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Effect of finite sampling time on estimation of Brownian fluctuation
Published online by Cambridge University Press: 12 February 2015
Abstract
We present a study of the effect of finite detector integration/exposure time $E$, in relation to interrogation time interval ${\rm\Delta}t$, on analysis of Brownian motion of small particles using numerical simulation of the Langevin equation for both free diffusion and hindered diffusion near a solid wall. The simulation result for free diffusion recovers the known scaling law for the dependence of estimated diffusion coefficient on $E/{\rm\Delta}t$, i.e. for $0\leqslant E/{\rm\Delta}t\leqslant 1$ the estimated diffusion coefficient scales linearly as $1-(E/{\rm\Delta}t)/3$. Extending the analysis to the parameter range $E/{\rm\Delta}t\geqslant 1$, we find a new nonlinear scaling behaviour given by $(E/{\rm\Delta}t)^{-1}[1-((E/{\rm\Delta}t)^{-1})/3]$, for which we also provide an exact analytical solution. The simulation of near-wall diffusion shows that hindered diffusion of particles parallel to a solid wall, when normalized appropriately, follows with a high degree of accuracy the same form of scaling laws given above for free diffusion. Specifically, the scaling laws in this case are well represented by $1-((1+{\it\epsilon})(E/{\rm\Delta}t))/3$, for $E/{\rm\Delta}t\leqslant 1$, and $(E/{\rm\Delta}t)^{-1}[1-((1+{\it\epsilon})(E/{\rm\Delta}t)^{-1})/3]$, for $E/{\rm\Delta}t\geqslant 1$, where the small parameter ${\it\epsilon}$ depends on the size of the near-wall domain used in the estimation of the diffusion coefficient and value of $E$. For the range of parameters reported in the literature, we estimate ${\it\epsilon}<0.03$. The near-wall simulations also show a bias in the estimated diffusion coefficient parallel to the wall even in the limit $E=0$, indicating an overestimation which increases with increasing time delay ${\rm\Delta}t$. This diffusion-induced overestimation is caused by the same underlying mechanism responsible for the previously reported overestimation of mean velocity in near-wall velocimetry.
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