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Administration of the nematophagous fungus Duddingtonia flagrans to goats: an evaluation of the impact of this fungus on the degradation of faeces and on free-living soil nematodes

Published online by Cambridge University Press:  21 March 2011

C. Paraud ,
R. Lorrain ,
I. Pors  and
C. Chartier
C. Paraud*
Affiliation:
Agence Nationale de Sécurité Sanitaire, Laboratoire de Niort, 60 rue de Pied de Fond, BP 3081 79012Niort Cedex, France
R. Lorrain
Affiliation:
Centre d'Expérimentation pour la Pépinière Méridionale, Domaine de la Durette, RN 7, 84140Montfavet, France
I. Pors
Affiliation:
Agence Nationale de Sécurité Sanitaire, Laboratoire de Niort, 60 rue de Pied de Fond, BP 3081 79012Niort Cedex, France
C. Chartier
Affiliation:
Agence Nationale de Sécurité Sanitaire, Laboratoire de Niort, 60 rue de Pied de Fond, BP 3081 79012Niort Cedex, France
*
*Fax: +33-5-49794219 E-mail: carine.paraud@anses.fr
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Abstract

The environmental impact of Duddingtonia flagrans, a potential biological control agent for nematode parasites, was tested in a 2-year-plot study using goat faeces. The trial assessed the impact of fungal presence on the disintegration of faeces and on non-target, free-living soil nematode populations. Three groups of goats experimentally infected by Trichostrongylus colubriformis received three different doses of D. flagrans chlamydospores (0 chlamydospores/kg body weight (BW), 0.5 × 106 chlamydospores/kg BW or 5 × 106 chlamydospores/kg BW). One hundred grams of faeces containing T. colubriformis eggs and D. flagrans chlamydospores at three different concentrations were deposited on pasture plots on four different occasions: May 2003, September 2003, June 2004 and September 2004. Faeces were weighed 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 weeks after deposit and immediately afterwards replaced to their initial positions. In addition, soil samples were taken just below faecal deposits to evaluate the impact of fungal presence on non-target free-living nematodes. Results showed that there was no treatment effect on the pellet degradation rate. Analysis of soil nematode fauna failed to demonstrate any effect of the dose rate of 0.5 × 106 chlamydospores/kg BW, while a reduction of the number of free-living nematodes was seen for the maximal chlamydospore concentration at autumn sets.

Type
Research Papers
Information
Journal of Helminthology , Volume 86 , Issue 1 , March 2012 , pp. 95 - 103
Copyright
Copyright © Cambridge University Press 2011

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