Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-24T18:05:58.089Z Has data issue: false hasContentIssue false
  • English
  • Français

Abdominal angiostrongyliasis: pathologic findings in Swiss mice infected with different doses of Angiostrongylus costaricensis

Published online by Cambridge University Press:  07 July 2020

C.C. Hermes ,
E. Benvegnú ,
M.M. Costa ,
R. Rodriguez  and
M.I.B. Vieira Open the ORCID record for M.I.B. Vieira [Opens in a new window]
C.C. Hermes
Affiliation:
Programa de Pós-graduação em Bioexperimentação, Universidade de Passo Fundo – UPF, Passo Fundo, RS, Brazil
E. Benvegnú
Affiliation:
Programa de Pós-graduação em Bioexperimentação, Universidade de Passo Fundo – UPF, Passo Fundo, RS, Brazil
M.M. Costa
Affiliation:
Programa de Pós-graduação em Bioexperimentação, Universidade de Passo Fundo – UPF, Passo Fundo, RS, Brazil
R. Rodriguez
Affiliation:
Instituto de Patologia de Passo Fundo, Passo Fundo, RS, Brazil
M.I.B. Vieira*
Affiliation:
Programa de Pós-graduação em Bioexperimentação, Universidade de Passo Fundo – UPF, Passo Fundo, RS, Brazil
*
Author for correspondence: M.I.B. Vieira, E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Abdominal angiostrongyliasis is caused by Angiostrongylus costaricensis, the definitive and intermediate hosts of which are wild rodents and terrestrial molluscs, respectively. Humans are accidental hosts and can be infected by ingesting the third-stage (infective) larvae (L3). It remains unclear whether the number of L3 inoculated is related to lesion severity. Our aim was to analyse histopathological alterations in Swiss mice infected with different doses of A. costaricensis. Thirty-two mice were randomly divided into four groups (n = 8/group): uninfected, control mice; mice infected with a low dose (five L3); mice infected with an intermediate dose (15 L3); and mice infected with a high dose (30 L3). The frequency of intestinal thrombi, splenitis, eggs/larvae, hepatic infarction and acute pancreatitis differed among the groups, the last being considered a significant finding. We conclude that different infective doses alter the histopathological aspects of the infection in Swiss mice, those aspects being more pronounced at medium and high doses, with no effect on the development of the disease. This experimental model shows that the parasite life cycle can be maintained in Swiss mice through the inoculation of a low dose (five L3).

Keywords

Abdominal angiostrongyliasisinfective dosehistopathologypancreatitis
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e169
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agostini, AA, Peixoto, A, Caleffi, AL, Dexhaimer, A and Camargo, RR (1983) Angiostrongilíase abdominal: Três casos observados no Rio Grande do Sul. Revista AMRIGS 27, 200–203.Google Scholar
Bender, AL, Maurer, RL, da Silva, MCF, Bem, R, Terraciano, PB, da Silva, ACA and Graeff Teixeira, C (2003) Ovos e órgãos reprodutores de fêmeas de Angiostrongylus costaricensis são reconhecidos mais intensamente por soros humanos de fase aguda na angiostrongilíase abdominal. Revista da Sociedade Brasileira de Medicina Tropical 36, 449–454.CrossRefGoogle Scholar
Da Silva, ACA, Graeff-Teixeira, C and Zaha, A (2003) Diagnosis of abdominal angiostrongyliasis by PCR from sera of patients. Revista do Instituto de Medicina Tropical de São Paulo 45(5), 295–297.CrossRefGoogle ScholarPubMed
Economou, M and Zissis, M (2000) Infectious cases of acute pancreatitis. Annals of Gastroenterology 13, 98–101.Google Scholar
Fan, CK, Lin, YH, Du, WY and Su, KE (2003) Infectivity and pathogenicity of 14-month-cultured embryonated eggs of Toxocara canis in mice. Veterinary Parasitology 113(2), 145–55.CrossRefGoogle ScholarPubMed
Garrido, CT, Morassutti, AL, Barradas, JRS and Graeff-Teixeira, C (2017) Evaluating host–parasite co-adaptation relationships involving Angiostrongylus costaricensis. Journal of Helminthology 93(1), 76–80.CrossRefGoogle ScholarPubMed
Graeff-Teixeira, C, Camillo-Coura, L and Lenzi, HL (1987) Abdominal angiostrongyliasis – an under diagnosed disease. Memória do Instituto Oswaldo Cruz 82, 353–354.CrossRefGoogle ScholarPubMed
Graeff-Teixeira, C, Camillo-Coura, L and Lenzi, HL (1991) Histopathological criteria for the diagnosis of abdominal angiostrongyliasis. Parasitology Research 77, 606–611.CrossRefGoogle Scholar
Graeff-Teixeira, C, Agostini, AA, Camillo-Coura, L and Ferreira-da-Cruz, MF (1997) Soroepidemiology of abdominal angiostrongyliasis: the standardization of an immunoenzymatic assay and prevalence of antibodies in two localities in Southern Brazil. Tropical Medicine e International Health 2, 254–260.CrossRefGoogle Scholar
Gundamaraju, R and Vemuri, RC (2014) Opisthorchis viverrini (liver fluke) as the lot of baleful parasite of tropical region-A replete synopsis. Asian Pacific Journal of Tropical Medicine 4(1), 61–66.CrossRefGoogle Scholar
Hermes, CC, Benvegnú, E, Costa, MM, Rodriguez, R and Vieira, MIB (2018) Shedding of Angiostrongylus costaricensis larvae in the faeces of Swiss mice experimentally infected with different infective doses. Journal of Helminthology 24, 1–4.Google Scholar
Iabuki, K and Montenegro, MR (1979) Apendicite por Angiostrongylus costaricensis. Apresentação de um caso. Revista do Instituto Médico Tropica de São Paulo 21, 33–36.Google Scholar
Ishih, A and Nishimura, M (1997) Differential responses of SM/J and A/J mice to experimental Angiostrongylus costaricensis infection. Journal of Parasitology 27, 1411–1414.Google Scholar
Ishih, AI and Sano, M (1989) Strain dependent differences in susceptibility of mice to experimental Angiostrongylus costaricensis infection. Journal of Helminthology 63, 302–306.CrossRefGoogle Scholar
Junqueira, LC and Carneiro, J (1995) Histologia Básica. 8th edn.Rio de Janeiro, Guanabara Koogan.Google Scholar
Khuroo, MS, Zargar, SA, Yattoo, GN, Koul, P, Khan, BA, Dar, MY and Alai, MS (1992) Ascaris-induced acute pancreatitis. British Journal of Surgery 79(12), 1335–1338.CrossRefGoogle ScholarPubMed
Liu, YM, Bair, MJ, Chang, WH, Lin, SC and Chan, YJ (2005) Acute pancreatitis caused by tapeworm in the biliary tract. The American Journal of Tropical Medicine and Hygiene 73(2), 377–380.CrossRefGoogle ScholarPubMed
Mentz, MB and Graeff-Teixeira, C (2003) Drug trials for treatment of human angiostrongyliasis. Revista do Instituto Médico Tropical de São Paulo 45, 179–184.CrossRefGoogle ScholarPubMed
Morera, P (1985) Abdominal angiostrongyliasis: a problem of public health. Parasitology 1, 173–175.Google ScholarPubMed
Morera, P and Cespedes, R (1971) Angiostrongylus costaricensis n. sp. (Nematoda: Metastrongyloidea), a new lungworm occurring in man in Costa Rica. Revista de Biología Tropical 18, 173–185.Google Scholar
Ozmen, MM, Moran, M, Karakahya, M and Coskun, F (2005) Recurrent acute pancreatitis due to a hydatid cyst of the pancreatic head: a case report and review of the literature. Journal of Pathology 6(4), 354–358.Google ScholarPubMed
Rodriguez, R, Dequi, RM, Peruzzo, L, Mesquita, PM, Garcia, E and Fornari, F (2008) Abdominal angiostrongyliasis: report of two cases with different clinical presentations. Revista do Instituto de Medicina Tropical de São Paulo 50, 339–341.CrossRefGoogle ScholarPubMed
Rodriguez, R, Porto, SM, Ferrari, RS, Marcolan, AM, da Silva, ACA, Graeff-Teixeira, C and Fornari, F (2011) Outcomes in mice with abdominal angiostrongyliasis treated with enoxaparin. Parasitology Research 9, 787–792.CrossRefGoogle Scholar
Rodriguez, R, Silva, ACA, Müller, CA, Alves, SL, Graeff-Teixeira, C and Fornari, F (2014) PCR for the diagnosis of abdominal angiostrongyliasis in formalin-fixed paraffin-embedded human tissue. PLoS ONE 9, 1–5.CrossRefGoogle Scholar
Sandri, ASS, Rodriguez, R, Costa, MM, Porto, SM, Schwingell, D and Vieira, MIB (2017) High-dose enoxaparin in the treatment of abdominal angiostrongyliasis in Swiss mice. Journal of Helminthology 92(6), 662–667.CrossRefGoogle ScholarPubMed
Sierra, E and Morera, P (1972) Angiostrongilosis abdominal. Primer caso humano encontrado en Honduras (Hospital Evangélico de Siguatepeque). Acta Médica 15, 95–99.Google Scholar
Singh, H, Jain, D and Kiran, B (2016) Acute pancreatitis complicating hepatitis A virus infection: a case report. Eastern Journal of Medicine 1(2), 71–72.Google Scholar
Veerappan, A, Siegel, JH, Podany, J, Prudente, R and Gelb, A (1991) Fasciola hepatica pancreatitis: endoscopic extraction of live parasites. Gastrointestinal Endoscopy 37(4), 473–475.CrossRefGoogle ScholarPubMed