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Electrophoretic identification of larvae and adults of Anisakis (Ascaridida:Anisakidae)

Published online by Cambridge University Press:  05 June 2009

P. Orecchia ,
L. Paggi ,
S. Mattiucci ,
J. W. Smith ,
G. Nascetti  and
L. Bullini
P. Orecchia
Affiliation:
Institute of Parasitology, University of Rome “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, Italy.
L. Paggi
Affiliation:
Institute of Parasitology, University of Rome “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, Italy.
S. Mattiucci
Affiliation:
Institute of Parasitology, University of Rome “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, Italy.
J. W. Smith
Affiliation:
Department of Agriculture and Fisheries for Scotland, Marine Laboratory,P.O. Box 101, Victoria Road, Aberdeen AB9 8DB, Scotland.
G. Nascetti
Affiliation:
Department of Genetics and Molecular Biology, University of Rome “La Sapienza”, Via Lancisi 29, 00161 Rome, Italy.
L. Bullini
Affiliation:
Department of Genetics and Molecular Biology, University of Rome “La Sapienza”, Via Lancisi 29, 00161 Rome, Italy.
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Abstract

The relationships between larvae and adults of Anisakis from the Mediterranean Sea and North-East Atlantic Ocean were analysed by multilocus electrophoresis. The correspondence of type I larvae with the A. simplex complex, including the sibling species A. simplex A and B, and of type II larvae with A. physeteris is confirmed. 19 of the 22 loci studied discriminated between the two larval types. Biochemical keys are given for the electrophoretic identification of A. simplex A, A. simplex B and A. physeteris, at both the larval and adult stages.

Keywords

Anisakis simplexAnisakis physeteriselectrophoresisenzymes
Type
Research Papers
Information
Journal of Helminthology , Volume 60 , Issue 4 , December 1986 , pp. 331 - 339
Copyright
Copyright © Cambridge University Press 1986

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References

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