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Surface culture of Steinernema sp. on two solid media and their pathogenicity against Galleria mellonella

Published online by Cambridge University Press:  10 November 2021

C.I. Cortés-Martínez Open the ORCID record for C.I. Cortés-Martínez [Opens in a new window] ,
A.I. Rodríguez-Hernández ,
M.R. López-Cuellar  and
N. Chavarría-Hernández
C.I. Cortés-Martínez*
Affiliation:
Cuerpo Académico de Biotecnología Agroalimentaria, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Tulancingo de Bravo, Hidalgo, 43600, Mexico Tecnológico Nacional de México, Instituto Tecnológico del Valle de Etla, Santiago Suchilquitongo, Oaxaca, 68230, Mexico
A.I. Rodríguez-Hernández
Affiliation:
Cuerpo Académico de Biotecnología Agroalimentaria, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Tulancingo de Bravo, Hidalgo, 43600, Mexico
M.R. López-Cuellar
Affiliation:
Cuerpo Académico de Biotecnología Agroalimentaria, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Tulancingo de Bravo, Hidalgo, 43600, Mexico
N. Chavarría-Hernández*
Affiliation:
Cuerpo Académico de Biotecnología Agroalimentaria, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Tulancingo de Bravo, Hidalgo, 43600, Mexico
*
Author for correspondence: C.I. Cortés-Martínez, E-mail: [email protected]; N. Chavarría-Hernández, E-mail: [email protected]
Author for correspondence: C.I. Cortés-Martínez, E-mail: [email protected]; N. Chavarría-Hernández, E-mail: [email protected]
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Abstract

The use of native entomopathogenic nematodes as biocontrol agents is a strategy to decrease the environmental impact of insecticides and achieve sustainable agriculture crops. In this study, the effect of the surface culture of Steinernema sp. JAP1 over two solid media at 23–27°C on infective juvenile (IJ) production and pathogenicity against Galleria mellonella larvae were investigated. First, the bacterial lawn on the surface of the media with egg yolk (P2) or chicken liver (Cl) were incubated in darkness at 30°C for 48 and 72 h, and 100 surface-sterilized IJs were added. Four harvests were conducted within the next 35 days and the mean accumulated production was superior on Cl (210 × 103 IJs) than on P2 (135 × 103 IJs), but the productivity decreased up to 10% when the incubation time of the bacterial lawn was of 72 h. The mean pathogenicity of in vitro- and in vivo-produced IJs were of 47–64% and 31%, respectively. It is worth noting that none of the two solid media had a statistically significant difference in IJ pathogenicity. Considering that the maximum multiplication factor of IJs on solid media was 2108 and that the pathogenicity against G. mellonella was outstanding, Steinernema sp. has a good potential for in vitro mass production.

Keywords

Biocontrolentomopathogenic nematodesin vitro productionone-on-one assay
Type
Research Paper
Information
Journal of Helminthology , Volume 95 , 2021 , e63
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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