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Trichinellosis survey in the wild boar from the Toledo mountains in south-western Spain (2007–2008): molecular characterization of Trichinella isolates by ISSR-PCR

Published online by Cambridge University Press:  01 June 2009

R.N. García-Sánchez ,
J.J. Nogal-Ruiz ,
R. Manzano-Lorenzo ,
J.M. Arroyo Díaz ,
G. Pérez López ,
F.J. Sánchez Ruano ,
A. Ramiro Casas ,
C. Crespo Bascón ,
F. Bolás-Fernández  and
A.R. Martínez-Fernández
R.N. García-Sánchez
Affiliation:
Depto. de Parasitología, Facultad de Farmacia, UCM, 28040Madrid, Spain
J.J. Nogal-Ruiz
Affiliation:
Depto. de Parasitología, Facultad de Farmacia, UCM, 28040Madrid, Spain
R. Manzano-Lorenzo
Affiliation:
Depto. de Parasitología, Facultad de Farmacia, UCM, 28040Madrid, Spain
J.M. Arroyo Díaz
Affiliation:
Delegación Provincial de Salud y Medio Ambiente, c/ Subida de la Granja N° 10, 45071, Toledo, Spain
G. Pérez López
Affiliation:
Delegación Provincial de Salud y Medio Ambiente, c/ Subida de la Granja N° 10, 45071, Toledo, Spain
F.J. Sánchez Ruano
Affiliation:
Delegación Provincial de Salud y Medio Ambiente, c/ Subida de la Granja N° 10, 45071, Toledo, Spain
A. Ramiro Casas
Affiliation:
Delegación Provincial de Salud y Medio Ambiente, c/ Subida de la Granja N° 10, 45071, Toledo, Spain
C. Crespo Bascón
Affiliation:
Delegación Provincial de Salud y Medio Ambiente, c/ Subida de la Granja N° 10, 45071, Toledo, Spain
F. Bolás-Fernández*
Affiliation:
Depto. de Parasitología, Facultad de Farmacia, UCM, 28040Madrid, Spain
A.R. Martínez-Fernández
Affiliation:
Depto. de Parasitología, Facultad de Farmacia, UCM, 28040Madrid, Spain
*
*Fax: +34 91 394 18 15 E-mail: [email protected]
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Abstract

In Spain, trichinellosis represents a public health problem, with an average of five outbreaks per year, wild boar meat being the main source of infection. A trichinellosis survey (2007–2008 hunting campaign) was carried out on wild boars in the Toledo Mountains (south-western Spain, EU) in the context of a surveillance programme on wildlife diseases. A total of 2216 wild boars from different locations of the region were examined. The examination was carried out by veterinarians in the local abattoir (Matadero Municipal de Toledo). The positive samples were sent to the Department of Parasitology (Facultad de Farmacia, UCM) for experimental isolation and specific identification by inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR). Using this technique we identified 17 isolates as Trichinella spiralis with an electrophoretic profile indistinguishable from the T. spiralis reference strain (ISS48). We confirmed that ISSR-PCR is a robust technique for the molecular identification of Trichinella isolates. According to our results, the prevalence of T. spiralis in wild boars from the Toledo Mountains (>800 m above sea level) during the hunting season was approximately 0.77%. The prevalence of T. spiralis (100% of our observations) is a good example of the persistence of this species in sylvatic conditions (coming from the domestic cycle), if a good wild host is abundant. Our observations confirm the major prevalence of T. spiralis over T. britovi in this region, as well as the risk to human health represented by the consumption of uninspected wild boar meat.

Type
Research Papers
Information
Journal of Helminthology , Volume 83 , Special Issue 2: Symposium and related papers from the 10th European Multicolloquium of Parasitology , June 2009 , pp. 117 - 120
Copyright
Copyright © Cambridge University Press 2009

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