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Maternal protein restriction before pregnancy reduces offspring early body mass and affects glucose metabolism in C57BL/6JBom mice

Published online by Cambridge University Press:  21 May 2012

A. Dudele ,
S. Lund ,
N. Jessen ,
G. Wegener ,
G. Winther ,
J. Elnif ,
S. Frische ,
T. Wang  and
D. Mayntz
A. Dudele*
Affiliation:
Department of Bioscience, Zoophysiology, University of Aarhus, Aarhus C, Denmark
S. Lund
Affiliation:
Medical Research Laboratory and Medical Department M (Endocrinology and Diabetes), Aarhus University Hospital, Aarhus C, Denmark
N. Jessen
Affiliation:
Medical Research Laboratory and Medical Department M (Endocrinology and Diabetes), Aarhus University Hospital, Aarhus C, Denmark
G. Wegener
Affiliation:
Center for Basic Psychiatric Research, Aarhus University Hospital, Risskov, Denmark
G. Winther
Affiliation:
Center for Basic Psychiatric Research, Aarhus University Hospital, Risskov, Denmark
J. Elnif
Affiliation:
Animal Nutrition, Department of Basic Animal and Veterinary Sciences, University of Copenhagen, Copenhagen, Denmark
S. Frische
Affiliation:
The Water and Salt Research Centre, Institute of Anatomy, University of Aarhus, Aarhus C, Denmark
T. Wang
Affiliation:
Department of Bioscience, Zoophysiology, University of Aarhus, Aarhus C, Denmark
D. Mayntz
Affiliation:
Department of Bioscience, Ecology and Genetics, University of Aarhus, Aarhus C, Denmark Department of Genetics and Biotechnology, University of Aarhus, Research Centre Foulum, Tjele, Denmark
*
*Address for correspondence: A. Dudele, M.Sc., Department of Bioscience, Zoophysiology, University of Aarhus, C.F. Møllers allé, Building 1131, DK-8000, Aarhus C, Denmark. (Email [email protected])
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Abstract

Dietary protein restriction in pregnant females reduces offspring birth weight and increases the risk of developing obesity, type 2 diabetes and cardiovascular disease. Despite these grave consequences, few studies have addressed the effects of preconceptional maternal malnutrition. Here we investigate how a preconceptional low-protein (LP) diet affects offspring body mass and insulin-regulated glucose metabolism. Ten-week-old female mice (C57BL/6JBom) received either an LP or isocaloric control diet (8% and 22% crude protein, respectively) for 10 weeks before conception, but were thereafter fed standard laboratory chow (22.5% crude protein) during pregnancy, lactation and offspring growth. When the offspring were 10 weeks old, they were subjected to an intraperitoneal glucose tolerance test (GTT), and sacrificed after a 5-day recovery period to determine visceral organ mass. Body mass of LP male offspring was significantly lower at weaning compared with controls. A similar, nonsignificant, tendency was observed for LP female offspring. These differences in body mass disappeared within 1 week after weaning, a consequence of catch-up growth in LP offspring. GTTs of 10-week-old offspring revealed enhanced insulin sensitivity in LP offspring of both sexes. No differences were found in body mass, food intake or absolute size of visceral organs of adult offspring. Our results indicate that maternal protein restriction imposed before pregnancy produces effects similar to postconceptional malnutrition, namely, low birth weight, catch-up growth and enhanced insulin sensitivity at young adulthood. This could imply an increased risk of offspring developing lifestyle-acquired diseases during adulthood.

Keywords

glucose tolerancelow-protein dietpreconception nutrition
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 3 , Issue 5 , October 2012 , pp. 364 - 374
Copyright
Copyright © Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2012

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