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Experimental transfer of adult Oesophagostomum dentatum from donor to helminth naive recipient pigs: a methodological study

Published online by Cambridge University Press:  05 June 2009

H. Bjørn ,
A. Roepstorff ,
C. Grøndahl ,
L. Eriksen ,
J. Bjerregaard  and
P. Nansen
H. Bjørn
Affiliation:
Danish Centre for Experimental Parasitology, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
A. Roepstorff
Affiliation:
Danish Centre for Experimental Parasitology, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
C. Grøndahl
Affiliation:
Department of Clinical Studies, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
L. Eriksen
Affiliation:
Department of Clinical Studies, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
J. Bjerregaard
Affiliation:
The Pharmacy, Department of Pharmacology and Pathobiology, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
P. Nansen
Affiliation:
Danish Centre for Experimental Parasitology, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
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Abstract

This study was carried out to compare potential methods of transplanting adult Oesophagostomum dentatum from experimentally infected donor pigs to helminth naive recipient pigs. The following methods were each tested in five pigs: A. Transfer of worms by stomach tube to the gastric ventricle of pigs per os pretreated with 0.5 mg/kg cisapride to increase gastrointestinal peristalsis; B. Transfer by stomach tube to the gastric ventricle of pigs per os pre-treated with cisapride (0.5 mg/kg) and omeprazol 20 mg which blocks hydrochloric acid secretion; C. Surgical transfer of worms to caecum of pigs. Worms for transplantation to pigs were obtained after slaughter of experimentally infected donor pigs and following isolation from the contents of the large intestine, using an agar gel migration technique. A mean of 1054 nematodes were transferred into each recipient pig within 2 hours. Procedures A and B resulted in establishment rates corresponding to only 0.5% and 7.6% of the transferred worms. In contrast, surgical transfer allowed 74.2% of the transplanted worms to be established. In all groups the transplanted worms migrated to the normal predilection site, i.e. the middle part` of the large intestine. More female than male worms established in all groups. It was concluded from this study that surgical transfer was the most reliable of the methods tested for experimental establishment of adult O. dentatum in helminth naive pigs.

Type
Research Papers
Information
Journal of Helminthology , Volume 69 , Issue 4 , December 1995 , pp. 279 - 283
Copyright
Copyright © Cambridge University Press 1995

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