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Molecular characterization of the horse isolate of Echinococcus granulosus in Turkey

Published online by Cambridge University Press:  24 July 2012

A.E. Utuk  and
S. Simsek
A.E. Utuk
Affiliation:
Etlik Veterinary Control Central Research Institute, Parasitology and Bee Diseases Laboratory, 06020Ankara, Turkey
S. Simsek*
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23,119Elazig, Turkey
*
* Fax: +90,42423,88173, E-mail: [email protected]
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Abstract

Cystic echinococcosis is one of the most important helminthozoonoses, affecting various species of intermediate hosts and humans. In this report, we present Echinococcus granulosus infection in a horse and its molecular characterization. Polymerase chain reaction (PCR) amplification of mitochondrial 12S rRNA (mt-12S rRNA) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed. According to the mt-12S rRNA-PCR result, the horse isolate was grouped with E. granulosus sensu stricto (G1–G3) and the partial mt-CO1 sequence corresponded to the G1 strain. This is the first study of the molecular characterization of the horse isolate of E. granulosus in Turkey.

Type
Research Papers
Information
Journal of Helminthology , Volume 87 , Issue 3 , September 2013 , pp. 305 - 308
Copyright
Copyright © Cambridge University Press 2012 

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References

Alibasoglu, M. & Yalciner, S. (1965) 1933–1961 yılları arasında Ankara ve yöresinde atlarda görülen hastalıklara toplu bir bakış. Veterinary Journal of Ankara University 12, 98–111(in Turkish).Google Scholar
Azlaf, R. & Dakkak, A. (2006) Epidemiological study of the cystic echinococcosis in Morocco. Veterinary Parasitology 137, 83–93.CrossRefGoogle ScholarPubMed
Balkaya, I. & Simsek, S. (2011) A serological survey of cystic echinococcosis in equids in east of Turkey. Iranian Journal of Parasitology 6, 46–50.Google Scholar
Bowles, J., Blair, D. & McManus, D.P. (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology 54, 165–174.CrossRefGoogle ScholarPubMed
Cook, B.R. (1989) The epidemiology of Echinococcus granulosus in Great Britain. V. The status of subspecies of Echinococcus granulosus in Great Britain. Annals of Tropical Medicine and Parasitology 83, 51–61.CrossRefGoogle ScholarPubMed
Dinkel, A., Njoroge, E.M., Zimmermann, A., Walz, M., Zeyhle, E., Elmahdi, I.E., Mackenstedt, U. & Romig, T. (2004) A PCR system for detection of species and genotypes of the Echinococcus granulosus complex, with reference to the epidemiological situation in eastern Africa. International Journal for Parasitology 34, 645–653.CrossRefGoogle Scholar
Howkins, A.B., Gemmel, M.A. & Smyth, J.D. (1965) Experimental transmission of Echinococcus from horse to foxes. Annals of Tropical Medicine and Parasitology 59, 457–462.CrossRefGoogle ScholarPubMed
Knudsen, B., Knudsen, T., Flensborg, M., Sandmann, H., Heltzen, M., Andersen, A., Dickenson, M., Bardram, J., Steffensen, P.J., Mønsted, S., Lauritzen, T., Forsberg, R., Thanbichler, A., Bendtsen, J.D., Görlitz, L., Rasmussen, J., Tordrup, D., Værum, M., Ravn, M.N., Hachenberg, C., Fisker, E., Dekker, P., Schultz, J., Hein, A.M.K. & Sinding, J.B. (2007) CLC Main Workbench. Version 5.5. Aarhus, Denmark, CLC bio.Google Scholar
Nakao, M., Yanagida, T., Okamoto, M., Knapp, J., Nkouawa, A., Sako, Y. & Ito, A. (2010) State-of-the-art Echinococcus and Taenia: phylogenetic taxonomy of human-pathogenic tapeworms and its application to molecular diagnosis. Infection, Genetics and Evolution 10, 444–452.CrossRefGoogle ScholarPubMed
Oge, S., Kircali, F., Yildirim, A. & Oge, H. (2004) Cyst hydatid infection in equids. Veterinary Journal of Ankara University 51, 75–76(in Turkish).Google Scholar
Ponce Gordo, F. & Cuesta Bandera, C. (1998) Observations on the Echinococcus granulosus horse strain in Spain. Veterinary Parasitology 76, 65–70.CrossRefGoogle ScholarPubMed
Saitou, N. & Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406–425.Google Scholar
Simsek, S. & Eroksuz, Y. (2009) Occurrence and molecular characterization of Echinococcus granulosus in Turkish mouflon (Ovis gmelinii anatolica). Acta Tropica 109, 167–169.CrossRefGoogle ScholarPubMed
Simsek, S., Balkaya, I., Ciftci, A.T. & Utuk, A.E. (2011a) Molecular discrimination of sheep and cattle isolates of Echinococcus granulosus by SSCP and conventional PCR in Turkey. Veterinary Parasitology 178, 367–369.CrossRefGoogle ScholarPubMed
Simsek, S., Kaplan, M. & Ozercan, I.H. (2011b) A comprehensive molecular survey of Echinococcus granulosus in formalin-fixed paraffin-embedded tissues in human isolates in Turkey. Parasitology Research 109, 411–419.CrossRefGoogle ScholarPubMed
Šnábel, V., Altintas, N., D'Amelio, S., Nakao, M., Romig, T., Yolasigmaz, A., Gunes, K., Turk, M., Busi, M., Hüttner, M., Ševcová, D., Ito, A., Altintas, N. & Dubinský, P. (2009) Cystic echinococcosis in Turkey: genetic variability and first record of the pig strain (G7) in the country. Parasitology Research 105, 145–154.CrossRefGoogle ScholarPubMed
Thomson, R.C.A. (2008) The taxonomy, phylogeny and transmission of Echinococcus. Experimental Parasitology 119, 439–446.CrossRefGoogle Scholar
Thompson, R.C.A. & Lymbery, A.J. (1988) The nature, extent and significance of variation within the genus Echinococcus. Advances in Parasitology 27, 209–258.CrossRefGoogle ScholarPubMed
Thompson, R.C.A. & McManus, D.P. (2002) Towards a taxonomic revision of the genus Echinococcus. Trends in Parasitology 18, 452–457.CrossRefGoogle ScholarPubMed
Utuk, A.E. & Piskin, F.C. (2010) Cystic hydatidosis in a crossbreed mountain goat and its molecular characterization. The Journal of the Faculty of Veterinary Medicine, University of Kafkas 16, 671–673.Google Scholar
Utuk, A.E., Simsek, S., Koroglu, E. & McManus, D.P. (2008) Molecular genetic characterization of different isolates of Echinococcus granulosus in east and southeast regions of Turkey. Acta Tropica 107, 192–194.CrossRefGoogle Scholar
Utuk, A.E., Piskin, F.C. & Dalkilic, B. (2012) Molecular characterization of sheep isolates of Echinococcus granulosus in Kilis province. The Journal of the Faculty of Veterinary Medicine, University of Kafkas 18 (Suppl-A), 35–38.Google Scholar
Varcasia, A., Garippa, G., Pipia, A.P., Scala, A., Brianti, E., Giannetto, S., Battelli, G., Poglayen, G. & Micagni, G. (2008) Cystic echinococcosis in equids in Italy. Parasitology Research 102, 815–818.CrossRefGoogle ScholarPubMed
Williams, R.J. & Sweatman, G.K. (1963) On the transmission, biology and morphology of Echinococcus granulosus equinus, a new sub-species of hydatid tapeworm in horses in Great Britain. Parasitology 53, 391–407.CrossRefGoogle Scholar