Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-27T22:11:12.889Z Has data issue: false hasContentIssue false
  • English
  • Français

Antigen production by encysted muscle larvae of Trichinella spiralis

Published online by Cambridge University Press:  18 November 2009

D. I. Pritchard
D. I. Pritchard
Affiliation:
Experimental Parasitology Unit, Department of Zoology, University of Nottingham, University Park, Nottingham NG7 2RD, UK
Get access Rights & Permissions [Opens in a new window]

Abstract

The production of excretory-secretory antigens by encysted muscle larvae of Trichinella spiralis has been investigated immuno-histochemically using an antiserum raised by infection in rabbits and purified both before and after conjugation by ion-exchange chromatography. The specificity of the antibody for excretory-secretory products was demonstrated by the pattern of staining of live worms in vitro and the failure of the labelled antibody to stain dead, non-metabolizing worms. Using this labelled antibody, and unlabelled antibody in the immunoperoxidase system, the presence of parasite antigen-bearing cells in close proximity to encysted muscle larvae has been demonstrated. This is believed to be the first demonstration of antigen production by encysted muscle larvae in vivo. The implications of this observation to current concepts of immunity to Trichinella spiralis are discussed.

Type
Research Papers
Information
Journal of Helminthology , Volume 59 , Issue 1 , March 1985 , pp. 71 - 77
Copyright
Copyright © Cambridge University Press 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Brandtzaeg, P. (1973 a) Conjugation of immunoglobin G with different fluorochromes. I. Characterisation by anion exchange chromatography. Scandinavian Journal of Immunology, 2, 273–290.CrossRefGoogle Scholar
Brandtzaeg, P. (1973 b) Conjugation of immunoglobulin G with different fluorochromes. II. Specific and non-specific binding properties. Scandinavian Journal of Immunology, 2, 333–348.CrossRefGoogle ScholarPubMed
Bruce, R. G. (1970) The structure and composition of the capsule of T. spiralis in host muscle. Parasitology, 60, 223–227.CrossRefGoogle Scholar
Burns, J. (1978) Recent Advances in Pathology, No. 10, Chapter 16, Churchill Livingstone: London.Google Scholar
Catty, D. (1969) The immunology of ncmatodc infections. Trichinosis in guinea pigs as a model. In: Monographs in Allergy Vol. 5, S. Kargcr: Basel, Switzerland.Google Scholar
Despommier, D. D. (1971) Immunogcnicity of newborn larvae of Trichinella spiralis. Journal of Parasitology, 57, 531–534.CrossRefGoogle Scholar
Despommier, D. D. (1975) Adaptive changes in muscle fibres infected with Trichinella spiralis. American Journal of Pathology, 78, 477–496.Google ScholarPubMed
Despommier, D. D. (1976) Musculature. In Ecological Aspects of Parasitology (editor Kennedy, C. R.) pp. 269–285. North-Holland: Amsterdam.Google Scholar
Despommier, D. D., Campbell, W. C. & Blair, L. S. (1977) The in vivo and in vitro analysis of immunity to Trichinella spiralis in mice and rats. Parasitology, 74, 109–119.CrossRefGoogle ScholarPubMed
Grencis, R. K. (1982) Immunity to Trichinella spiralis. Ph.D. thesis, University of Glasgow.Google Scholar
Hankes, L. V. & Stoner, R. D. (1958) Incorporation of DL-tyrosinc-2-14C and DL-tryptophan-2-14C by encysted Trichinella spiralis larvae. Experimental Parasitology, 7, 92–98.CrossRefGoogle ScholarPubMed
Jackson, G. J. (1959) Fluorescent antibody studies of T. spiralis infections. Journal of Infectious Diseases, 105,97–117.Google Scholar
Kennedy, M. W., Wakelin, D. & Wilson, M. M. (1979) Transplantation of adult Trichinella spiralis between hosts: worm survival and immunological characteristics of the host-parasite relationship. Parasitology, 78, 121–130.CrossRefGoogle ScholarPubMed
Perrudet-BADOUX, A. & Binaghi, R. A. (1974) Isolation and properties of a soluble antigen of T. spiralis. Immunology, 26, 1217–1223.Google Scholar
Pritchard, D. I. (1977) Immunology of trichinosis in the guinea pig. The role of immediate hypcrsensitivity. Ph. D. thesis, University of Birmingham.Google Scholar
Purkerson, J. & Despommier, D. D. (1974) In: Trichincllosis (editor Kim, C. W.) pp. 7–23. Intext Ed. Pub.: New York.Google Scholar
Ritterson, A. L. (1966) Nature of the cyst of T. spiralis. Journal of Parasitology, 52, 157–161.CrossRefGoogle Scholar
Ruitenberg, E. J. (1974) Immunological aspects of Trichinella spiralis infection in the rat. In: Trichinellosis (editor Kim, C. W.) pp. 205–212. Intext Ed. Pub.: New York.Google Scholar
Stewart, G. L. & Read, C. P. (1972) Ribonucleic acid metabolism in mouse trichinosis. Journal of Parasitology, 58, 252–256.CrossRefGoogle ScholarPubMed
Stewart, G. L. & Giannini, S. H. (1982) Sarcocystis, Trypanosoma, Toxoplasma, Brugia, Ancylostoma, and Trichinella spp.: A review of the intraccllular parasites of striated muscle. Experimental Parasitology, 53, 406–447.CrossRefGoogle ScholarPubMed
Tanner, C. E. (1970) Purification of the antigens of Trichinella spiralis larvae by continuous flow paper clcctrophorcsis and column chromatography. Experimental Parasitology, 27, 116–135.CrossRefGoogle ScholarPubMed
Teppema, J. S., Robinson, J. E. & Ruitenberg, E. J. (1973) Ultrastructural aspects of capsule formation in Trichinella spiralis infections in rats. Parasitology, 66, 291–296.CrossRefGoogle Scholar
Theiler, H., Augustine, D. W. & Spink, W. W. (1935) On the persistence of cosinophilia, and on immune reactions in human trichinosis, several years after recovery. Parasitology, 27, 345–354.CrossRefGoogle Scholar
Zeromski, J. & Jazbor, Z. (1969) Localisation of antigens of developing Trichinella spiralis larvae in the tissues of infected rats. I. Comparative studies on direct and indirect immunofluorescence. Acta Parasitologica Polonica, 17, 119–125.Google Scholar