Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-23T22:58:50.987Z Has data issue: false hasContentIssue false

Behaviour and enzyme release by Anisakis sp. larvae (Nematoda: Ascaridida)

Published online by Cambridge University Press:  18 November 2009

Bernard E. Matthews
Affiliation:
Department of Applied Zoology, University College of North Wales, Bangor, North Wales, UK

Abstract

Anisakis sp. larvae when incubated in vitro produced secretions/excretions which released the dye from an azocoll substrate, indicating protease activity. The optimal conditions for enzyme release and activity were a temperature of 37°C and a pH of 7·5. In addition the larvae showed peak behavioural activity at 37°C, the body temperature of the final, marine mammalian host. The results are discussed in relation to their possible significance to the invasion of the various hosts in the life-cycle.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Asami, K. & Inoshita, Y. (1967) Experimental anisakiasis in guinea pigs: factors influencing infection of larvae in the host. Japanese Journal of Parasitology, 16, 415422.Google Scholar
Berland, B. (1961) Nematodes from some Norwegian marine fishes. Sarsia, 2, 150.CrossRefGoogle Scholar
Croll, N. A. (1972) Behavioural activities of nematodes. Helminthological Abstracts Series B, 41, 87106.Google Scholar
Croll, N. A., Ma, K., Smith, J. M., Sukhdeo, M. V. K. & Wild, G. (1980) Phocanema decipiens: Intestinal penetration in the laboratory rat. Experimental Parasitology, 50, 145154.CrossRefGoogle ScholarPubMed
Gibson, D. I. (1970) Aspects of the development of “Herringworm” (Anisakis sp. larva) in experimentally infected rats. Nytt Magazin for Zoologi, 18, 175187.Google Scholar
Matthews, B. E. (1977) The passage of larval helminths through tissue barriers. In: Parasite Invasion (Taylor, A. E. R. & Muller, R., editors), 15th Symposium of the British Society for Parasitology, Blackwell Scientific Publications: Oxford and Edinburgh, pp. 93119.Google Scholar
Myers, B. J. (1963) The migration of Anisakis type larvae in experimental animals. Canadian Journal of Zoology, 41, 147148.CrossRefGoogle Scholar
Pearse, A. G. E. (1968) Histochemistry. Theoretical and Applied. 3rd edit. Vol. I. Churchill Limited; London.Google Scholar
Pippy, J. H. C. & Van Banning, P. (1975) Identification of Anisakis larva (1) as Anisakis simplex (Rudolphi, 1809 det. Krabbe 1878) (Nematoda: Ascaridata). Journal of the Fisheries Research Board of Canada, 32, 2932.CrossRefGoogle Scholar
Ronald, K. (1960) The effects of physical stimuli on the larval stage of Terranova decipiens (Krabbe, 1878) (Nematoda: Anisakidae). I. Temperature. Canadian Journal of Zoology, 38, 623642.CrossRefGoogle Scholar
Smith, J. W. (1974) Experimental transfer of Anisakis sp. larvae (Nematoda: Ascaridida) from one fish host to another. Journal of Helminthology, 48, 229234.CrossRefGoogle Scholar
Smith, J. W. & Wootten, R. (1975) Experimental studies on the migration of Anisakis sp. larvae (Nematoda: Ascaridida) into the flesh of herring Clupea harengus L. International Journal for Parasitology, 5, 133136.CrossRefGoogle ScholarPubMed
Smith, J. W. & Wootten, R. (1978) Anisakis and anisakiasis. Advances in Parasitology, 16, 93163.CrossRefGoogle ScholarPubMed
Van Banning, P. (1971) Some notes on a successful rearing of the herring worm, Anisakis marina 1. (Nematoda: Heterocheilidae). Journal du Conseil International pour l'Exploration de la Mer, 34, 8488.CrossRefGoogle Scholar
Van Thiel, P. H. (1976) The present state of anisakiasis and its causative worms. Tropical and Geographical Medicine, 28, 7585.Google ScholarPubMed
Vik, R. (1964) Penetration of the stomach wall by Anisakis type larvae in Porpoises. Canadian Journal of Zoology, 42, 513.CrossRefGoogle Scholar
Wootten, R. & Smith, J. W. (1975) Observational and experimental studies on the acquisition of Anisakis sp. larvae (Nematoda: Ascaridida) by trout in fresh water. International Journal for Parasitology, 5, 373378.CrossRefGoogle ScholarPubMed
Young, P. C. & Lowe, D. (1969) Larval nematodes from fish of the subfamily Anisakinae and gastrointestinal lesions in mammals. Journal of Comparative Pathology, 79, 301313.CrossRefGoogle ScholarPubMed