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The use of large and small subunits of ribosomal DNA in evaluating phylogenetic relationships between species of Cornudiscoides Kulkarni, 1969 (Monogenoidea: Dactylogyridae) from India

Published online by Cambridge University Press:  28 March 2016

J. Verma
Affiliation:
Department of Zoology, University of Lucknow, Lucknow- 226 007, UP, India
N. Agrawal*
Affiliation:
Department of Zoology, University of Lucknow, Lucknow- 226 007, UP, India
A.K. Verma
Affiliation:
Department of Zoology, University of Lucknow, Lucknow- 226 007, UP, India
*

Abstract

Two partial regions of ribosomal DNA (28S and 18S) were used to evaluate genetic variations among the species of Cornudiscoides, viz. C. proximus, C. geminus and C. agarwali, all parasites of Mystus vittatus (Bagridae) from River Gomati, Ganges River basin, India. Our findings demonstrated that both the large and small ribosomal subunits are useful for species identification and genetic characterization of parasites, leading to resolution of inter/intra-relationships at generic and specific levels. The secondary structures of all three species for 28S and 18S rRNA genes contained exact pattern matches (EMPs) displaying the high degree of similarity among them. The phylogenetic analyses within the members of Dactylogyridae demonstrated that species of Cornudiscoides cluster together for 28S rRNA and 18S rRNA genes.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2016 

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