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Separation of viable microfilariae free of blood cells on Percoll gradients*

Published online by Cambridge University Press:  18 November 2009

R. Chandrashekar ,
U. R. Rao ,
G. R. Rajasekariah  and
D. Subrahmanyam
R. Chandrashekar
Affiliation:
Hindustan Ciba-Geigy Limited, Research Centre, Goregaon East, Bombay 400 063, India
U. R. Rao
Affiliation:
Hindustan Ciba-Geigy Limited, Research Centre, Goregaon East, Bombay 400 063, India
G. R. Rajasekariah
Affiliation:
Hindustan Ciba-Geigy Limited, Research Centre, Goregaon East, Bombay 400 063, India
D. Subrahmanyam
Affiliation:
Hindustan Ciba-Geigy Limited, Research Centre, Goregaon East, Bombay 400 063, India
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Abstract

A consistent and reproducible method is described for isolating pure populations of microfilariae of Litomosoides carinii, Brugia pahangi, B. malayi and Dipetalonema viteae, free of cells, from blood, by density gradient centrifugation on Percoll in 0.25M sucrose. The recovery of the microfilariae was 85 to 97%.

Type
Research Note
Information
Journal of Helminthology , Volume 58 , Issue 1 , March 1984 , pp. 69 - 70
Copyright
Copyright © Cambridge University Press 1984

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References

REFERENCES

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