Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-24T13:05:07.856Z Has data issue: false hasContentIssue false
  • English
  • Français

Heligmosomoides polygyrus: effect of exogenous steroid hormones on egg output in vitro

Published online by Cambridge University Press:  05 June 2009

Tammi L. Richardson  and
Barbara M. Mackinnon
Tammi L. Richardson
Affiliation:
Department of Biology, University of New Brunswick, Fredericton, New Brunswick, Canada E3B 6E1
Barbara M. Mackinnon
Affiliation:
Department of Biology, University of New Brunswick, Fredericton, New Brunswick, Canada E3B 6E1
Get access Rights & Permissions [Opens in a new window]

Abstract

The effect of exogenous steroid hormones on the egg output of Heligmosomoides polygyrus (Nematoda: Trichostrongylidae) was examined in vitro. Using worms raised in female mice, it was found that estradiol, testosterone, and cortisone each significantly decreased egg output. Although similar trends were found using H. polygyrus raised in male mice, none of the decreases found was significant. No significant differences were found with ecdysone or progesterone treatments using worms from female or male mice. Treatment of worms with cortisone did not significantly affect retention of eggs within the uterus of H. polygyrus. Titration of the effect of cortisone on egg output indicated that levels of reduction were significant for concentrations of 5·6 × 10−6 M to 5·6 × 10−3 M in worms from female mice and for concentrations of 5·6 × 10−8, × 10−7, × 10−5 and × 10−3 in worms from male mice. Radioisotope labelling experiments showed incorporation of 3H-corticosterone in the nucleus of intestinal cells of H. polygyrus suggesting that its effect on egg production may be via a modulatory effect on the intestinal cells.

Keywords

Heligmosomoides polygyrusmouseegg outputsteroidsautoradiography
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 2 , June 1990 , pp. 123 - 132
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Altman, P. L. & Dittmer, D. S. (1961) Blood and other body fluids. p. 98. Federation of American Societies for Experimental Biology, Bethesda, Maryland.Google Scholar
Barrett, J., Cain, G. D. & Fairbairn, D. (1970) Sterols in Ascaris lumbricoides (Nematoda), Macrocanthorhynchus hirudinaceus and Moniliformis dubius (Acanthocephala), and Echinostoma revolutum (Trematoda). Journal of Parasitology, 56, 1004–1008.CrossRefGoogle ScholarPubMed
Bottjer, K. P., Weinstein, P. P. & Thompson, M. (1984a) Effects of azasteroids on growth and development of the free living stages of Nippostrongylus brasiliensis and Nematospiroides dubius. Comparative Biochemistry and Physiology, 78B, 805–811.Google ScholarPubMed
Bottjer, K. P., Whisenton, L. R. & Weinstein, P. R. (1984b) Ecdysteroid-like substances in Nippostrongylus brasiliensis. Journal of Parasitology, 72, 326–329.Google Scholar
Chitwood, D. J., Lozano, R. & Lusby, W. R. (1986) Recent developments in nematode steroid biochemistry. Journal of Nematology, 18, 9–17.Google Scholar
Chomicz, L. (1984) The effect of gonadectomy and sex hormones on the course of experimental strongyloidosis (sheep strain) in rabbits. I. Single infections. Acta Parasitologica Polonica, 29, 149–166.Google Scholar
Chung, W.-L., Parish, E. & Bone, R. W. (1986) Sex steroid content and metabolism in Trichostrongylus colubriformis. Journal of Parasitology, 72, 326–329.Google Scholar
Cole, R. J. & Dutky, S. R. (1969) A sterol requirement in Turbatrix aceti and Panagrellus redivivus. Journal of Nematology, 1, 72–75.Google Scholar
Cole, R. J. & Krusberg, L. R. (1967) Sterols in Ascaris lumbricoides. Comparative Biochemistry and Physiology, 21, 109–114.Google Scholar
Dennis, R. D. (1976) Insect morphogenetic hormones and developmental mechanisms in the nematode Nematospiroides dubius. Comparative Biochemistry and Physiology, 53A, 53–56.CrossRefGoogle ScholarPubMed
Dennis, R. D. (1977) On ecdysone binding proteins and ecdysone-like material in nematodes. International Journal for Parasitology, 7, 181–188.CrossRefGoogle ScholarPubMed
Dobson, C. (1966) Certain aspects of the host-parasite relationship of Nematospiroides dubius (Baylis). Parasitology, 56, 407–416.Google Scholar
Eckert, R. & Randall, D. (1983) Animal Physiology Mechanisms and Adaptations. 2nd edition, p. 437. W. H. Freeman and Company: New York.Google Scholar
Gerasimova, N. G. & Leutskaya, L. K. (1983) On the synthesis of steroids in helminths. Helminlhologia, 20, 81–88.Google Scholar
Gray, C. H. & Bacharach, A. L. (1967) Hormones in Blood. pp. 172–175, Academic Press: New York.Google Scholar
Hieb, W. F. & Rothstein, M. (1968) Sterol requirement of a free-living nematode. Science 160, 778–779.CrossRefGoogle ScholarPubMed
Katz, F. (1967) On a host sex difference in Strongyloides ratti intestinal worm burdens in rats. Proceedings of the Pennsylvania Academy of Science, 41, 30–37.Google Scholar
Kerboeuf, D. (1982) Egg output of Heligmosomoides polygyrus in mice infected once only. Annates de Recherches Vétérinaires, 13, 69–78.Google ScholarPubMed
Keymer, A. E. & Hiorns, R. W. (1986) Faecal egg counts and nematode fecundity: Heligmosomoides polygyrus and laboratory mice. Parasitology, 93, 189–203.CrossRefGoogle ScholarPubMed
Khatoon, H., Wajihullah Baqui, A. & Ansari, J. A. (1986) Endocrinological studies on Setaria cervi infection in white rats: I. Relationship between host sex hormones and metabolic activity of the worm. Helminthologia, 23, 85–91.Google Scholar
Lu, N. C., Newton, C. & Stokstad, E. L. R. (1977) The requirement of sterol precursors in free living nematodes. Nematologica, 23, 57–61.Google Scholar
Morrison, D. D., Vande Waa, E. A. & Bennett, J. L. (1986) Effects of steroids and steroid synthesis inhibitors on the fecundity of Schistosoma mansoni in vitro. Journal of Chemical Ecology, 12, 1901–1908.Google Scholar
Reddington, J. J., Stewart, G. L., Kramar, G. W. & Kramar, M. A. (1981) The effects of host sex and hormones on Trichinella spiralis in the mouse. Journal of Parasitology, 67, 548–555.Google Scholar
Shirazian, D., Childs, J. E., Schiller, E. L. & Hawkes, J. (1986) Mating preference in Schistosoma mansoni in vitro. Journal of Chemical Ecology, 12, 1713–1723.CrossRefGoogle Scholar
Sokal, R. R. & Rohlf, F. J. (1969) Biometry. The principles and practice of statistics in biological research p. 776. W. H. Freeman and Company; San Francisco.Google Scholar
Solomon, G. B. (1966) Development of Nippostrongylus brasiliensis in gonadectomized and hormone treated hamsters. Experimental Parasitology, 18, 374–396.Google Scholar
Swanson, J. A., Falvo, R. & Bone, L. W. (1984) Nippostrongylus brasiliensis: effects of testosterone on reproduction and establishment. International Journal for Parasitology, 14, 241–247.CrossRefGoogle ScholarPubMed
Zar, J. H. (1974) Biostatistical Analysis pp. 109–114. Prentice-Hall Inc., Englewood Cliffs. New Jersey, USA.Google Scholar