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Purification and properties of haemoglobin from Gastrothylax crumenifer (Trematode:Paramphistomatidae)

Published online by Cambridge University Press:  05 June 2009

Jawed Siddiqui
Affiliation:
section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh–202 001, India
Ather H. Siddiqi
Affiliation:
section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh–202 001, India

Abstract

Gastrothylax crumenifer haemoglobin was isolated, purified, chromatographed, and its molecular weight determined on a calibrated Sephadex G–100 column as well as by sodium dodecyl sulphate by the former and 16 500 by the latter method. The oxygen affinity of the pigment (P50O2) was found to be 6·1 mm Hg at pH 7·4.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 1987

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