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Is Saccocoelioides bacilliformis Szidat, 1973 (Digenea: Haploporidae) a valid species? Genetic and morphological studies of this controversial species

Published online by Cambridge University Press:  10 December 2024

M.M. Montes Open the ORCID record for M.M. Montes [Opens in a new window] ,
N. Arredondo Open the ORCID record for N. Arredondo [Opens in a new window] ,
Y. Croci Open the ORCID record for Y. Croci [Opens in a new window] ,
J. Barneche Open the ORCID record for J. Barneche [Opens in a new window] ,
D. Balcazar Open the ORCID record for D. Balcazar [Opens in a new window]  and
G.F. Reig Cardarella Open the ORCID record for G.F. Reig Cardarella [Opens in a new window]
M.M. Montes*
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), La Plata, Buenos Aires, Argentina
N. Arredondo
Affiliation:
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Laboratorio de Sistemática y Biología de Parásitos de Organismos Acuáticos, Buenos Aires, Argentina CONICET, Universidad de Buenos Aires, Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA), Buenos Aires, Argentina
Y. Croci
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), La Plata, Buenos Aires, Argentina
J. Barneche
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), La Plata, Buenos Aires, Argentina
D. Balcazar
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), La Plata, Buenos Aires, Argentina
G.F. Reig Cardarella
Affiliation:
Escuela de Tecnología Médica y Centro Integrativo de Biología y Química Aplicada (CIBQA). Universidad Bernardo O’ Higgins, Santiago de Chile, Chile
*
Corresponding author: M.M. Montes; Email: [email protected]
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Abstract

Saccocoelioides is a genus within the family Haploporidae and is distributed throughout the Americas. The recent application of molecular techniques has facilitated the reorganization of this genus and the description of new species, resulting in a current total of 28 species. In Argentina, 11 species have been identified; however, the validity of Saccocoelioides bacilliformis and Saccocoelioides octavus remains controversial. Recently, specimens of digeneans were collected from two distinct geographical locations: the Paraná River basin (Misiones province) and the Río de la Plata basin (Buenos Aires province). These specimens were found parasitizing two different fish species: Bryconamericus sylvicola and Bryconamericus iheringi. Sequences of the 28S and COI genes were obtained for both digeneans from the two collection sites and hosts. The morphological study, combined with genetic sequences, confirmed their assignment to the genus Saccocoelioides. The COI and 28S gene sequences obtained from specimens at both collection sites exhibited genetic distances of 0.13% and 3.41%, respectively, indicating that these Saccocoelioides specimens represent a single species. The comprehensive taxonomic study of the newly collected specimens, along with an examination of the type material of S. bacilliformis and S. octavus, allows us to confirm the validity of both species. Argentina is home to more than 550 species of freshwater fauna, and it is likely that new species of Saccocoelioides remain to be discovered in underexplored regions of the basin, such as the Uruguay, Pilcomayo and Bermejo rivers.

Keywords

digeneanMisionesLa Plata River basinCOI28Stetra fishes
Type
Research Paper
Information
Journal of Helminthology , Volume 98 , 2024 , e85
Copyright
© The Author(s), 2024. Published by Cambridge University Press

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