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Worm morphology of Schistosoma japonicum using confocal laser scanning microscopy

Published online by Cambridge University Press:  02 August 2011

Wei-na Zhang ,
Peng Zhang ,
Miao Liu ,
Cui-ping Ren ,
Xue-mei Jia ,
Da-ke Huang ,
Li Gui  and
Ji-jia Shen
Wei-na Zhang
Affiliation:
Department of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology
Peng Zhang
Affiliation:
Department of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology
Miao Liu
Affiliation:
Department of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology
Cui-ping Ren
Affiliation:
Department of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology
Xue-mei Jia
Affiliation:
Comprehensive Laboratory, School of Basic Medical Science, Anhui Medical University, 81# Meishan Road, Hefei, Anhui230032, People's Republic of China
Da-ke Huang
Affiliation:
Comprehensive Laboratory, School of Basic Medical Science, Anhui Medical University, 81# Meishan Road, Hefei, Anhui230032, People's Republic of China
Li Gui
Affiliation:
Comprehensive Laboratory, School of Basic Medical Science, Anhui Medical University, 81# Meishan Road, Hefei, Anhui230032, People's Republic of China
Ji-jia Shen*
Affiliation:
Department of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology
*
*E-mail: [email protected]
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Abstract

Male and female Schistosoma japonicum worms have dissimilar appearances in their final host. In this study, a morphometric and morphological assessment of whole worms derived from unisexual and mixed infections in mice was conducted using confocal laser scanning microscopy. Worms from mixed infections showed significant morphological changes between 15 and 25 days post-infection (PI). On the fifteenth day PI, 33% of males had formed the conspicuous gynecophoric canal, but only 8% of them had testicular lobes containing a few germinative cells; 13% of females had incipient ovaries with a few immature ovarian cells inside. On the twentieth day PI, the testicular lobes contained more germinative cells in all male worms, while female worms presented vitelline glands. On the twenty-fifth day PI, more germinative cells were observed in the male testicular lobes, and differentiated cells were present in the female ovaries. All worms had fully developed reproductive organs from 30 days PI onwards. Morphometric analysis showed significant differences between mixed and unisexual infections at 35 days PI. Ovaries of worms from unisexual infections contained cells in one stage of maturation and vitelline glands had undifferentiated cells. Our study of S. japonicum provides a detailed comparison of different morphological traits from worms of mixed and unisexual infections throughout development.

Type
Research Papers
Information
Journal of Helminthology , Volume 86 , Issue 3 , September 2012 , pp. 317 - 322
Copyright
Copyright © Cambridge University Press 2011

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References

Basch, P.F. (1990) Why do schistosomes have separate sexes? Parasitology Today 6, 160–163.Google Scholar
Basch, P.F. & Nicolas, C. (1989) Schistosoma mansoni: pairing of male worms with artificial surrogate females. Experimental Parasitology 68, 202–207.CrossRefGoogle ScholarPubMed
Beckmann, S., Quack, T., Burmeister, C., Buro, C., Long, T., Dissous, C. & Grevelding, C.G. (2010a) Schistosoma mansoni: signal transduction processes during the development of the reproductive organs. Parasitology 137, 497–520.Google Scholar
Beckmann, S., Buro, C., Dissous, C., Hirzmann, J. & Grevelding, C.G. (2010b) The Syk kinase SmTK4 of Schistosoma mansoni is involved in the regulation of spermatogenesis and oogenesis. PLoS Pathogens 6, e1000769.Google Scholar
Biolchini, C.L., Neves, R.H., Hulstijn, M., Gomes, D.C. & Machado-Silva, J.R. (2006) Development of Schistosoma mansoni worms in mice analyzed by bright field and confocal microscopy. Memorias do Instituto Oswaldo Cruz 101, 261–265.Google Scholar
Fitzpatrick, J.M. & Hoffmann, K.F. (2006) Dioecious Schistosoma mansoni express divergent gene repertories regulated by pairing. International Journal for Parasitology 36, 1081–1089.CrossRefGoogle Scholar
Fitzpatrick, J.M., Peak, E., Perally, S., Chalmers, I.W., Barrett, J., Yoshino, T.P., Ivens, A.C. & Hoffmann, K.F. (2009) Anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses. PLoS Neglected Tropical Diseases 3, e543.CrossRefGoogle ScholarPubMed
Gray, D.J., Williams, G., Li, Y. & McManus, D.P. (2008) Transmission dynamics of Schistosoma japonicum in the lakes and marshlands of China. PLoS One 3, e4058.Google Scholar
Kines, K.J., Rinaldi, G., Okatcha, T.I., Morales, M.E., Mann, V.H., Tort, J.F. & Brindley, P.J. (2010) Electroporation facilitates introduction of reporter transgenes and virions into schistosome eggs. PLoS Neglected Tropical Diseases 4, e593.CrossRefGoogle ScholarPubMed
Kunz, W. (2001) Schistosome male–female interaction: induction of germ-cell differentiation. Trends in Parasitology 17, 227–231.Google Scholar
Lamb, E.W., Walls, C.D., Pesce, J.T., Riner, D.K., Maynard, S.K., Crow, E.T., Wynn, T.A., Schaefer, B.C. & Davies, S.J. (2010) Blood fluke exploitation of non-cognate CD4+T cell helps to facilitate parasite development. PLoS Pathogens 6, e1000892.CrossRefGoogle Scholar
LoVerde, P.T., Andrade, L.F. & Oliveira, G. (2009) Signal transduction regulates schistosoma reproductive biology. Current Opinion in Microbiology 12, 422–428.CrossRefGoogle ScholarPubMed
McConnell, G. (2006) Optimizing laser source operation for confocal and multiphoton laser scanning microscopy. Current Protocols in Cytometry 2, 2–13.Google Scholar
Neves, R.H., Pereira, M.J., de Oliveira, R.M., Gomes, D.C. & Machado-Silva, J.R. (1998) Schistosoma mansoni Sambon, 1907: morphometric differences between adult worms from sympatric rodent and human isolates. Memorias do Instituto Oswaldo Cruz 93 (Suppl.), 309–312.Google Scholar
Neves, R.H., Machado-Silva, J.R., Pelajo-Machado, M., Oliveira, S.A., Coutinho, E.M., Lenzi, H.L. & Gomes, D.C. (2001) Morphological aspects of Schistosoma mansoni adult worms isolated from nourished and undernourished mice: a comparative analysis by confocal laser scanning microscopy. Memorias do Instituto Oswaldo Cruz 97, 1013–1016.CrossRefGoogle Scholar
Neves, R.H., Costa-Silva, M., Martinez, E.M., Branquinho, T.B., de Oliveira, R.M., Lenzi, H.L., Gomes, D.C. & Machado-Silva, J.R. (2004) Phenotypic plasticity in adult worms of Schistosoma mansoni (Trematoda: Schistosomatidae) evidenced by brightfield and confocal laser scanning microscopies. Memorias do Instituto Oswaldo Cruz 99, 131–136.CrossRefGoogle ScholarPubMed
Neves, R.H., de Lamare Biolchini, C., Machado-Silva, J.R., Carvalho, J.J., Branquinho, T.B., Lenzi, H.L., Hulstijn, M. & Gomes, D.C. (2005) A new description of the reproductive system of Schistosoma mansoni (Trematoda: Schistosomatidae) analyzed by confocal laser scanning microscopy. Parasitology Research 95, 43–49.CrossRefGoogle ScholarPubMed
Paddock, S.W. (1996) Further developments of the laser scanning confocal microscope in biomedical research. Proceedings Society Experimental Biology Medicine 213, 24–31.CrossRefGoogle ScholarPubMed
Ribeiro-Paes, J.T. & Rodrigues, V. (1997) Sex determination and female reproductive development in the genus Schistosoma: a review. Revista Instituto Medicina Tropical São Paulo 39, 337–344.Google Scholar
Shaw, J.R. & Erasmus, D.A. (1981) Schistosoma mansoni: an examination of the reproductive status of females from single sex infections. Parasitology 82, 121–124.Google Scholar
Steinmann, P., Keiser, J., Bos, R., Tanner, M. & Utzinger, J. (2006) Schistosomiasis and water resources development: systematic review, meta-analysis, and estimates of people at risk. Lancet Infectious Diseases 6, 411–425.CrossRefGoogle ScholarPubMed
Zhang, W.N., Huang, D.K., Zhang, P., Ren, C.P., Wang, X.N., Zhang, Y.X. & Shen, J.J. (2008) Morphological observation on the reproductive system of adult Schistosoma japonicum under confocal laser scanning microscopy. Chinese Journal of Parasitology and Parasitic Diseases 30, 392–394(in Chinese).Google Scholar
Zhao, Z.R., Lei, L., Liu, M., Zhu, S.C., Ren, C.P., Wang, X.N. & Shen, J.J. (2008) Schistosoma japonicum: inhibition of Mago nashi gene expression by shRNA-mediated RNA interference. Experimental Parasitology 119, 379–384.Google Scholar