Hostname: page-component-cc8bf7c57-l9twb Total loading time: 0 Render date: 2024-12-12T04:52:39.924Z Has data issue: false hasContentIssue false
  • English
  • Français

Culture and Isolation of Nippostrongylus brasiliensis Infective Larvae for Biochemical Studies*

Published online by Cambridge University Press:  18 November 2009

Peter A. G. Wilson  and
June M. Dick
Peter A. G. Wilson
Affiliation:
Department of Zoology, University of Edinburgh
June M. Dick
Affiliation:
Department of Zoology, University of Edinburgh
Get access Rights & Permissions [Opens in a new window]

Extract

Methods for isolating the various stages of parasite life-cycles in large amounts and in a clean condition for biochemical and immunological studies are urgently needed. Among the parasitic nematodes the methods so far developed mainly concern the adult worm, though the eggs of some of the Ascaroidea are notable exceptions. This paper describes adaptations of one of the traditional methods of culturing Nippostrongylus infective larvae which satisfy the needs of biochemical and immunological studies.

Type
Research Papers
Information
Journal of Helminthology , Volume 38 , Issue 3-4 , December 1964 , pp. 399 - 404
Copyright
Copyright © Cambridge University Press 1964

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barakat, M. R., 1951—“A new procedure for the cultivation of nematode parasites.” J. Egypt, med. Ass., 34, 323–326.Google ScholarPubMed
Hale, L. J., 1958.—Biological Laboratory Data. Methucn, London.Google Scholar
Haley, A. J., 1962—“Biology of the rat nematode, Nipposlrotigylus brasilicttsis (Travassos, 1914). II Preparasitic stages and development in the laboratory rat.” J. Parasit., 48, 13–23.CrossRefGoogle Scholar
Leigh, L. C, 1956.—“Methods for the culture and collection of Nipposlrongylus muris.” J. Ittst. Sci. Technol., 2, 21–23.Google Scholar
Mokrasch, L. C, 1954.—“Analysis of hexose phosphates and sugar mixtures with the anthrone reagent.” J. biol. Chenu, 208, 55–59.CrossRefGoogle ScholarPubMed
Schwabe, C. W., 1957.—“Observations on the respiration of free-living and parasitic Nipposlrongylus muris larvae.” Am. J. Hyg., 65, 325–337.Google ScholarPubMed