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Low levels of sulfur and cobalt during the pre- and periconceptional periods affect the oocyte yield of donors and the DNA methylome of preimplantation bovine embryos

Published online by Cambridge University Press:  04 May 2021

Allice R. Ferreira Nochi ,
Luna N. Vargas ,
Roberto Sartori ,
Roberto G. Júnior ,
Davi B. Araújo ,
Ricardo A. Figueiredo ,
Roberto C. Togawa ,
Marcos M. C. Costa ,
Priscila Grynberg  and
Anelise S. Mendonça
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Allice R. Ferreira Nochi
Affiliation:
Department of Animal Reproduction and Veterinary Radiology, State University of São Paulo “Júlio of Mesquita Filho’’ College of Medicine Veterinary and Animal Science, Botucatu, São Paulo, Brazil Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil
Luna N. Vargas
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil
Roberto Sartori
Affiliation:
Department of Animal Science, University of São Paulo, Piracicaba, São Paulo, Brazil
Roberto G. Júnior
Affiliation:
Embrapa Cerrados, Planaltina, Distrito Federal, Brazil
Davi B. Araújo
Affiliation:
Cargill Animal Nutrition, Campinas, São Paulo, Brazil
Ricardo A. Figueiredo
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil
Roberto C. Togawa
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil
Marcos M. C. Costa
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil
Priscila Grynberg
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil
Anelise S. Mendonça
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil
Nayara R. Kussano
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil
Ivo Pivato
Affiliation:
Animal Reproduction Laboratory, College of Agronomy and Veterinary Medicine, University of Brasilia, Brasília, Distrito Federal, Brazil
Bianca D. M. Silva
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil
José Felipe W. Spricigo
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil
Ligiane O. Leme
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil
Joseane P. da Silva
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil
Alexandre R. Caetano
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil
Margot A. N. Dode
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil
Maurício M. Franco*
Affiliation:
Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil
*
Address for correspondence: Maurício M. Franco, Embrapa Genetic Resources and Biotechnology, Laboratory of Animal Reproduction, Parque Estação Biológica, W5 Norte Final, Brasília70770-917, DF, Brazil. Email: [email protected]
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Abstract

Maternal nutrition is critical in mammalian development, influencing the epigenetic reprogramming of gametes, embryos, and fetal programming. We evaluated the effects of different levels of sulfur (S) and cobalt (Co) in the maternal diet throughout the pre- and periconceptional periods on the biochemical and reproductive parameters of the donors and the DNA methylome of the progeny in Bos indicus cattle. The low-S/Co group differed from the control with respect to homocysteine, folic acid, B12, insulin growth factor 1, and glucose. The oocyte yield was lower in heifers from the low S/Co group than that in the control heifers. Embryos from the low-S/Co group exhibited 2320 differentially methylated regions (DMRs) across the genome compared with the control embryos. We also characterized candidate DMRs linked to the DNMT1 and DNMT3B genes in the blood and sperm cells of the adult progeny. A DMR located in DNMT1 that was identified in embryos remained differentially methylated in the sperm of the progeny from the low-S/Co group. Therefore, we associated changes in specific compounds in the maternal diet with DNA methylation modifications in the progeny. Our results help to elucidate the impact of maternal nutrition on epigenetic reprogramming in livestock, opening new avenues of research to study the effect of disturbed epigenetic patterns in early life on health and fertility in adulthood. Considering that cattle are physiologically similar to humans with respect to gestational length, our study may serve as a model for studies related to the developmental origin of health and disease in humans.

Keywords

Epigeneticsintergenerational inheritancelivestock
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 13 , Issue 2 , April 2022 , pp. 231 - 243
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Copyright
© The Author(s), 2021. Published by Cambridge University Press in association with International Society for Developmental Origins of Health and Disease

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