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Comparative spectral, electrophoretic and isoelectric properties of trematode haemoglobins

Published online by Cambridge University Press:  05 June 2009

K. A. Rashid ,
M. Haque  and
A. H. Siddiqi
K. A. Rashid
Affiliation:
Section of Parasitology, Department of Zoology, Aligrah Muslim University, Aligrah 202002, India
M. Haque
Affiliation:
Section of Parasitology, Department of Zoology, Aligrah Muslim University, Aligrah 202002, India
A. H. Siddiqi
Affiliation:
Section of Parasitology, Department of Zoology, Aligrah Muslim University, Aligrah 202002, India
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Abstract

A comparative studs of the spectral, electrophoretic and isoelectric properties of the haemoglobins of three trematodes. Paramphistomum epiclitum. Gigantocotyle explanatum and Gastrothylax erumenifer was carried out. A high absorption in the beta band region indicates that trematode haemoglobins have high oxygen affinities. Electrophoretic mobilities of all trematode and their host haemoglobins were different. The isoelectric points of trematode haemoglobins were found to focus in the acidic range except that of G. crumenifer haemoglobin I. which focused at an alkaline pH.

Keywords

haemoglobinTrematodaGastrothylax crumeniferGigantocotyle explanatumParamphistomum epiclitumbiochemistry
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 3 , September 1993 , pp. 200 - 204
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Cain, G. D. (1969) Purification and properties of hemoglobin from Fasciolopsis buski. Journal of Parasitology, 55, 311–320.CrossRefGoogle ScholarPubMed
Halton, D. W. (1967) Observations on nutrition of digenetic trematodes. Parasitology, 57, 639–660.Google Scholar
Haider, S. A. & Siddiqi, A. H. (1976) Spectrophotometric analysis of haemoglobins of some digenetic trematodes and their hosts. Journal of Helminthology, 50, 259–266.CrossRefGoogle ScholarPubMed
Haider, S. A. & Siddiqi, A. H. (1977) Alkali denaturation of oxyhaemoglobins of some digenetic trematodes and their hosts. Journal of Helminthology, 51, 373–378.CrossRefGoogle ScholarPubMed
Haque, M., Rashid, K. A., Stern, M. S., Sharma, P. K., Siddiqi, A. H., Vinogradov, S. N. & Walz, D. A. (1992) Comparison of the hemoglobins of the Platyhelminths Gastrothylax crumenifer and Paramphistomum epiclitum (Trematoda: Paramphistomatidae) Comparative Biochemistry and Physiology, 101B, 673–676.Google Scholar
Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 277, 680–685.CrossRefGoogle Scholar
Lee, D. J. & Smith, M. H. (1969) Hemoglobins of parasitic animals. Experimental Parasitology, 16, 392–424.CrossRefGoogle Scholar
Lutz, P. L. & Siddiqi, A. H. (1967) Comparison of hemoglobins of Fasciola gigantica (Trematoda: Digenea) and its host. Experimental Parasitology, 20, 83–87.CrossRefGoogle ScholarPubMed
Prosser, C. L. (1973) Comparative Animal Physiology. 3rd edition. Saunders, Philadelphia, Pennsylvania.Google Scholar
Siddiqui, J. & Siddiqi, A. H. (1987) Purification and properties of haemoglobin from Gastrothylax crumenifer. (Trematoda: Paramphistomatidae) Journal of Helminthology, 61, 146–150.Google Scholar
Smyth, J. D. & Halton, D. W. (1983) The Physiology of Trematodes. 2nd edition, 68–69, Cambridge University Press, Cambridge.Google Scholar
Todd, J. R. & Ross, J. R. (1966) Origin of hemoglobin in the cecal contents of Fasciola hepatica. Experimental Parasitology, 19, 151–154.CrossRefGoogle ScholarPubMed
Tuchschmid, P. E., Kunz, P. A. & Wilson, K. J. (1978) Isolation and characterization of the hemoglobin from the lanceolate fluke Dicrocoelium, dendriticum. European Journal of Biochemistry, 88, 387–394.CrossRefGoogle ScholarPubMed
Vandergon, T. L., Noblet, G. P. & Colacino, J. M. (1988) Identification and origin of hemoglobin in a gymnophallid metacercaria (Trematoda: Digenea), a symbiote in the marine polychaete Amphitrite ornata (Annelida: Terebellidae). Biological Bulletin, 174, 172–180.Google Scholar
Weishman, I. E. & Cain, G. D. (1973) Extraction and characterization of tetrapyrrole pigments from intestinal cells of Ascaris lumbricoides. International Journal of Biochemistry, 4, 349–355.CrossRefGoogle Scholar