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Cytochemical observations on cholinergic, serotoninergic and peptidergic neuronal pathways in Cephalochlamys namaquensis

Published online by Cambridge University Press:  05 June 2009

D. M. Mckay ,
D. W. Halton ,
C. F. Johnston ,
C. Shaw  and
I. Fairweather
D. M. Mckay
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology & Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, UK
D. W. Halton*
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology & Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, UK
C. F. Johnston
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology & Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, UK
C. Shaw
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology & Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, UK
I. Fairweather
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of Biology & Biochemistry and Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, UK
*
*Author to whom correspondence should be addressed.
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Abstract

The localization and distribution of cholinergic, serotoninergic (5-HT, serotonin) and peptidergic components of the nervous system of adult Cephalochlamys namaquensis (Cestoda: Pseudophyllidea) have been determined using enzyme histochemical and immunocytochemical techniques interfaced with light and confocal scanning laser microscopy. All three classes of neuroactive substance showed a similar pattern of staining, occurring extensively throughout the central and peripheral nervous systems of the parasite. There were some minor regional differences in staining, suggesting specific roles for certain classes of neurone, and nerve cell bodies were most evident following immunostaining for serotonin. The general overlap in the distribution of staining may be indicative of some co-localization of neurotransmitter and/or neuromodulatory substances.

Keywords

Cephalochlamys namaquensischolinesteraseserotonin (=5-HT)neuropeptidesimmunocytochemistryCestoda
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 3 , September 1992 , pp. 205 - 212
Copyright
Copyright © Cambridge University Press 1992

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References

REFERENCES

Coons, A. H., Leduc, E. G. & Connolly, J. (1955) Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. Journal of Experimental Medicine, 102, 49–60.CrossRefGoogle Scholar
Fairweather, I. & Halton, D. W. (1991) Neuropeptides in platyhelminths. Parasitology, 102, S77–S92.CrossRefGoogle ScholarPubMed
Fairweather, I., Mahendrasingam, S., Johnston, C. F., Halton, D. W. & Shaw, C. (1990a) Peptidergic nerve elements in three developmental stages of the tetraphyllidean tapeworm Trilocularia acanthiaevulgaris. Parasitology Research, 76, 497–508.CrossRefGoogle ScholarPubMed
Fairweather, I., Mahendrasingam, S., Johnston, C. F., Halton, D. W., McCullough, J. S. & Shaw, C. (1990b) An ontogenetic study of the cholinergic and serotoninergic nervous systems in Trilocularia acanthiaevulgaris (Cestoda, Tetraphyllidae). Parasitology Research, 76, 487–496.CrossRefGoogle Scholar
Gomori, G. (1952) Microscopic Histochemistry, Principles and Practice. University of Chicago Press: Chicago.CrossRefGoogle Scholar
Gustafsson, M. K. S. & Reuter, M. (1990) The early brain. Proceedings of the symposium “Invertebrate neurobiology”Abo Akademi University,1989. Acta Academiae Ahoensis, Series B, volume 50, nr 7. Abo Akademis Forlag, Abo Academy Press.Google Scholar
Gustafsson, M. K. S., Wikgren, M. C., Karhi, T. J. & Schot, L. P. C. (1985) Immunocytochemical demonstration of neuropeptides and serotonin in the tapeworm Diphyllobothrium dentriticum. Cell and Tissue Research, 240, 255–260.CrossRefGoogle Scholar
Gustafsson, M. K. S., Lehtonen, M. A. I. & Sundler, F. (1986) Immunocytochemical evidence for the presence of “mammalian” neurohormonal peptides in neurones of the tapeworm Diphyllobothrium dentriticum. Cell and Tissue Research, 243, 41–49.CrossRefGoogle Scholar
Halton, D. W., Fairweather, I., Shaw, C. & Johnston, C. F. (1990) Regulatory peptides in parasitic platyhelminths. Parasitology Today, 6, 284–290.CrossRefGoogle ScholarPubMed
Halton, D. W., Shaw, C., Maule, A. G., Johnston, C. F. & Fairweather, I. (1992) Peptidergic messengers: a new perspective of the nervous system of parasitic platyhelminths. Journal of Parasitology, 78, 179–193.CrossRefGoogle ScholarPubMed
Hariri, M. (1974) Quantitative measurements of endogenous levels of acetylcholine and choline in tetrathyridia of Mesocestoides corti (Cestoda) by means of combined gas chromatography-mass spectrometry. Journal of Parasitology, 60, 227–230.CrossRefGoogle Scholar
Lundberg, J. M. & Hokfelt, T. (1985) Coexistence of peptides and classical neurotransmitters. In: Neurotransmitters in Action. (Editor Bousfield, D.), pp. 104–118. Elsevier Biomedical Press: Amsterdam, New York, Oxford.Google Scholar
Maule, A. G., Shaw, C., Halton, D. W., Thim, L., Johnston, C. F., Fairweather, I. & Buchanan, K. D. (1991) Ncuropeptide F: a novel parasitic flatworm regulatory peptide from Moniezia expansa (Cestoda: Cyclophyllidca). Parasitology, 102, 309–316.CrossRefGoogle Scholar
Mckay, D. M., Fairweather, I., Johnston, C. F., Shaw, C. & Halton, D. W. (1991) Immunocytochemical and radioimmunometrical demonstration of serotonin-and neuropeptide-immunoreactivities in the adult rat tapeworm. Hymenolepis diminuta (Cestoda, Cyclophyllidca). Parasitology, 103, 275–289.CrossRefGoogle Scholar
Stretton, A. O. W., Cowden, C., Sithigorngul, P. & Davis, R. E. (1991) Neuropeptides in the nematode Ascaris suum. Parasitology, 102, S107–S116.CrossRefGoogle ScholarPubMed
Thompson, C. S. & Mettrick, D. F. (1984) Neuromuscular physiology of Hymenolepis diminuta and H. microstoma (Cestoda). Parasitology, 89, 567–578.CrossRefGoogle Scholar
Walker, R. J. & Holden-Dye, L. (1991) Evolutionary aspects of transmitter molecules, their receptors and channels. Parasitology, 102, S7–S29.CrossRefGoogle ScholarPubMed
Ward, S. M., Allen, J. M. & Mckerr, G. (1986) Neuromuscular physiology of Grillotia erinaceus metacestodes (Cestoda: Trypanorhyncha) in vitro. Parasitology, 93, 121–132.CrossRefGoogle Scholar