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Diagnosis of schistosomiasis using recombinant fructose-1,6-bisphosphate aldolase from a Formosan strain of Schistosoma japonicum

Published online by Cambridge University Press:  01 September 2009

S.-Y. Peng ,
J.C. Tsaihong ,
P.-C. Fan  and
K.-M. Lee
S.-Y. Peng
Affiliation:
Institute of Tropical Medicine, National Yang-Ming University, No. 155, Sec. 2, Linong St, Beitou District, Taipei City11221, Taiwan
J.C. Tsaihong
Affiliation:
Institute of Tropical Medicine, National Yang-Ming University, No. 155, Sec. 2, Linong St, Beitou District, Taipei City11221, Taiwan
P.-C. Fan*
Affiliation:
Institute of Tropical Medicine, National Yang-Ming University, No. 155, Sec. 2, Linong St, Beitou District, Taipei City11221, Taiwan
K.-M. Lee*
Affiliation:
Institute of Medical Biotechnology, Central Taiwan University of Science and Technology, No.11, Buzih Lane, Beitun District, Taichung City40601, Taiwan
*
*Fax: +886 2 2821 4670, E-mail: [email protected]
†Fax: +886 4 2239 5474, E-mail: [email protected]
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Abstract

Schistosoma japonicum obtained from Taiwan is a zoophilic strain that only infects domestic and small animals. Recombinant fructose-1,6-bisphosphate aldolase (FBPA) derived from this strain was used as an antigen in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of human schistosomiasis. The full-length DNA sequence of FBPA was found to be 1092 bp, encoding a protein of 363 amino acid residues, with a molecular mass of 39.6 kDa. A total of 120 participants were recruited from China and Taiwan to evaluate the diagnostic value of this recombinant protein. In these participants, 34 were found to be infected with S. japonicum, 16 with Ascaris lumbricoides, 15 with hookworm, 13 with Paragonimus westermani and 13 with Clonorchis sinensis, whereas 29 had no ova on faecal examination. Western blot analysis showed that the recombinant FBPA reacts strongly with schistosome ova-positive sera. The sensitivity and specificity of ELISA with FBPA were found to be 85.3% and 93.0%, respectively. These results indicate that FBPA derived from the Formosan strain of S. japonicum can be used for the diagnosis of human schistosomiasis.

Type
Research Papers
Information
Journal of Helminthology , Volume 83 , Issue 3 , September 2009 , pp. 211 - 218
Copyright
Copyright © Cambridge University Press 2008

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References

Chitsulo, L., Loverde, P. & Engels, D. (2004) Schistosomiasis. Nature reviews. Microbiology 2, 12–13.CrossRefGoogle ScholarPubMed
Cross, J.H. (1976) Preliminary observations on the biology of the Indonesian strain of Schistosoma japonicum: animals and oncomelanid snails. Southeast Asian Journal of Tropical Medicine and Public Health 7, 202–207.Google ScholarPubMed
De Jonge, N., Rabello, A.L., Krijger, F.W., Kremsner, P.G., Rocha, R.S., Katz, N. & Deelder, A.M. (1991) Levels of the schistosome circulating anodic and cathodic antigens in serum of schistosomiasis patients from Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 85, 756–759.CrossRefGoogle ScholarPubMed
Doenhoff, M.J., Chiodini, P.L. & Hamilton, J.V. (2004) Specific and sensitive diagnosis of schistosome infection: can it be done with antibodies? Trends in Parasitology 20, 35–39.CrossRefGoogle ScholarPubMed
Duvall, R.H. & DeWitt, W.B. (1967) An improved perfusion technique for recovering adult schistosomes from laboratory animals. American Journal of Tropical Medicine and Hygiene 16, 483–486.CrossRefGoogle ScholarPubMed
El-Dabaa, E., Mei, H., El-Sayed, A., Karim, A.M., Eldesoky, H.M., Fahim, F.A., LoVerde, P.T. & Saber, M.A. (1998) Cloning and characterization of Schistosoma mansoni fructose-1,6-bisphosphate aldolase isoenzyme. Journal of Parasitology 84, 954–960.CrossRefGoogle ScholarPubMed
Galen, R.S. (1980) Predictive value and efficiency of laboratory testing. Pediatric Clinics of North America 27, 861–869.CrossRefGoogle ScholarPubMed
Gatti, S., Bernuzzi, A.M., Bisoffi, Z., Raglio, A., Gulletta, M. & Scaglia, M. (2002) Multicentre study, in patients with imported malaria, on the sensitivity and specificity of a dipstick test (ICT Malaria P.f./P.v.) compared with expert microscopy. Annals of Tropical Medicine and Parasitology 96, 15–18.CrossRefGoogle ScholarPubMed
Genrich, G.L., Guarner, J., Paddock, C.D., Shieh, W.J., Greer, P.W., Barnwell, J.W. & Zaki, S.R. (2007) Fatal malaria infection in travelers: novel immunohistochemical assays for the detection of Plasmodium falciparum in tissues and implications for pathogenesis. American Journal of Tropical Medicine and Hygiene 76, 251–259.CrossRefGoogle ScholarPubMed
Guan, X. & Shi, Y. (1996) Collaborative study on evaluation of immunodiagnostic assays in schistosomiasis japonica by treatment efficacy assessment. Chinese Medical Journal (Engl) 109, 659–664.Google Scholar
Guo, K.W. & Niu, A.O. (2004) Studies on the genetic variation of two mitochondrial DNA molecules of Schistosoma japonicum. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 22, 300–302.Google ScholarPubMed
Hamilton, J.V., Klinkert, M. & Doenhoff, M.J. (1998) Diagnosis of schistosomiasis: antibody detection, with notes on parasitological and antigen detection methods. Parasitology 117 (Suppl.), S41–S57.CrossRefGoogle ScholarPubMed
Harrop, R., Coulson, P.S. & Wilson, R.A. (1999) Characterization, cloning and immunogenicity of antigens released by lung-stage larvae of Schistosoma mansoni. Parasitology 118, 583–594.CrossRefGoogle ScholarPubMed
Hsu, H.F. & Hsu Li, S.Y. (1956) On the infectivity of the Formosan strain of Schistosoma japonicum in Homo sapiens. American Journal of Tropical Medicine and Hygiene 5, 521–528.CrossRefGoogle ScholarPubMed
Hsu, H.F. & Li Hsu, S.Y. (1960) The infectivity of four geographic strains of Schistosoma japonicum in the rhesus monkey. Journal of Parasitology 46, 228.CrossRefGoogle Scholar
Hsu, H.F., Li, S.Y., Wang, C.K., Fan, P.C. & Huang, T.C. (1952) Studies on Schistosomiasis japonica in Formosa. American Journal of Tropical Medicine and Hygiene 1, 287–301.CrossRefGoogle ScholarPubMed
Hsu, S.Y. & Hsu, H.F. (1960) Infectivity of the Philippine strain of Schistosoma japonicum in Oncomelania hupensis, O. formosana and O. nosophora. Journal of Parasitology 46, 793–796.CrossRefGoogle ScholarPubMed
Hsu, S.Y., Davis, J.R. & Hsu, H.F. (1962) Pathology in rhesus monkeys infected with formosan strain of Schistosoma japonicum. Zeitschrift für Tropenmedizin und Parasitologie 13, 341–356.Google ScholarPubMed
Katz, N., Chaves, A. & Pellegrino, J. (1972) A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Revista do Instituto de Medicina Tropical de São Paulo Links 14, 397–400.Google ScholarPubMed
Kulkarni, R.R., Parreira, V.R., Sharif, S. & Prescott, J.F. (2006) Clostridium perfringens antigens recognized by broiler chickens immune to necrotic enteritis. Clinical and Vaccine Immunology 13, 1358–1362.CrossRefGoogle ScholarPubMed
Marsh, J.J. & Lebherz, H.G. (1992) Fructose-bisphosphate aldolases: an evolutionary history. Trends in Biochemical Sciences 17, 110–113.CrossRefGoogle ScholarPubMed
McCarthy, J.S., Wieseman, M., Tropea, J., Kaslow, D., Abraham, D., Lustigman, S., Tuan, R., Guderian, R.H. & Nutman, T.B. (2002) Onchocerca volvulus glycolytic enzyme fructose-1,6-bisphosphate aldolase as a target for a protective immune response in humans. Infection and Immunity 70, 851–858.CrossRefGoogle ScholarPubMed
Moose, J.W., Williams, J.E. & Ota, S. (1963) The susceptibility of white Oncomelania nosophora to infection with the Japanese strain of Schistosoma japonicum. Japanese Journal of Medical Science and Biology 16, 125.CrossRefGoogle ScholarPubMed
Nagano, I., Pei, F., Wu, Z., Wu, J., Cui, H., Boonmars, T. & Takahashi, Y. (2004) Molecular expression of a cysteine proteinase of Clonorchis sinensis and its application to an enzyme-linked immunosorbent assay for immunodiagnosis of clonorchiasis. Clinical and Diagnostic Laboratory Immunology 11, 411–416.Google Scholar
Palm, J.E., Weiland, M.E., Griffiths, W.J., Ljungstrom, I. & Svard, S.G. (2003) Identification of immunoreactive proteins during acute human giardiasis. Journal of Infectious Diseases 187, 1849–1859.CrossRefGoogle ScholarPubMed
Pitarch, A., Diez-Orejas, R., Molero, G., Pardo, M., Sanchez, M., Gil, C. & Nombela, C. (2001) Analysis of the serologic response to systemic Candida albicans infection in a murine model. Proteomics 1, 550–559.3.0.CO;2-W>CrossRefGoogle ScholarPubMed
Qiu, L., Liu, S., Xue, H., Zhang, Y., Li, H., Hu, Y. & He, Y. (1999) SDS–PAGE and EITB analysis of the protein components of different isolates of Schistosoma japonicum in China and Japan. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 17, 367–369.Google ScholarPubMed
Rutter, W.J. (1964) Evolution of aldolase. Federation Proceedings 23, 1248–1257.Google ScholarPubMed
Saliki, J.T. & Lehenbauer, T.W. (2001) Monoclonal antibody-based competitive enzyme-linked immunosorbent assay for detection of morbillivirus antibody in marine mammal sera. Journal of Clinical Microbiology 39, 1877–1881.CrossRefGoogle ScholarPubMed
Schinski, V.D., Clutter, W.C. & Murrell, K.D. (1976) Enzyme- and 125I-labeled anti-immunoglobulin assays in the immunodiagnosis of schistosomiasis. American Journal of Tropical Medicine and Hygiene 25, 824–831.CrossRefGoogle ScholarPubMed
Srivastava, I.K., Schmidt, M., Certa, U., Dobeli, H. & Perrin, L.H. (1990) Specificity and inhibitory activity of antibodies to Plasmodium falciparum aldolase. Journal of Immunology 144, 1497–1503.CrossRefGoogle ScholarPubMed
Tjitra, E., Suprianto, S., Dyer, M., Currie, B.J. & Anstey, N.M. (1999) Field evaluation of the ICT malaria P.f/P.v immunochromatographic test for detection of Plasmodium falciparum and Plasmodium vivax in patients with a presumptive clinical diagnosis of malaria in eastern Indonesia. Journal of Clinical Microbiology 37, 2412–2417.CrossRefGoogle ScholarPubMed
Wanidworanun, C., Nagel, R.L. & Shear, H.L. (1999) Antisense oligonucleotides targeting malarial aldolase inhibit the asexual erythrocytic stages of Plasmodium falciparum. Molecular and Biochemical Parasitology 102, 91–101.CrossRefGoogle ScholarPubMed
Wu, G. (2002) A historical perspective on the immunodiagnosis of schistosomiasis in China. Acta Tropica 82, 193–198.CrossRefGoogle ScholarPubMed
Zhu, Y.C. (2005) Immunodiagnosis and its role in schistosomiasis control in China: a review. Acta Tropica 96, 130–136.CrossRefGoogle Scholar