Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-08T09:18:50.915Z Has data issue: false hasContentIssue false
  • English
  • Français

Characterization of Fasciola hepatica redial generations by morphometry and chaetotaxy under experimental conditions

Published online by Cambridge University Press:  05 June 2009

D. Augot ,
D. Rondelaud ,
G. Dreyfuss ,
J. Cabaret ,
C. Bayssade-Dufour  and
J.L. Albaret
D. Augot
Affiliation:
Laboratoire d'Histopathologie Parasitaire, Faculté de Médecine Laboratoire de Parasitologie, Faculté de Pharmacie, 2, rue du Docteur Raymond Marcland, 87025 Limoges Cedex, France
D. Rondelaud*
Affiliation:
Laboratoire d'Histopathologie Parasitaire, Faculté de Médecine
G. Dreyfuss
Affiliation:
Laboratoire de Parasitologie, Faculté de Pharmacie, 2, rue du Docteur Raymond Marcland, 87025 Limoges Cedex, France
J. Cabaret
Affiliation:
Station de Pathologie Aviaire et de Parasitologie, Institut National de la Recherche Agronomique, 37380 Nouzilly Cedex, France
C. Bayssade-Dufour
Affiliation:
Laboratoire de Biologie Parasitaire, Muséum National d'Histoire Naturelle, 61, rue Buffon, 75231 Paris Cedex 5, France
J.L. Albaret
Affiliation:
Laboratoire de Biologie Parasitaire, Muséum National d'Histoire Naturelle, 61, rue Buffon, 75231 Paris Cedex 5, France
*
*Author for correspondence. Fax: 33 5 55 43 58 93 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Morphometric and chaetotactic studies were carried out on the body and cephalic regions of the rediae of Fasciola hepatica (Trematoda: Fasciolidae) in order to precisely identify the different redial generations of this trematode in Lymnaea truncatula under experimental infection. At day 49 post-exposure at 20°C, the length of the redia was significantly higher in the first group of the first generation (R1a) compared with successive generations, R1b, R2a and R2b/R3a. The width of the body was similar in the R1a, R1b, and R2a rediae, but was significantly lower in the R2b/R3a groups. The intrapharyngeal cavity of R1a rediae was significantly wider compared with the R1b, R2a, and R2b/R3a groups, whereas the pharyngeal wall was significantly thicker in the R2b/R3a rediae compared with the R1b and R2a groups. Four other measurements, namely the maximum length and width of the pharynx, diameter of the mouth, and width of intestine, also showed significant variations in relation to pharyngeal morphology and age of infection. Discriminant analysis based on these measurements demonstrated that 98% of the rediae were readily categorized into the four groups identified. The number of perioral sensillae ranged from 126 to 160 but a significant difference was only noted between the mean values of the first generation and those of the group R2b/R3a. From these parameters, the maximum width of the pharyngeal lumen was found to be the best characteristic in the identification of the redial generations.

Type
Research Papers
Information
Journal of Helminthology , Volume 72 , Issue 3 , September 1998 , pp. 193 - 198
Copyright
Copyright © Cambridge University Press 1998

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Augot, D., Abrous, M., Rondelaud, D., Dreyfuss, G., Rakotondravao, & Cabaret, J. (1995) Les générations rédiennes de Fasciola gigantica Cobbold. Leur caractérisation par l'étude morphologique et la chétotaxie de la région pharyngienne. Bulletin de la Société Francaise de Parasitologie 13, 173–180.Google Scholar
Bayssade-Dufour, C., Albaret, J.L., Samnaliev, P., Cassone, J. & Dimitrov, V. (1980) Les structures argyrophiles tégumentaires des stades larvaires (miracidium, rédie, cercaire) de Fasciola hepatica. Comparaison avec F. gigantica. Annales de Parasitologie Humaine et Comparée, 55, 553–564.CrossRefGoogle ScholarPubMed
Combes, C., Bayssade-Dufour, C. & Cassone, J. (1976) Sur l'imprégnation et le montage des cercaires pour l'étude chétotaxique. Annales de Parasitologie Humaine et Comparée 51, 399–400.CrossRefGoogle Scholar
Dimitrov, V. & Samnaliev, P. (1990) Argentophilic structures of rediae of trematode species from family Paramphisto-midae and family Fasciolidae. Helminthologiya 28, 30–35. (In Bulgarian.)Google Scholar
Dinnik, J.A. & Dinnik, N.N. (1956) Observations on the succession of redial generations of Fasciola gigantica Cobbold, in a snail host. Zeitschrift für Tropenmedizin und Parasitologie 7, 397–419.Google Scholar
Lebart, L., Morineau, A. & Warwick, K. (1984) Multivariate descriptive statistical analysis. New York, J. Wiley.Google Scholar
Manga-Gonzalez, Y., Gonzalez-Lanza, C. & Kanev, I. (1994) Lymnaea truncatula, the intermediate host of some Plagiorchidae and Notocotylidae species in Leon, NW Spain. Journal of Helminthology 68, 134–141.CrossRefGoogle Scholar
Ollerenshaw, C.B. (1971) Some observations on the epidemiology of fascioliasis in relation to the timing of molluscicide application in the control of the disease. Veterinary Record 88, 152–164.CrossRefGoogle Scholar
Préveraud-Sindou, M. & Rondelaud, D. (1995) Localization and outcome of Fasciola hepatica sporocysts in Lymnaea truncatula subjected to mono- or plurimiracidial exposures. Parasitology Research 81, 265–267.CrossRefGoogle ScholarPubMed
Rakotondravao, , Moukrim, A., Hourdin, P. & Rondelaud, D. (1992) Redial generations of Fasciola gigantica in the pulmonate snail Lymnaea truncatula. Journal of Helminthology 66, 159–166.CrossRefGoogle ScholarPubMed
Rondelaud, D. (1974) L'évolution des rédies de Fasciola hepatica L. chez Galba truncatula Müller en Limousin. Revue de Médecine Vétérinaire 125, 237–250.Google Scholar
Rondelaud, D. (1978) Contribution à l'étude écologique et éthologique de Lymnaea (Galba) truncatula Müller, vecteur de Fasciola hepatica L. Recherche de moyens de lutte biologique en Limousin. Doctorate Thesis, Limoges, France, n°4.Google Scholar
Rondelaud, D. & Barthe, D. (1978) Arguments et propositions pour une nouvelle interprétation de l'évolution de Fasciola hepatica L. dans Lymnaea (Galba) truncatula Müller. Annales de Parasitologie Humaine et Comparée 53, 201–213.CrossRefGoogle ScholarPubMed
Rondelaud, D. & Barthe, D. (1982) Les générations rédiennes de Fasciola hepatica L. chez Lymnaea truncatula Müller. A propos des effets de plusieurs facteurs. Annales de Parasitologie Humaine et Comparée 57, 245–262.CrossRefGoogle Scholar
Rondelaud, D. & Barthe, D. (1987) Fasciola hepatica L.: étude de la productivité d'un sporocyste en fonction de la taille de Lymnaea truncatula Müller. Parasitology Research 74, 155–160.CrossRefGoogle Scholar
Stat-Itcf, (1988) Manuel d'utilisation. Institut Technique des Céréales et des Fourrages, Service des Etudes Statistiques, Boigneville, France.Google Scholar
Szmidt-Adjidé, V., Rondelaud, D. & Dreyfuss, G. (1994) Premières données sur l'infestation naturelle de Lymnaea truncatula Müller par Paramphistomum daubneyi Dinnik dans le département de la Haute-Vienne. Bulletin de la Société Française de Parasitologie 12, 183–188.Google Scholar
Thomas, A.P. (1883) The natural history of the liver fluke and the prevention of rot. Journal of the Royal Agricultural Society of England 19, 276–305.Google Scholar
Vala, J.C. (1973) Etude écologique du parasitisme des mollusques de la Mosson, hôtes intermédiaires de Trématodes. Doctorate Thesis, Parasitology, Montpellier, France, n°1455.Google Scholar