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Hepatic fibrosis and Fasciola hepatica infection in cattle

Published online by Cambridge University Press:  01 December 2007

Luis A. Marcos ,
Pedro Yi ,
Alfredo Machicado ,
Roy Andrade ,
Frine Samalvides ,
Juvenal Sánchez  and
Angélica Terashima
Luis A. Marcos*
Affiliation:
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú Internal Medicine Department, University of Texas Health Science Center at Houston, Texas, USA
Pedro Yi
Affiliation:
Laboratorio de Patología, Facultad de Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Perú
Alfredo Machicado
Affiliation:
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú
Roy Andrade
Affiliation:
Laboratorio de Patología, Facultad de Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Perú
Frine Samalvides
Affiliation:
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú
Juvenal Sánchez
Affiliation:
Departamento de Patología, Instituto Nacional de Enfermedades, Neoplásicas, Lima, Perú
Angélica Terashima
Affiliation:
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú
*
*E-mail: [email protected]
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Abstract

This study focuses on the development of fibrosis of the liver of cattle with Fasciola hepatica infection, correlating with the intensity of infection. Animals with an established diagnosis of chronic F. hepatica infection were identified in a slaughterhouse in Lima, Perú. The study included 24 fresh cattle livers from infected animals and two uninfected controls. Tissues were stored at 4°C for approximately 8 h after which they were brought to a necropsy room and examined. Between 9 and 12 biopsies were randomly obtained from each liver. Histological staining of formalin-fixed liver sections with haematoxylin and eosin (H & E) and Masson's trichrome were performed. Liver samples were examined using a pathology protocol that included 30 items. Histopathologically, 16 out of 30 liver specimens (67.6%) showed diffuse fibrotic lesions (cirrhosis) with a mean number of Fasciola of 116 ± 30 (range 4–435). Pathological data were matched to number of adult parasites and presence of cirrhosis after being reviewed by two independent pathologists. There was concordance between the two pathologists (K = 0.72). The group with cirrhosis showed an average of 116 ± 30 adult parasites whereas the group not showing cirrhosis contained 56 ± 28 flukes (P = 0.2). To measure how number of flukes and diagnosis of cirrhosis are related we used Kendall's tau-b coefficient; the correlation was +0.296 (P = 0.04). Receiver Operating Characteristic (ROC) curve results showed that the best point was 38 parasite adults, which had 93.8% sensitivity and 75% specificity. We conclude that as the number of F. hepatica adult forms increases, the likelihood of developing liver fibrosis will also increase in cattle.

Type
Research Papers
Information
Journal of Helminthology , Volume 81 , Issue 4 , December 2007 , pp. 381 - 386
Copyright
Copyright © Cambridge University Press 2007

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