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Molecular identification and seasonal infections of species of Fasciola in ruminants from two provinces in China

Published online by Cambridge University Press:  30 June 2015

W. Yuan ,
J.-M. Liu ,
K. Lu ,
H. Li ,
M.-M. Duan ,
J.-T. Feng ,
Y. Hong ,
Y.-P. Liu ,
Y. Zhou  and
L.-B. Tong
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W. Yuan
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
J.-M. Liu*
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
K. Lu
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
H. Li
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
M.-M. Duan
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
J.-T. Feng
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
Y. Hong
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
Y.-P. Liu
Affiliation:
Anhui Center for Animal Disease Control and Prevention, Hefei230000, P.R. China
Y. Zhou
Affiliation:
Hubei Center for Animal Disease Control and Prevention, Wuhan430000, P.R. China
L.-B. Tong
Affiliation:
Wangjiang County Center for Animal Disease Control and Prevention, Anqing246000, P.R. China
J. Lu
Affiliation:
Gongan County Center for Animal Disease Control and Prevention, Jingzhou434000, P.R. China
C.-G. Zhu
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
Y.-M. Jin
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
G.-F. Cheng
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
J.-J. Lin
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou225009, P.R. China
*
*Fax: +86 21 34293619 E-mail: [email protected]
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Abstract

We determined the prevalence and seasonality of infections by Fasciola of goats and bovine species (cattle and water buffalo) in Hubei and Anhui provinces of China. Faecal samples were collected at 2- to 3-month intervals from 200 goats in Hubei province and from 152 bovine species in Anhui province. All faecal samples were examined for the presence of parasites. We determined the nucleotide sequences of the first and second internal transcribed spacers (ITS-1 and ITS-2) of the nuclear ribosomal DNA (rDNA) of 39 Fasciola worms from Anhui province. The prevalence of Fasciola infection in goats ranged between 3.5 and 37.0%, with mean eggs per gram (EPG) ranging between 29.0 and 166.0. Prevalence and EPG exhibited downward trends over time with significant differences. The prevalence of Fasciola infection in cattle ranged between 13.3 and 46.2% (mean EPG, 36.4–100.0), and that of water buffalo ranged between 10.3 and 35.4% (mean EPG, 25.0–89.6), with a higher prevalence of infection and EPG from June to October compared with December to March. Analysis of ITS-1 and ITS-2 sequences revealed that F. hepatica and F. gigantica were present in all bovine species of Anhui province and that F. gigantica mainly infected water buffalo. This is the first demonstration of Fasciola infection in Hubei province and detection of F. hepatica and F. gigantica in Anhui province. The present study of Hubei province shows that mass treatment of livestock with closantel sodium injections in April and August/September controlled Fasciola infection effectively.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 3 , May 2016 , pp. 359 - 363
Copyright
Copyright © Cambridge University Press 2015 

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