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Immunodiagnosis of human dirofilariasis by enzyme-linked immunosorbent assay using recombinant DNA-derived fusion protein

Published online by Cambridge University Press:  05 June 2009

S. Sun  and
K. Sugane
S. Sun
Affiliation:
Department of Parasitology, Faculty of Medicine, Shinshu University, Asahi 3-1-1, Matsumoto City, Nagano Prefecture, Japan
K. Sugane*
Affiliation:
Department of Parasitology, Faculty of Medicine, Shinshu University, Asahi 3-1-1, Matsumoto City, Nagano Prefecture, Japan
*
*Author to whom correspondence should be addressed.
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Abstract

An enzyme-linked immunosorbent assay (ELISA) using antigenic β-galactosidase-Dirofilaria immitis recombinant fusion protein (FP) obtained by the recombinant DNA technique provided a useful diagnostic tool for human dirofilariasis. D. immitis-infected human sera reacted strongly with FP that was immobilized with anti-β-galactosidase monoclonal antibody on microplates. However, the FP did not react with sera from patients with other filariasis. In detection of anti-D. immitis IgG antibody. ELISA using FP was highly sensitive and specific compared to that using crude somatic antigen.

Keywords

Dirofilaria immitisdiagnosisELISAfusion proteinNematoda
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 3 , September 1992 , pp. 220 - 226
Copyright
Copyright © Cambridge University Press 1992

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References

REFERENCES

Bemrick, W. J. & Sandholm, H. A. (1966) Aedes vexans and other potential mosquito vectors of D. immitis in Minnesota. Journal of Parasitology, 54, 762–767.CrossRefGoogle Scholar
Caravelli, J. F., Zaman, M. B. & Bains, M. S. (1981) Human pulmonary dirofilariasis mimicking metastatic disease. Clinical Bulletin, 11, 88–90.Google ScholarPubMed
Ciferri, F. (1982) Human pulmonary dirofilariasis in the United States: A critical review. American Journal of Tropical Medicine and Hygiene, 3, 302–308.Google Scholar
Dayal, Y. & Neafie, R. C. (1975) Human pulmonary dirofilariasis: A case report and review of the literature. American Review of Respiratory Disease, 112, 437–443.Google ScholarPubMed
Habets, W. J. A., Hoet, M. H., Sillekens, P. T. G., Derooij, D. J. R. A. M., Van Deputte, L. B. A. & Van Venrooij, W. J. (1989) Detection of autoantibodies in a quantitative immunoassay using recombinant ribonucleoprotein antigens. Clinical and Experimental immunology, 76, 172–177.Google Scholar
Hedge, E. C. & Ridley, D. S. (1977) Immunofluorescent reactions with microfilariae. 1. Diagnostic evaluation. Transactions of the Royal Society of Tropical Medicine and Hygiene, 71, 304–307.Google Scholar
Huynh, T. V., Young, R. A. & Davis, R. W. (1984) Constructing and screening cDNA libraries in λ gt10 and λ gt11. In: DNA Cloning (Editor Glover, D. M.) pp. 49–78. IRL Press: Oxford, UK.Google Scholar
Kohno, T., Katsumaru, H., Nakamoto, H., Yasuda, T., Mitsukawa, T., Matsukura, S., Tsunetoshi, Y. & Ishikawa, E. (1991) Use of inactive β-D-galactosidase antibodies in immune complex transfer enzyme immunoassay for antithyroglobulin IgG in serum using β-D-galactosidase from Escherichia coli as label. Journal of Clinical Laboratory Analysis, 5, 197–205.CrossRefGoogle ScholarPubMed
Merrill, J. R., Otis, J., Logan, W. D. & Davis, M. B. (1980) The dog heartworm (Dirofilaria immitis) in man: An epidemic pending or in progress? Journal of the American Medical Association, 243, 1066–1068.CrossRefGoogle ScholarPubMed
Mizrachi, H. H., Lieberman, P. H., Tolui, S. S. & Sun, T. (1989) Pulmonary dirofilariasis: Mimicry of well-differentiated squamous carcinoma. Human Pathology, 20, 818–819.CrossRefGoogle ScholarPubMed
Philipp, M. & Davis, T. B. (1986) Biochemical and immunologic characterization of a major surface antigen of Dirofilaria immitis infective larvae. Journal of Immunology, 136, 2621–2627.Google Scholar
Spieser, F. (1980) Application of the enzyme-linked immunosorbent assay (ELISA) for the diagnosis of filariasis and echinococcosis. Tropenmedizin and Parasitologie, 29, 95–102.Google Scholar
Sun, S., Matsuura, T. & Sugane, K. (1991) Molecular cloning of the cDNA encoding an immunodominant antigen of Dirofilaria immitis. Journal of Helminthology, 65, 149–158.CrossRefGoogle ScholarPubMed
Sun, S., Matsuura, T. & Sugane, K. (1992) Stage specific expression of a developmentally regulated gene in Dirofilaria immitis. Journal of Helminthology, 66, 62–67.Google Scholar
Voller, A., Bartlett, A. & Bidwell, D. E. (1976) Enzyme inmmunoassays for parasitic disease. Transactions of the Royal Society of Tropical Medicine and Hygiene, 70, 98–106.Google Scholar
Yazdanbakhsh, M. (1990) Molecular biological approaches towards immunodiagnosis of filariasis. Parasitology Today, 6, 207–208.Google Scholar
Yoshimura, H., Akao, N., Kondo, K., Ohnishi, Y., Kitagawa, M. & Kamimura, K.(1980) Human dirofilariasis in Japan; case report and review of literature. International Journal of Zoonoses, 7, 107–114.Google ScholarPubMed