Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-12-01T02:09:32.613Z Has data issue: false hasContentIssue false
  • English
  • Français

Trichinella spiralis: specificity of ES antigens from pre-encysted larvae

Published online by Cambridge University Press:  05 June 2009

R. C. Ko  and
T. P. Wong
R. C. Ko
Affiliation:
Department of Zoology, University of Hong Kong, Hong Kong
T. P. Wong
Affiliation:
Department of Zoology, University of Hong Kong, Hong Kong
Get access Rights & Permissions [Opens in a new window]

Abstract

Excretory/secretory (ES) antigens were obtained by culturing pre-encysted Trichinella spiralis larvae which were recovered from muscles of experimentally infected mice 14–15 days postinfection. Analyses of these antigens (PEL ES) with immunoblotting, SDS-PAGE and Triple Antibody ELISA showed that they yielded a low sensitivity and specificity when tested antisera against the common nematodes of Chinese pigs. As compared to ES antigens from encysted larvae. PEL ES also contained more low molecular mass proteins.

Keywords

Trichinella spiralispre-encysted larvaeES antigensspicificityNematodaimmunology
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 1 , March 1992 , pp. 38 - 44
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Despommier, D. D., Gold, A. M., Buck, S. W., Capo, V. & Silberstein, D. (1990) Trichinella spiralis: secreted antigen of the infective L1 larva localizes to the cytoplasm and nucleoplasm of infected host cells. Experimental Parasitology, 71, 27–38.CrossRefGoogle Scholar
Gamble, H. R. & Graham, C. E. (1984) Monoclonal antibody-purified antigen for the immunodiagnosis of trichinosis. American Journal of Veterinary Research, 45, 67–74.Google ScholarPubMed
Gamble, H. R., Anderson, W. R., Graham, C. E. & Murrell, K. D. (1983) Diagnosis of swine trichinosis by enzyme-linked immunosorbent assay (ELISA) using an excretory-secretory antigen. Veterinary Parasitology, 13, 349–361.CrossRefGoogle ScholarPubMed
Gamble, H. R., Rapic, D., Mariunculic, A. & Murrell, K. D. (1988) Influence of cultivation conditions on specificity of excretory-secretory antigens for the immunodiagnosis of trichinellosis. Veterinary Parasitology, 30, 131–137.CrossRefGoogle Scholar
Ko, R. C. & Yeung, M. H. F. (1989) Specificity of ES antigens in detection of Trichinella spiralis antibodies in Chinese pigs. Tropical Biomedicine, 6, 99–111.Google Scholar
Ko, R. C. & Yeung, M. H. F. (1991) Isolation of specific antigens from Trichinella spiralis by the rotating horizontal ampholine column method. Parasitology Research, 77, 255–259.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685.CrossRefGoogle ScholarPubMed
Lee, D. L., Ko, R. C., Yi, X. Y. & Yeung, M. H. F. (1991) Trichinella spiralis: antigenic epitopes from the stichocytes detected in the hypertrophic nuclei and cytoplasm of the parasitized muscle fibre (nurse cell) of the host. Parasitology, 102, 117–123.CrossRefGoogle ScholarPubMed
Towbin, H., Staehelin, T. & Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gets to nitrocellulose sheets: procedure and applications. Proceedings of the National Academy of Sciences, USA, 76, 4350–4354.CrossRefGoogle Scholar