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5′-Nucleotidase activity and substrate affinity in digenetic trematodes

Published online by Cambridge University Press:  18 November 2009

Afzal A. Siddiqui  and
Wajih A. Nizami
Afzal A. Siddiqui
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh-202001 (U.P.), India.
Wajih A. Nizami
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh-202001 (U.P.), India.
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Abstract

5′-nucleotidases of eight species of digenetic trematodes were studied using five different substrates. All species showed the following preferential order of substrate affinity; AMP>CMP>GMP>TMP>UMP. It was observed that different species occupying similar habitats possessed closely related levels of enzyme activities. The function of 5′-nucleotidases in trematodes is also suggested.

Type
Research Note
Information
Journal of Helminthology , Volume 59 , Issue 3 , September 1985 , pp. 263 - 266
Copyright
Copyright © Cambridge University Press 1985

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References

REFERENCES

Gerlach, U. & Hiby, W. (1974) '-Nucleotidase. In: Methods of Enzymatic Analysis. 2nd Edition (editor, Bergmeyer, H. U.) pp. 871–875. Academic Press: New York.CrossRefGoogle Scholar
Lowry, O. H., Rosebrough, N., Farr, A. & Randall, R. (1951) Protein measurement with folin phenol reagent. Journal of Biological Chemistry, 193, 266–275.Google Scholar
Marinetti, G. V. (1962) Chromatographic separation, identification, and analysis of phosphatides. Journal ofLipid Research, 3, 1–20.Google Scholar
Nizami, W. A., Siddiqi, A. H., Yusufi, n. K. (1975). Non-specific alkaline phosphomonoesterases of eight species of digenetic trematodes. Journal of Helminthology, 49, 281–287.CrossRefGoogle ScholarPubMed
Nollen, P. M.Restaino, A. L., Alberico, R. A. (1973) Gorgoderina attenuate: Uptake and incorporation of tyrosine, thymidine and adenosine. Experimental Parasitology, 33, 468–476.CrossRefGoogle Scholar
Nollen, P. M.Pyne, J. L., Bajt, J. E. (1974) Megalodiscus temperatus: absorption and incorporation of tritiated tyrosine, thymidine and adenosine. Experimental Parasitology, 35, 132–140.CrossRefGoogle ScholarPubMed
Senft, A. W., Seyft, D. G. & Miech, R. P. (1973) Pathways of nucleotide metabolism in Schistosoma mansoni II. Deposition of adenosine by whole worms. Biochemical Pharmacology, 22, 437–447.Google Scholar
Sharma, P. N. (1976) Histochemical studies on the distribution of alkaline phosphatase, acid phosphatase, 5'-nucleotidase and ATPase in various reproductive tissues of certain digenetic trematodes. Zeitschrift fiir Parasitenkunde, 49, 223–231.Google Scholar
Vorbrodt, A. (1974) Cytochemistry of nuclear enzymes. In: The cell nucleus (editor, Buch, H.) 327. Academic Press: New York.Google Scholar