Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-25T18:38:58.248Z Has data issue: false hasContentIssue false
  • English
  • Français

Cyclic adenosine 3′, 5′-monophosphate in Gastrothylax crumenifer and Explanatum explanatum

Published online by Cambridge University Press:  05 June 2009

H. Ehsan ,
K.A. Rashid ,
W. Van Dongen  and
H.A. Van Onckelen
H. Ehsan
Affiliation:
Department of Biology, University of Antwerp, B-2610 Antwerp, Belgium
K.A. Rashid
Affiliation:
Department of Biochemistry, University of Antwerp, B-2610 Antwerp, Belgium:
W. Van Dongen
Affiliation:
Department of Chemistry, Nucleoside Research and Mass Spectrometry Unit, University of Antwerp, Groenenbogerlaan 171, B-2020 Antwerp, Belgium
H.A. Van Onckelen
Affiliation:
Department of Biology, University of Antwerp, B-2610 Antwerp, Belgium
Get access Rights & Permissions [Opens in a new window]

Abstract

A rapid and reliable method based on immuno-affinity chromatography and preparative high pressure liquid chromatography was used for extraction and purification of cAMP from the two trematode species Gastrothylax crumenifer and Explanatum explanatum. The purified cAMP was quantified by electrospray tandem mass spectrometry in an amount of 44 and 83 picomole/g fresh weight of Gastrothylax crumenifer and Explanatum explanatum respectively.

Type
Research Papers
Information
Journal of Helminthology , Volume 71 , Issue 2 , June 1997 , pp. 147 - 149
Copyright
Copyright © Cambridge University Press 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Brown, E.G. & Newton, R.P. (1992) Analytical procedures for cyclic nucleotides and their associated enzymes in plant tissues. Phytochemical Analysis 3, 1–13.Google Scholar
Jost, J.P. & Rickenberg, H.V. (1971) Cyclic AMP. Annual Review of Biochemistry 40, 741–766.CrossRefGoogle Scholar
Mansour, T.E. (1979) Chemotherapy of parasitic worms: new biochemical strategies. Science 205, 462–469.Google Scholar
Mansour, T.E. & Mansour, J.M. (1977) Phosphodiesterase in the liver fluke, Fasciola hepatica. Biochemical Pharmacology 26, 2325–2330.Google Scholar
Rickenberg, H.V. (1974) Cyclic AMP in prokaryotes. Annual Review of Microbiology 28, 353–369.Google Scholar
Roef, L., Witters, E., Gadeyne, J., Marcussen, J., Newton, R.P. & Van Onckelen, H.A. (1996) Analysis of 3',5'-cAMP and adenyl cyclase activity in higher plants using polyclonal chicken egg yolk antibodies. Analytical Biochemistry 233, 188–196.Google Scholar
Witters, E., Roef, L., Newton, R.P., Dongen, W.V., Esmans, E.L. & Van Onckelen, H.A. (1996) Quanntarion of cyclic nucleotides in biological samples by negative electrospray tandem mass spectrometry coupled to ion suppression liquid chromatography. Rapid Communications in Mass Spectrometry 10, 225–231.Google Scholar
Van Onckelen, H.A., Dupon, M. & De Greef, J.A. (1982) High-performance liquid chromatographic identification and quantitation of cyclic adenosine 3', 5'-monophosphate in higher and lower plants. Physiologia Plantarum 55, 93–97.CrossRefGoogle Scholar