) Identification of Steinernema spp. and their symbiotic bacteria Xenorhabdus spp. from Italian and German soil. Nematologica 37, 360–366.Google Scholar
Eivazian, K.N., Niknam, G., Griffin, C.T., Mohammadi, S.A. & Moghaddam, M. (2009) A survey of entomopathogenic nematodes of the families Steinernematidae and Heterorhabditidae (Nematoda: Rhabditida) in the north-west of Iran. Nematology 11, 107–116.Google Scholar
Emelianoff, V., Le Brun, N., Pagès, S., Stock, S.P., Tailliez, P., Moulia, C.M. & Sicard, M. (2008) Isolation and identification of entomopathogenic nematodes and their symbiotic bacteria from Hérault and Gard (Southern France). Journal of Invertebrate Pathology 98, 211–217.CrossRefGoogle ScholarPubMed
Garcia del Pino, F. & Palomo, A. (1996) Natural occurrence of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) in Spanish soil. Journal of Invertebrate Pathology 68, 84–90.CrossRefGoogle Scholar
Glazer, I., Liran, N. & Steinberger, Y. (1991) A survey of entomopathogenic nematodes (Rhabditida) in the Negev desert. Phytoparasitica 19, 291–300.CrossRefGoogle Scholar
Glazer, I., Kozodoi, E., Salame, L. & Nestel, D. (1996) Spatial and temporal occurrence of natural populations of Heterorhabditis sp. (Nematode: Rhabditida) in a semiarid region. Biological Control 6, 130–136.CrossRefGoogle Scholar
Griffin, C.T., Downes, M.J. & Block, W. (1990) Tests of Antarctic soils for insect parasitic nematodes. Antarctic Science 2, 221–222.CrossRefGoogle Scholar
Griffin, C.T., Moore, J.F. & Downes, M.J. (1991) Occurrence of insect-parasitic nematodes (Steinernematidae, Heterorhabditidae) in the Republic of Ireland. Nematologica 37, 92–100.Google Scholar
Hatting, J., Stock, S. & Hazir, S. (2009) Diversity and distribution of entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) in South Africa. Journal of Invertebrate Pathology 102, 120–128.CrossRefGoogle ScholarPubMed
Hazir, S., Keskin, N., Stock, P., Kaya, H.K. & Özcan, S. (2003) Diversity and distribution of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) in Turkey. Biodiversity and Conservation 12, 375–386.CrossRefGoogle Scholar
Hominick, W.M. (2002) Biogeography. pp. 115–143 in Gaugler, R. (Ed.) Entomopathogenic nematology. Wallingford, Oxon, CABI Publishing.CrossRefGoogle Scholar
Hominick, W.M., Reid, A.P., Bohan, D.A. & Briscoe, B.R. (1996) Entomopathogenic nematodes – biodiversity, geographical distribution and the convention on biological diversity. Biocontrol Science and Technology 6, 317–331.CrossRefGoogle Scholar
Kary, N.E., Niknam, G., Griffin, C.T., Abolgasem, M.S. & Moghaddam, M. (2009) A survey of entomopathogenic nematodes of the families Steinernematidae and Heterorhabditidae (Nematode: Rhabditida) in the north-west of Iran. Nematology 11, 107–116.CrossRefGoogle Scholar
Kaya, H.K. & Stock, S.P. (1997) Techniques in insect nematology. pp. 281–324 in Lacey, L.A. (Ed.) Manual of techniques in insect pathology. San Diego, California, Academic Press.CrossRefGoogle Scholar
Koppenhöfer, A.M. & Kaya, H.K. (1996) Coexistence of entomopathogenic nematode species (Steinernematidae and Heterorhabditidae) with different foraging behavior. Fundamental and Applied Nematology 19, 175–183.Google Scholar
Kung, S.P., Gaugler, R. & Kaya, H.K. (1990) Soil type and entomopathogenic nematode persistence. Journal of Invertebrate Pathology 55, 401–406.CrossRefGoogle Scholar
Kuwata, R., Shigematsu, M., Yoshida, T., Yoshida, M. & Kondo, E. (2006) Phylogenetic analyses of Japanese steinernematid nematodes and their associating Xenorhabdus bacteria. Japanese Journal of Nematology 36, 75–85.Google Scholar
Longo, A. (2008) Studio della biodiversità e caratterizzazione molecolare dei Nematodi entomopatogeni presenti nei castagneti dell'Etna. Doctoral thesis, University of Catania, Catania, Italy.Google Scholar
Lòpez-Nuñez, J.C., Cano, L., Góngora-B, C.E. & Stock, S.P. (2007) Diversity and evolutionary relationships of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from central Andean region of Colombia. Nematology 9, 333–341.CrossRefGoogle Scholar
Ma, J., Chen, S., Zou, Y., Li, X., Han, R., De Clercq, P. & Moens, M. (2010) Natural occurrence of entomopathogenic nematodes in North China. Russian Journal of Nematology 18, 117–126.Google Scholar
Maru, A.K., Siddiqui, A.U., Parihar, A. & Sharma, S.K. (2007) Occurrence of entomopathogenic nematodes in varied agro-climatic zones of Rajasthan. Indian Journal of Nematology 37, 123–127.Google Scholar
Mauléon, H., Denon, D. & Briand, S. (2006) Spatial and temporal distribution of Heterorhabditis indica in their natural habitats of Guadeloupe. Nematology 8, 603–617.Google Scholar
Mráček, Z. & Bečvàr, S. (2000) Insect aggregation and entomopathogenic nematodes occurrence. Nematology 2, 297–301.Google Scholar
Mráček, Z. & Webster, J.M. (1993) Survey of Heterorhabditidae and Steinernematidae (Rhabditida: Nematoda) in Western Canada. Journal of Nematology 25, 710–717.Google ScholarPubMed
Mráček, Z., Bečvár, S., Kindlmann, P. & Jersáková, J. (2005) Habitat preference for entomopathogenic nematodes, their insect hosts and new faunistic records for the Czech Republic. Biological Control 34, 27–37.CrossRefGoogle Scholar
Nguyen, K.B. & Hunt, D.J. (2007) Entomopathogenic nematodes: systematics, phylogeny and bacterial symbionts. Nematology monographs and perspectives. Vol.5. 816 pp. Leiden, E.J. Brill Eds.CrossRefGoogle Scholar
Nguyen, K.B. & Smart, G.C. (1996) Identification of entomopathogenic nematodes in the Steinernematidae and Heterorhabditidae (Nemata: Rhabditida). Journal of Nematology 28, 286–300.Google ScholarPubMed
Nikdel, M., Niknam, G., Griffin, C.T. & Kary, N.E. (2010) Diversity of entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae) from Arasbaran forests and rangelands in north-west Iran. Nematology 12, 767–773.CrossRefGoogle Scholar
Noujeim, E., Khater, C., Pages, S., Ogier, J.-C., Tailliez, P., Hamzé, M. & Thaler, O. (2011) The first record of entomopathogenic nematodes (Rhabiditiae: Steinernematidae and Heterorhabditidiae) in natural ecosystems in Lebanon: a biogeographic approach in the Mediterranean region. Journal of Invertebrate Pathology 107, 82–85.CrossRefGoogle Scholar
Peters, A. (1996) The natural host range of Steinernema and Heterorhabditis spp. and their impact on insect populations. Biocontrol Science and Technology 6, 389–402.CrossRefGoogle Scholar
Poinar, G.O. Jr (1975) Entomogenous nematodes: a manual and host list of insect–nematode associations. 317 pp. Leiden, E.J. Brill Eds.CrossRefGoogle Scholar
Poinar, G.O. Jr (1979) Nematodes for biological control of insects. 277 pp. Boca Raton, Florida, CRC Press.Google Scholar
Poinar, G.O. Jr (1990) Taxonomy and biology of Steinernematidae and Heterorhabditidae. pp. 23–61 in Gaugler, R. & Kaya, H.K. (Eds) Entomopathogenic nematodes in biological control. Boca Raton, Florida, CRC Press.Google Scholar
Půža, V. & Mráček, Z. (2005) Seasonal dynamics of entomopathogenic nematodes of the genera Steinernema and Heterorhabditis as a response to abiotic factors and abundance of insect hosts. Journal of Invertebrate Pathology 89, 116–122.CrossRefGoogle ScholarPubMed
Rappazzo, G., Clausi, M. & Vinciguerra, M.T. (2005) Molecular identification of entomopathogenic nematodes found in pristine chestnut growing areas on the foothills of Etna (Sicily). Nematologia Mediterranea 33, 101–104.Google Scholar
Rappazzo, G., Avanzato, C., Petronio, G., Clausi, M., Tarasco, E., Buccheri, M.A., Furneri, P.M., Scuderi, M.C. & Vinciguerra, M.T. (2011) Bacterial symbionts of entomopathogenic nematodes of the genus Steinernema from South Italy: phenotypic features and evolutionary implications. IOBC-WPRS Bulletin 66, 321–325.Google Scholar
Ricci, M., Fifi, A.P., De Bernardis, M., Colli, M., Barcarotti, R. & Ragni, A. (2004) Development of a bio-insecticide based on a cold-active entomopathogenic nematode. IOBC-WPRS Bulletin 27, 139.Google Scholar
Rolston, A.N., Griffin, C.T. & Downes, M.J. (2005) Distribution of entomopathogenic nematodes in an Irish sand dune system. Nematology 7, 259–266.Google Scholar
Salame, L., Glazer, I. & Miqaia, N. (2010) Characterization of populations of entomopathogenic nematodes isolated at diverse sites across Israel. Phytoparasitica 38, 39–52.CrossRefGoogle Scholar
Seenivasan, N., Prabhu, S., Makesh, S. & Sivakumar, M. (2012) Natural occurrence of entomopathogenic nematode species (Rhabditida: Steinernematidae and Heterorhabditidae) in cotton fields of Tamil Nadu, India. Journal of Natural History 46, 2829–2843.CrossRefGoogle Scholar
Seinhorst, J.W. (1959) A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4, 67–69.CrossRefGoogle Scholar
Simard, L., Belair, G., Stock, S.P., Mauléon, H. & Dionne, J. (2007) Natural occurrence of entomopathogenic nematodes (Rhabditida: Steinernematidae) on golf courses in eastern Canada. Nematology 9, 325–332.CrossRefGoogle Scholar
Stock, S.P. & Gress, J.C. (2006) Diversity and phylogenetic relationships of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from the Sky Islands of Southern Arizona. Journal of Invertebrate Pathology 92, 66–72.CrossRefGoogle ScholarPubMed
Stock, S.P., Pryor, B.M. & Kaya, H.K. (1999) Distribution of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) in natural habitats in California. Biodiversity and Conservation 8, 535–549.CrossRefGoogle Scholar
Stock, S.P., Al Banna, L., Darwish, R. & Katbeh, A. (2008) Diversity and distribution of entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae) and their bacterial symbionts (γ-Proteobacteria: Enterobacteriaceae) in Jordan. Journal of Invertebrate Pathology 98, 228–234.CrossRefGoogle Scholar
Stuart, R.J. & Gaugler, R. (1994) Patchiness in populations of entomopathogenic nematodes. Journal of Invertebrate Pathology 64, 39–45.CrossRefGoogle Scholar
Susurluk, I.A., Tarasco, E., Triggiani, O. & Ehlers, R.-U. (2007) Molecular characterisation of entomopathogenic nematodes isolated in Italy by PCR-RFLP analysis of the ITS region of the ribosomal DNA repeat unit. Nematologia Mediterranea 35, 23–28.Google Scholar
Tarasco, E. & Triggiani, O. (1997) Survey of Steinernema and Heterorhabditis (Rhabditida: Nematoda) in Southern Italian soils. Entomologica 31, 117–123.Google Scholar
Tarasco, E. & Triggiani, O. (2005) Entomopathogenic nematodes of forest habitats in Southern Italy and their pathogenicity for overwintering larvae of Thaumetopoea pityocampa (Den. et Schiff.) (Lepidoptera, Thaumetopoeidae). pp. 257–263 in Lieutier, F. & Ghaioule, D. (Eds) Entomological Research in Mediterranean Forest Ecosystems. Institut national de la recherche agronomique (INRA, Paris, France), Editions Quae.Google Scholar
Tarasco, E. & Triggiani, O. (2007) Entomopathogenic nematodes and forest insects in Italy. Working Group ‘Insect Pathogens and Insect Parasitic Nematodes’. Proceedings of the 10th European Meeting at Locorotondo, Bari (Italy), 23–29 June 2005. IOBC-WPRS Bulletin 30, 63–65.Google Scholar
Tarasco, E., Mráček, Z., Nguyen, K.B. & Triggiani, O. (2008) Steinernema ichnusae sp. n. (Nematoda: Steinernematidae) a new entomopathogenic nematode from Sardinia Island (Italy). Journal of Invertebrate Pathology 99, 173–185.CrossRefGoogle Scholar
Tarasco, E., Clausi, M., Rappazzo, G., Vinciguerra, M., Longo, A. & Triggiani, O. (2009) Could Italy be considered a favorite place in Europe for EPN biodiversity? IOBC-WPRS Bulletin 45, 387–389.Google Scholar
Torr, P., Heritage, S. & Wilson, M.J. (2007) Steinernema kraussei, an indigenous nematode found in coniferous forest: efficacy and filed persistence against Hylobius abietis . Agricultural and Forest Entomology 9, 181–188.CrossRefGoogle Scholar
Triggiani, O. & Tarasco, E. (1998) Nematodi e funghi entomopatogeni nei terreni dell'Italia meridionale. Atti XVIII Congresso Nazionale Italiano di Entomologia, Maratea (PZ), 21–26 June p. 244.Google Scholar
Triggiani, O. & Tarasco, E. (2000) Indagini sui nematodi entomopatogeni (Rhabditida: Steinernematidae e Heterorhabditidae) in pinete e querceti dell'Italia meridionale. Entomologica 34, 23–32.Google Scholar
Triggiani, O. & Tarasco, E. (2002) Efficacy and persistence of entomopathogenic nematodes in controlling larval populations of Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae). Biocontrol Science and Technology 12, 747–752.CrossRefGoogle Scholar
Triggiani, O., Mráček, Z. & Reid, A. (2004) Steinernema apuliae sp. n. (Rhabditida: Steinernematidae): a new entomopathogenic nematode from southern Italy. Zootaxa 460, 1–12.CrossRefGoogle Scholar
Vinciguerra, M.T. & Clausi, M. (2006) Biological control of chestnut insect pests by means of entomopathogenic nematodes. Advances in Horticultural Science 20, 40–44.Google Scholar
Zamoum, M., Berchiche, S., Sai, K., Triggiani, O. & Tarasco, E. (2011) Preliminary survey on the occurrence of entomopathogenic nematodes and fungi in the forest soils of Algeria. Silva Lusitana 19, 143–147.Google Scholar
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