Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-27T04:03:35.082Z Has data issue: false hasContentIssue false
  • English
  • Français

Polarographic determination of mitochondrial respiration in Gastrothylax crumenifer

Published online by Cambridge University Press:  05 June 2009

Jawed Siddiqui  and
Ather H Siddiqi
Jawed Siddiqui
Affiliation:
Section of Parasitology, Deparment of Zoology, Aligarh Muslim University, Aligarh-202 001, India
Ather H Siddiqi
Affiliation:
Section of Parasitology, Deparment of Zoology, Aligarh Muslim University, Aligarh-202 001, India
Get access Rights & Permissions [Opens in a new window]

Abstract

The oxidative metabolism of Gastrothylax crumenifer mitochondrial fractions was assayed polarographically at 30°C in the presence of various substrates. Succinate was most readily oxidized, malate and pyruvate were oxidized at subsantially lower retes. The spcific inhibitors such as malonate, KCN, NaN3, oxaloacetate, salicylhydroxamic acid and oligomycin effectively affected the mitochondrial oxygen consumption. The results indicate the presence of oxidative phosphorylation and cytochrome systems. Mitochondrial ATPase was found to be active.

Keywords

Gastrothylax crumeniferrespirationoxidative phosphorylation
Type
Research Note
Information
Journal of Helminthology , Volume 61 , Issue 2 , June 1987 , pp. 143 - 145
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Benedictov, I. I. (1972) Ferricyanide and fumarate-reducing enzyme in mitochondria of helminths. Angewandte Parasitologie, 13, 28–35.Google Scholar
Bradford, M. M. (1976) A repid and sensitive method for the quanitation of microquatities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.CrossRefGoogle Scholar
Kieley, W. W. (1972) Mg-activated muscle adenosine triphosphatase. In “Methods in enzymology” (editors Collowick., S. P. and aplan, N. O..). Vol. 2. pp. 588–591. Academic Press, New York.Google Scholar
Prichard, R. K. & Schofield, P. J. (1971). Fasciola hepatica: cytochrome c oxidoreductase and effects of oxygen tension and inhibition. Experimental Parasitology, 29, 215–222.CrossRefGoogle Scholar
Shestak, E. A. (1975) Oxidative process in the tissues of Paramphistomum cervi. Vladivostok, U.S.S.R. Trydy Biologo-Pochvennogo Institut (Gel' ‘mintologicheskie lssledovaniya zhivotnkk i rastenii) Novayayaseriya, 26, 205–210.Google Scholar
Slater, E. C. (1967) Application of inhibitors and uncouplers for a study of oxidative phosphorylation. In: Methods of enzymology, (editors Estabrook., R. W. and Pullman, M. E..) vol. 10, pp. 48–57. Academic Press: New York.Google Scholar
Smyth, J. D. (1969) The physiology of cestodes. W.H. Freeman: San Francisco.Google Scholar