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First molecular identification of hydatid tapeworm Echinococcus granulosus sensu lato G6/G7 in Ecuador

Published online by Cambridge University Press:  04 November 2019

D. Ramos-Sarmiento Open the ORCID record for D. Ramos-Sarmiento [Opens in a new window]  and
V. Chiluisa-Utreras Open the ORCID record for V. Chiluisa-Utreras [Opens in a new window]
D. Ramos-Sarmiento*
Affiliation:
Gestión de Investigación, Desarrollo e Innovación, Instituto Nacional de Investigación en Salud Pública, Iquique N14-285 y Yaguachi, Quito, Ecuador
V. Chiluisa-Utreras
Affiliation:
Grupo de Investigación BIOARN, Universidad Politécnica Salesiana, 12 de octubre 2422 y Wilson, Quito, Ecuador
*
Author for correspondence: D. Ramos-Sarmiento, E-mail: [email protected]
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Abstract

Echinococcosis is a zoonotic parasitic illness that can cause significant disabilities, and even death for sick people. The disease is caused by the larval stage of cestodes belonging to the Echinococcus genus. In this study, multiple hydatid cysts were excised from an infected porcine liver. The identification of the parasitic species was made by the morphometric assessment of rostellar hooks and molecular detection of ribosomal DNA extant in protoscoleces of the hydatid sand. Rostellar hooks presented an average length of 27.4 µm by optical microscopy. Parasite DNA were detected in samples of hydatid sediment and positive controls by polymerase chain reaction. In conclusion, Echinococcus granulosus was recognized in samples of porcine hydatid cysts by microscopic observation, and the E. granulosus sensu lato strain E. canadensis G6/G7 was identified by molecular assay.

Keywords

DNA sequenceEcuadorgenus Echinococcushydatidrostellar hooks
Type
Short Communication
Information
Journal of Helminthology , Volume 94 , 2020 , e100
Copyright
Copyright © Cambridge University Press 2019 

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