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Dogs are reservoir hosts for possible transmission of human strongyloidiasis in Thailand: molecular identification and genetic diversity of causative parasite species

Published online by Cambridge University Press:  17 December 2019

O. Sanpool Open the ORCID record for O. Sanpool [Opens in a new window] ,
P.M. Intapan ,
R. Rodpai ,
P. Laoraksawong ,
L. Sadaow ,
S. Tourtip ,
S. Piratae ,
W. Maleewong Open the ORCID record for W. Maleewong [Opens in a new window]  and
T. Thanchomnang Open the ORCID record for T. Thanchomnang [Opens in a new window]
O. Sanpool
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand
P.M. Intapan
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand
R. Rodpai
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand
P. Laoraksawong
Affiliation:
School of Health Science, Sukhothai Thammathirat Open University Pakkret, Nonthaburi11120, Thailand
L. Sadaow
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand
S. Tourtip
Affiliation:
Faculty of Medicine, Mahasarakham University, Maha Sarakham44000, Thailand
S. Piratae
Affiliation:
One Health Research Unit, Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham44000, Thailand
W. Maleewong
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand
T. Thanchomnang*
Affiliation:
Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Faculty of Medicine, Mahasarakham University, Maha Sarakham44000, Thailand
*
Author for correspondence: T. Thanchomnang, E-mail: [email protected]
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Abstract

Human strongyloidiasis is a deleterious gastrointestinal disease mainly caused by Strongyloides stercoralis infection. We aimed to study the possible transmission of S. stercoralis between humans and pet animals. We isolated Strongyloides from humans and domestic dogs in the same rural community in north-east Thailand and compared the nucleotide sequences of derived worms using portions of the mitochondrial cytochrome c oxidase subunit 1 (cox1) and 18S ribosomal RNA (18S rRNA) genes. Twenty-eight sequences from the 18S rRNA gene were obtained from worms derived from humans (n = 23) and dogs (n = 5), and were identical with S. stercoralis sequences (from Thailand, Cambodia, Lao PDR and Myanmar) published in the GenBank database. The 28 cox1 sequences from humans and dogs showed high similarity to each other. The available published cox1 sequences (n = 150), in combination with our 28 sequences, represented 68 haplotypes distributed among four clusters. The 28 samples from the present study represented eight haplotypes including four new haplotypes. Dogs and humans shared the same haplotypes, suggesting the possibility of zoonotic transmission from pet dogs to humans. This is of concern since dogs and humans live in close association with each other.

Keywords

Strongyloides stercoralismolecular identificationhumandogcatzoonotic transmission
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e110
Copyright
Copyright © Cambridge University Press 2019

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References

Arakaki, T, Hasegawa, H, Asato, R, Ikeshiro, T, Kinjo, F, Saito, A and Iwanaga, M (1988) A new method to detect Strongyloides stercoralis from human stool. Japanese Journal of Tropical Medicine and Hygiene 16, 11–17.CrossRefGoogle Scholar
Barratt, JLN, Lane, M, Talundzic, E, et al. (2019) A global genotyping survey of Strongyloides stercoralis and Strongyloides fuelleborni using deep amplicon sequencing. PLoS Neglected Tropical Diseases 13, e0007609.CrossRefGoogle ScholarPubMed
Basso, W, Grandt, LM, Magnenat, AL, Gottstein, B and Campos, M (2019) Strongyloides stercoralis infection in imported and local dogs in Switzerland: from clinics to molecular genetics. Parasitology Research 118, 255–266.CrossRefGoogle ScholarPubMed
Beknazarova, M, Whiley, H and Ross, K (2016) Strongyloidiasis: a disease of socioeconomic disadvantage. International Journal of Environmental Research and Public Health 13, E517.CrossRefGoogle ScholarPubMed
Beknazarova, M, Barratt, JLN, Bradbury, RS, Lane, M, Whiley, H and Ross, K (2019) Detection of classic and cryptic Strongyloides genotypes by deep amplicon sequencing: a preliminary survey of dog and human specimens collected from remote Australian communities. PLoS Neglected Tropical Diseases 13, e0007241.CrossRefGoogle ScholarPubMed
Bisoffi, Z, Buonfrate, D, Montresor, A, et al. (2013) Strongyloides stercoralis: a plea for action. PLoS Neglected Tropical Diseases 7, e2214.CrossRefGoogle Scholar
Deplazes, P, Eckert, J, Mathis, A, von Samson-Himmelstjerna, G and Zahner, H (2016) Parasitology in veterinary medicine. Wageningen, Wageningen Academic Publishers.CrossRefGoogle Scholar
Garcia, L and Bruckner, D (2001) Diagnostic medical parasitology. Washington, DC, American Society for Microbiology.Google Scholar
Grove, DI (1996) Human strongyloidiasis. Advances in Parasitology 38, 251–309.CrossRefGoogle ScholarPubMed
Hall, TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98.Google Scholar
Hasegawa, M, Kishino, H and Yano, T (1985) Dating the human-ape split by a molecular clock of mitochondrial DNA. Journal of Molecular Evolution 22, 160–174.CrossRefGoogle Scholar
Hasegawa, H, Hayashida, S, Ikeda, Y and Sato, H (2009) Hyper-variable regions in 18S rDNA of Strongyloides spp. as markers for species-specific diagnosis. Parasitology Research 104, 869–874.CrossRefGoogle ScholarPubMed
Hasegawa, H, Sato, H, Fujita, S, et al. (2010) Molecular identification of the causative agent of human strongyloidiasis acquired in Tanzania: dispersal and diversity of Strongyloides spp. and their hosts. Parasitology International 59, 407–413.CrossRefGoogle ScholarPubMed
Jaleta, TG, Zhou, S, Bemm, FM, Schär, F, Khieu, V, Muth, S, Odermatt, P, Lok, JB and Streit, A (2017) Different but overlapping populations of Strongyloides stercoralis in dogs and humans-dogs as a possible source for zoonotic strongyloidiasis. PLoS Neglected Tropical Diseases 11, e0005752.CrossRefGoogle ScholarPubMed
Jariwala, S, Redding, L and Hewitt, D (2017) The severely under-recognized public health risk of strongyloidiasis in North American cities-A One Health approach. Zoonoses Public Health 64, 579–588.CrossRefGoogle ScholarPubMed
Laoraksawong, P, Sanpool, O, Rodpai, R, Thanchomnang, T, Kanarkard, W, Maleewong, W, Kraiklang, R and Intapan, PM (2018) Impact of the health education and preventive equipment package (HEPEP) on prevention of Strongyloides stercoralis infection among rural communities in Northeast Thailand: a cluster randomized controlled trial. BMC Public Health 18, 1184.CrossRefGoogle ScholarPubMed
Laymanivong, S, Hangvanthong, B, Insisiengmay, B, et al. (2016) First molecular identification and report of genetic diversity of Strongyloides stercoralis, a current major soil-transmitted helminth in humans from Lao People's Democratic Republic. Parasitology Research 115, 2973–2980.CrossRefGoogle ScholarPubMed
Librado, P and Rozas, J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25, 1451–1452.CrossRefGoogle ScholarPubMed
Nagayasu, E, Aung, MPPTHH, Hortiwakul, T, et al. (2017) A possible origin population of pathogenic intestinal nematodes, Strongyloides stercoralis, unveiled by molecular phylogeny. Scientific Reports 7, 4844.CrossRefGoogle ScholarPubMed
Nutman, TB (2017) Human infection with Strongyloides stercoralis and other related Strongyloides species. Parasitology 144, 263–273.CrossRefGoogle ScholarPubMed
Olsen, A, van Lieshout, L, Marti, H, et al. (2009) Strongyloidiasis–the most neglected of the neglected tropical diseases? Transactions of the Royal Society of Tropical Medicine and Hygiene 103, 967–972.CrossRefGoogle ScholarPubMed
Ramachandran, S, Gam, AA and Neva, FA (1997) Molecular differences between several species of Strongyloides and comparison of selected isolates of S. stercoralis using a polymerase chain reaction-linked restriction fragment length polymorphism approach. The American Journal of Tropical Medicine and Hygiene 56, 61–65.CrossRefGoogle ScholarPubMed
Schär, F, Trostdorf, U, Giardina, F, Khieu, V, Muth, S, Marti, H, Vounatsou, P and Odermatt, P (2013) Strongyloides stercoralis: global distribution and risk factors. PLoS Neglected Tropical Diseases 7, e2288.CrossRefGoogle ScholarPubMed
Takano, Y, Minakami, K, Kodama, S, Matsuo, T and Satozono, I (2009) Cross infection of Strongyloides between humans and dogs in the Amami Islands, Japan. Tropical Medicine and Health 37, 149–152.CrossRefGoogle Scholar
Tamura, K, Stecher, G, Peterson, D, Filipski, A and Kumar, S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 2725–2729.CrossRefGoogle ScholarPubMed
Thamsborg, SM, Ketzis, J, Horii, Y and Matthews, JB (2017) Strongyloides spp. infections of veterinary importance. Parasitology 144, 274–284.CrossRefGoogle ScholarPubMed
Thanchomnang, T, Intapan, PM, Sanpool, O, et al. (2017) First molecular identification and genetic diversity of Strongyloides stercoralis and Strongyloides fuelleborni in human communities having contact with long-tailed macaques in Thailand. Parasitology Research 6, 1917–1923.CrossRefGoogle Scholar
Thompson, JD, Higgins, DG and Gibson, TJ (1994) CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673–4680.CrossRefGoogle Scholar
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