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A Technique for Obtaining Axenic Cultures of Rhabditid Nematodes

Published online by Cambridge University Press:  05 June 2009

W. L. Nicholas  and
M. G. McEntegart
W. L. Nicholas
Affiliation:
Department of Zoology, University of Liverpool
M. G. McEntegart
Affiliation:
Public Health Laboratory, Liverpool
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Extract

Two methods of setting up axenic cultures of several species of Rhabditinae are described. Both methods depend on the killing and superficial sterilisation of gravid females with a chemcal steriliising agent, and their transferance to an innocuous medium containing antibiotics. The young worms which hatch from the eggs contained in the dead female worms are then collected aseptically. In one method merthiolate is used as a sterilising agent and in the other hydrogen peroxide.

The effectiveness of both methods in freeing the young worms from contaminating micro-organisms has been evaluated by inoculating standard bacteriological culture media with the worms.

Starting with such young worms, bacteria-free cultures have been set up using media previously described by Dougherty and his coworkers for the cultivation of free-living nematodes.

Type
Research Article
Information
Journal of Helminthology , Volume 31 , Issue 3 , September 1957 , pp. 135 - 144
Copyright
Copyright © Cambridge University Press 1957

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References

Dougherty, E. C., 1951.—“The axenic cultivation of Rhabditis briggsae Dougherty and Nigon, 1949 (Nematoda: Rhabditidae). II. Some sources and characteristics of ‘Factor Rb’.” Exp. Parasit., 1, 34–45.CrossRefGoogle Scholar
Dougherty, E. C. 1953a.—“The axenic cultivation of Rhabditis briggsae Dougherty and Nigon, 1949 (Nematoda: Rhabditidae). III. Liver preparations with various supplementation.” J. Parasit., 39, 371–380. (W.L. 11428)CrossRefGoogle ScholarPubMed
Dougherty, E. C. 1953b.—“Axenizing and monoxenizing soil nematodes.” J. Parasit., 39, (4, Sect. 2), Suppl., p. 33.Google Scholar
Dougherty, E. C. and Calhoun, H. G., 1948a.—“Techniques for temporarily freeing soil nematodes from bacteria by the use of antibiotics and merthiolate.” Anat. Rec., 100, 395. (W.L. 763)Google ScholarPubMed
Dougherty, E. C. and Calhoun, H. G., 1948b.—“Experiences in culturing Rhabditis pellio (Schneider, 1866) Butschli, 1873 (Nematoda: Rhabditidae), and related soil nematodes.” Proc. helm. Soc. Wash., 15, 55–68. (W.L. 16747a)Google Scholar
Dougherty, E. C. and Keith, D. F., 1953.—“The axenic cultivation of Rhabditis briggsae Dougherty and Nigon, 1949 (Nematoda: Rhabditidae). IV. Plasma protein fractions with various supplementation.” J. Parasit., 39, 381–384. (W.L. 11428)CrossRefGoogle ScholarPubMed
Dougherty, E. C., Raphael, J. C. Jr., and Alton, C. H., 1950.—”The axenic cultivation of Rhabditis briggsae Dougherty and Nigon, 1949 (Nematoda: Rhabditidae). I. Experiments with chick embryo juice and chemically defined media.” Proc. helm. Soc. Wash., 17, 1–10. (W.L. 16747a)Google Scholar
Mackie, T. J. and Mccartney, J. E., 1953.—“Handbook of practical bacteriology.” 9th Ed.Edinburgh.Google Scholar