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Preventive effects of kefir on colon tumor development in Wistar rats: gut microbiota critical role

Published online by Cambridge University Press:  27 January 2025

Poliana Guiomar de Almeida Brasiel Open the ORCID record for Poliana Guiomar de Almeida Brasiel [Opens in a new window] ,
Julliane Dutra Medeiros ,
Thaís Costa de Almeida ,
Claudio Teodoro de Souza ,
Gabriela de Cássia Ávila Alpino ,
Alessandra Barbosa Ferreira Machado  and
Sheila Cristina Potente Dutra Luquetti
Poliana Guiomar de Almeida Brasiel
Affiliation:
Department of Nutrition, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Julliane Dutra Medeiros
Affiliation:
Department of Biology, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Thaís Costa de Almeida
Affiliation:
Department of Nutrition, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Claudio Teodoro de Souza
Affiliation:
Department of Clinical Medicine, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Gabriela de Cássia Ávila Alpino
Affiliation:
Department of Nutrition, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Alessandra Barbosa Ferreira Machado
Affiliation:
Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, Minas Gerais, Brazil
Sheila Cristina Potente Dutra Luquetti*
Affiliation:
Department of Nutrition, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
*
Corresponding author: Sheila Cristina Potente Dutra Luquetti; Email: [email protected]
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Abstract

To clarify the effects of kefir in critical periods of development in adult diseases, we study the effects of kefir intake during early life on gut microbiota and prevention of colorectal carcinogenesis in adulthood. Lactating Wistar rats were divided into three groups: control (C), kefir lactation (KL), and kefir puberty (KP) groups. The C and KP groups received 1 mL of water/day; KL dams received kefir milk daily (108 CFU/mL) during lactation. After weaning (postnatal day 21), KP pups received kefir treatment until 60 days. At 67 days old, colorectal carcinogenesis was induced through intraperitoneal injection of 1, 2-dimethylhydrazine. The gut microbiota composition were analyzed by 16S rRNA gene sequencing and DESeq2 (differential abundance method), revealing significant differences in bacterial abundances between the kefir consumption periods. Maternal kefir intake strong anticancer power, suppressed tumors in adult offspring and reduced the relative risk of offspring tumor development. The gut microbiota in cecal samples of the KL group was enriched with Lactobacillus, Romboutsia, and Blautia. In contrast, control animals were enriched with Acinetobacter. The administration of kefir during critical periods of development, with emphasis on lactation, affected the gut microbial community structure to promote host benefits. Pearson analysis indicated positive correlation between tumor number with IL-1 levels. Therefore, the probiotic fermented food intake in early life may be effective as chemopreventive potential against colon tumor development, especially in lactation period.

Keywords

Microbiotacolorectal neoplasmskefirprobioticsdevelopmental programming
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 16 , 2025 , e5
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Copyright
© The Author(s), 2025. Published by Cambridge University Press in association with The International Society for Developmental Origins of Health and Disease (DOHaD)

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