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Prosthenhystera gattii n. sp. (Digenea: Callodistomidae), a gallbladder parasite of Bryconamericus ikaa from the lower Iguazú River, described based on combined molecular and morphological evidence

Published online by Cambridge University Press:  08 May 2020

M.M. Montes Open the ORCID record for M.M. Montes [Opens in a new window] ,
J. Barneche ,
Y. Croci ,
S. Rodriguez Gil ,
S.S. Curran ,
W. Ferrari ,
J.R. Casciotta  and
S.R. Martorelli
M.M. Montes*
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), La Plata, Buenos Aires, Argentina
J. Barneche
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), La Plata, Buenos Aires, Argentina
Y. Croci
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), La Plata, Buenos Aires, Argentina
S. Rodriguez Gil
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), La Plata, Buenos Aires, Argentina
S.S. Curran
Affiliation:
School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, Alabama, USA
W. Ferrari
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), La Plata, Buenos Aires, Argentina
J.R. Casciotta
Affiliation:
División Zoología Vertebrados, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina
S.R. Martorelli
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), La Plata, Buenos Aires, Argentina
*
Author for correspondence: M.M. Montes, E-mail: [email protected]
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Abstract

Adult forms of members of the Callodistomidae always parasitize the gallbladder of freshwater fishes and occur in Africa and America. This study provides a description of a new South American species belonging in Prosthenhystera from the gallbladder of a characid fish (Bryconamericus ikaa), and ribosomal gene sequences (28S rDNA and ITS1-5.8S-ITS2) are used to demonstrate molecular differences between the new species and congeners as well as explore interrelationships among congeners. Additionally, the first cytological analysis is conducted for a member of the family to determine chromosome number and arrangement. Prosthenhystera gattii n. sp. most closely resembles Prosthenhystera caballeroi in morphology, but the vitellarium is more extensive reaching anterior to the caecal bifurcation in the new species and the uterus is confined to the hindbody in P. gattii n. sp., whereas it extends to the level of the pharynx in P. caballeroi. Also, the testes, cirrus sac, seminal receptacle and the ratio of body length to width are larger in P. gattii n. sp. Independent Bayesian inference analyses of 28S rDNA and ITS1-5.8S-ITS2 sequence fragments produced phylograms that showed P. gattii n. sp. is more similar to Prosthenhystera obesa + Prosthenhystera oonastica than P. caballeroi + two unidentified species of Prosthenhystera, but with poor posterior probability support for the node in the ITS1-5.8S-ITS2-based phylogram. Further, the genetic distance between P. oonastica and P. gattii n. sp. are the largest among Prosthenhystera spp. Cytological analysis revealed ten metacentric chromosomes, which is fewer than the 12–18 chromosomes present in species from the closely related Gorgoderidae.

Keywords

ProsthenhysteraCallodistomidaeIguazuArgentinaBryconamericusribosomal DNA sequencesmorphologycytogenetics
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e151
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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References

Bray, RA (2002) Family callodistomidae odhner, 1910. pp. 256–260in Gibson, DI, Jones, A and Bray, RA (Eds) Keys to the Trematoda. Vol. 1. Wallingford, UK, CABI Publishing and The Natural History Museum.Google Scholar
Britt, HG (1917) Chromosomes of digenetic Trematodes. American Naturalist 81, 276–296.CrossRefGoogle Scholar
Castresana, J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology Evolution 17, 540–552.CrossRefGoogle ScholarPubMed
Curran, SS, Tkach, VV and Overstreet, RM (2006) A review of Polylekithum Arnold 1934 and its familial affinities using morphological and molecular data, with description of Polylekithum catahoulensis sp. nov. Acta Parasitologica 51, 238–248.CrossRefGoogle Scholar
Diesing, KM (1850) Systema Helminthum. Vol. 1. 680 pp. Vindobonae (Vienna), Wilhelmum Braumuller.Google Scholar
Drummond, AJ, Ashton, B, Buxton, S, Cheung, M, Cooper, A, Duran, C and Wilson, A (2016) Geneious v5.0.4. Available at http://www.geneious.com/ (accessed 29 March 2020).Google Scholar
Jiménez-Guzmán, F (1973) Trematodos digeneos de peces dulce acuicolas de Nuevo Leon, Mexico I. Dos nuevas especies y un registro Nuevo en el caracido Astyanax fasciatus mexicanus (Filippi). Cuadernos del Instituto de Investigaciones Cientificas 17, 1–19.Google Scholar
Katoh, K and Standley, DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology Evolution 30, 286–298.CrossRefGoogle ScholarPubMed
Kohn, A, Fernandes, BMM and Baptista-Farias, M (1997) Redescription of Prosthenhystera obesa (Diesing, 1850) (Callodistomidae, Digenea) with new host records and data on morphological variability. Memorias del Instituto Oswaldo Cruz 92, 171–179.CrossRefGoogle Scholar
Kohn, A, Fernandes, BMM and Cohen, SC (2007) South American trematodes parasites of fishes. 1st edn.309 pp. Rio de Janeiro, FIOCRUZ, Oswaldo Cruz Institute.Google Scholar
Lanfear, R, Calcott, B, Ho, S and Guindon, S (2012) Partition finder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology Evolution 29, 1695–1701.CrossRefGoogle Scholar
Lanfear, R, Calcott, B, Kainer, D, Mayer, C and Stamatakis, A (2014) Selecting optimal partitioning schemes for phylogenomic datasets. BMC Evolutionary Biology 14, 1–82.CrossRefGoogle ScholarPubMed
Martínez-Aquino, A, Ceccarelli, FS and Perez-Ponce de Leon, G (2013) Molecular phylogeny of the genus Margotrema (Digenea: Allocreadiidae), parasitic flatworms of goodeid freshwater fishes across central Mexico: species boundaries, host-specificity, and geographical congruence. Zoological Journal of the Linnean Society 168, 1–16.CrossRefGoogle Scholar
Montes, MM, Barneche, J, Croci, Y, Rodriguez Gil, S, Curran, SS, Ferrari, W, Casciotta, JR and Martorelli, SR (2020a) Células en división mitótica de Prosthenhystera n. sp. (Digenea: Callodistomidae). Available at http://sedici.unlp.edu.ar/handle/10915/88645 (accessed 12 February 2020).Google Scholar
Montes, MM, Barneche, J, Croci, Y, Rodriguez Gil, S, Curran, SS, Ferrari, W, Casciotta, JR and Martorelli, SR (2020b) Mitotic stages of Prosthenhystera n. sp. (Digenea: Callodistomidae), a gallbladder parasite of Bryconamericus ikaa from the lower Iguazú River. Available at http://sedici.unlp.edu.ar/handle/10915/88650 (accessed 12 February 2020).CrossRefGoogle Scholar
Nei, M and Kumar, S (2000) Molecular evolution and phylogenetics. New York, Oxford University Press.Google Scholar
Olson, PD, Cribb, TH, Tkach, VV, Bray, RA and Littlewood, DTJ (2003) Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology 33, 733–755.CrossRefGoogle Scholar
Pérez-Ponce de Leon, G and Choudhury, A (2005) Biogeography of helminth parasites of freshwater fishes in Mexico: the search for patterns and processes. Journal of Biogeography 32, 645–659.CrossRefGoogle Scholar
Petkevičiute, R and Stanevičiute, G (2008) Comparative karyological analysis of three members of Allocreadiidae (Digenea): taxonomic and phylogenetic implications. Parasitology Research 103, 1105–1110.CrossRefGoogle ScholarPubMed
Petkevičiute, R, Stunženas, V and Stanevičiute, G (2004) Cytogenetic and sequence comparison of adult Phyllodistomum (Digenea: Gorgoderidae) from the three-spined stickleback with larvae from two bivalves. Parasitology 129, 771–778.CrossRefGoogle ScholarPubMed
Pritchard, MH and Kruse, GOW (1982) The collection and preservation of animal parasites. 141 pp. Nebraska: University of Nebraska Press.Google Scholar
Ronquist, F, Teslenkovan, M, van der Mark, P, et al. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across large model space. Systematic Biology 61, 539–542.CrossRefGoogle ScholarPubMed
Rosas-Valdez, R, Choudhury, A and Perez-Ponce de Leon, G (2011) Molecular prospecting for cryptic species in Phyllodistomum lacustri (Platyhelminthes, Gorgoderidae). Zoologica Scripta 40, 296–305.CrossRefGoogle Scholar
Schneider, CA, Rasband, WS and Eliceiri, KW (2012) NIH image to ImageJ: 25 years of image analysis. Nature Methods 9, 671–675.CrossRefGoogle ScholarPubMed
Schwarz, G (1978) Estimating the dimension of a model. Annals of Statistics 6, 461–464.CrossRefGoogle Scholar
Sofi, TA, Ahmad, F, Sheikh, BA, Mohi, O, Din, U and Fazili, KM (2015) Chromosomes and cytogenetics of helminths (Turbellaria, Trematoda, Cestoda, Nematoda and acanthocephalan). Neotropical Helminthology 9, 113–162.Google Scholar
Talavera, G and Castresana, J (2007) Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Systematic Biology 56, 564–577.CrossRefGoogle ScholarPubMed
Tamura, K, Stecher, G, Peterson, D, Filipski, A and Kumar, S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology Evolution 30, 2725–2729.CrossRefGoogle ScholarPubMed
Tkach, VS and Curran, SS (2015) Prosthenystera oonastica (Digenea: Callodistomidae) from ictalurid catfishes in southeastern United States and molecular evidence differentiating species in the genus across Americas. Systematic Parasitology 90, 39–51.CrossRefGoogle ScholarPubMed
Tkach, VV, Littlewood, DTJ, Olson, PD, Kinsella, JM and Swiderski, Z (2003) Molecular phylogenetic analysis of the Microphalloidea Ward, 1901 (Trematoda: Digenea). Systematic Parasitology 56, 1–15.CrossRefGoogle Scholar
Walton, AC (1959) Some parasites and their chromosomes. The Journal of Parasitology 45, 1–20.CrossRefGoogle ScholarPubMed