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Genetic characterization of three species of Onchocerca at 23 enzyme loci

Published online by Cambridge University Press:  05 June 2009

R. H. Andrews ,
I. Beveridge ,
M. Adams  and
P. R. Baverstock
R. H. Andrews
Affiliation:
The Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, South Australia, Australia5000
I. Beveridge
Affiliation:
Institute of Medical and Veterinary Science, South Australian Department of Agriculture, Frome Road, Adelaide, South Australia, Australia5000
M. Adams
Affiliation:
The Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, South Australia, Australia5000
P. R. Baverstock
Affiliation:
The Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, South Australia, Australia5000
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Abstract

The technique of allozyme electrophoresis was applied to species of Onchocerca from cattle to increase the number of enzyme loci established and therefore provide a genetic basis for a rational species-level taxonomy. Twenty-three enzyme loci were established and provided unequivocal genetic evidence for the taxonomic validity of Onchocerca gibsoni, O. gutturosa and O. lienalis. Furthermore, the diagnostic enzyme markers detected form the basis for identification of life-cycle stages, individuals and species and population structure analyses.

Keywords

Onchocercagenetic characterizationgenetic markersallozyme electrophoresis
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 2 , June 1989 , pp. 87 - 92
Copyright
Copyright © Cambridge University Press 1989

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References

REFERENCES

Agatsuma, T. & Ito, M. (1985) Isozyme study of Onchocerca volvulus and Onchocerca gutturosa in Guatemala. Journal of Parasitology, 71, 370–373.CrossRefGoogle ScholarPubMed
Andrews, R. H., Handman, E., Adams, M., Baverstock, P. R. & Mitchell, G. F. (1988) Genetic characterization of Leishmania isolates at 37 enzyme loci. International Journal for Parasitology, 18, 445–452.CrossRefGoogle ScholarPubMed
Bain, O. (1975) Redescription de cinq espèces d'Onchocerques. Annales de Parasitologie Humaine et Comparée, 50, 763–788.CrossRefGoogle Scholar
Bain, O. (1981) Le genre Onchocerca: hypothèses sur son évolution et clé dichotomique des espèces. Annales de Parasitologie Humaine et Compareé, 56, 503–526.CrossRefGoogle Scholar
Bain, O. & Beveridge, I. (1979) Redescription d'Onchocerca gibsoni C. et J., 1910. Annales de Parasitologie Humaine et Compareé, 54, 69–80.CrossRefGoogle Scholar
Bain, O., Petit, G. & Boulain, B. (1978) Validité des deux espèces Onchocerca lienalis et O. gutturosa, chez les bovins. Annales de Parasitologie Humaine et Compareé, 53, 421–430.CrossRefGoogle Scholar
Baverstock, P. R., Adams, M. & Archer, M. (1984) Electrophoretic resolution of species boundaries in the Sminthopsis murina complex (Dasyuridae). Australian Journal of Zoology, 32, 823–832.CrossRefGoogle Scholar
Baverstock, P. R., Adams, M. & Beveridge, I. (1985) Biochemical differentiation in bile duct cestodes and their marsupial hosts. Molecular and Biochemical Evolution, 2, 321–327.Google ScholarPubMed
Beveridge, I., Kummerow, E. L. & Wilkinson, P. (1980) Observations on Onchocerca gibsoni and nodule development in naturally-infected cattle in Australia. Tropenmedizin und Parasitologie, 31, 75–81.Google ScholarPubMed
Eichler, D. A. & Nelson, G. S. (1971) Studies on Onchocerca gutturosa (Neumann, 1910) and its development in Simulium ornatum (Meigen, 1818). I. Observations on O. gutturosa in cattle in south-east England. Journal of Helminthology, 45, 245–258.CrossRefGoogle Scholar
Flockhart, H. A. (1982) Identification of some Onchocerca spp. of cattle by isozyme analysis. Tropenmendizin und Parasitologie, 33, 51–56.Google Scholar
Flockhart, H. A. & Bianco, A. E. (1985) Differentiation of Onchocerca gutturosa and O. lienalis microfilariae by analysis of their GPI isoenzymes. Journal of Helminthology, 59, 187–189.CrossRefGoogle Scholar
Mattiucci, S., Nascetti, G., Bullini, L., Orecchia, P. & Paggi, L. (1986) Genetic structure of Anisakis physeteris, and its differentiation from the Anisakis simplex complex (Ascarididae: Anisakidae). Parasitology, 93, 383–387.CrossRefGoogle ScholarPubMed
Muller, R. (1979) Identification of Onchocerca In: 17th Symposium of the British Society of Parasitology (editors, Taylor, A. E. R. & Muller, R.) pp. 175–206. Blackwell: Oxford.Google Scholar
Muller, R. (1983) Species recognition in human filarioids. In: Systematics Association Special Volume No. 22 (editors, Stone, A. R., Platt, H. M. & Khalil, L. F.) pp. 339–349. Academic Press: London.Google Scholar
Nadler, S. A. (1987) Biochemical and immunological systematics of some Ascaridoid nematodes: Genetic divergence between congeners. Journal of Parasitology, 73, 811–816.CrossRefGoogle ScholarPubMed
Ottley, M. L. & Moorhouse, D. E. (1978) Bovine onchocerciasis: Aspects of carcase infection. Australian Veterinary Journal, 54, 528–530.CrossRefGoogle Scholar
Ottley, M. L. & Moorhouse, D. E. (1979) Onchocerca (Nematoda: Filarioidea) from Queensland cattle: a redescription of Onchocerca gibsoni (Cleland and Johnston) and O. lienalis (Stiles). Zoologische Anzeiger, 203, 369–377.Google Scholar
Richardson, B. J., Baverstock, P. R. & Adams, M. (1986) Allozyme Electrophoresis: A Handbook for Animal Systematics and Population Studies. Academic Press: Sydney.Google Scholar
Sensabaugh, G. F. (1982) Isozymes in forensic science. In: Isozymes Current Topics in Biological and Medical Research Volume 6 (editor, Liss, A. R.) pp. 247–282.Google Scholar
Thorpe, J. P. (1983) Enzyme variation, genetic distance and evolutionary divergence in relation to levels of taxonomic separation. In: Protein Polymorphism: Adaptive and Taxonomic Significance (editors, Oxford, G. S. & Rollinson, D.) pp. 131–152. Academic Press: London.Google Scholar