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The influence of dexamethasone administered prenatally on cartilage of newborn spiny mouse (Acomys cahirinus) offspring

Published online by Cambridge University Press:  17 November 2015

P. Iwaniak ,
P. Dobrowolski ,
E. Tomaszewska ,
M. Hułas-Stasiak ,
A. Tomczyk  and
A. Gawron
P. Iwaniak*
Affiliation:
Department of Comparative Anatomy and Anthropology, Maria Curie-Sklodowska University, Lublin, Poland
P. Dobrowolski
Affiliation:
Department of Comparative Anatomy and Anthropology, Maria Curie-Sklodowska University, Lublin, Poland
E. Tomaszewska
Affiliation:
Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland
M. Hułas-Stasiak
Affiliation:
Department of Comparative Anatomy and Anthropology, Maria Curie-Sklodowska University, Lublin, Poland
A. Tomczyk
Affiliation:
Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland
A. Gawron
Affiliation:
Department of Comparative Anatomy and Anthropology, Maria Curie-Sklodowska University, Lublin, Poland
*
*Address for correspondence: P. Iwaniak, Department of Comparative Anatomy and Anthropology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland. (Email [email protected])
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Abstract

Considering the negative effects of glucocorticoid treatment, especially during fetal development it is important to investigate effectors decreasing such disadvantages. The aim of this study was to investigate the effect of prenatally administered dexamethasone (Dex), a synthetic glucocorticoid, on the histomorphometry of the femur in the offspring of spiny mice. The study was performed on 24 pregnant spiny mice. The time of the experiment included the prenatal period between the 20th day of gestation until birth (pregnancy lasts on average of 36–38 days). The mice from the experimental group received dexamethasone per os in a dose of 125 mg/kg birth weight daily. At the end, the newborns from the experimental and control group were weighted and euthanized. Maternal Dex treatment resulted in a 17% decrease in birth weight in newborns. Dex administration significantly reduced the thickness of the hypertrophy zone of the growth plate by 34% and total thickness by 8,7%. In addition, Dex decreased the number of cells in the articular cartilage by 27% and significantly decreased their diameter by 5%. Dex also affected the structure and spatial distribution of thick and thin collagen fibers, lowering the proportion of thin fibers compared with the control group. Moreover, Dex treatment considerably lowered the amount of proteoglycans in articular and growth cartilages. Exposure to glucocorticoids in pregnant spiny mice affects cartilage development by accelerating maturity of collagen fibers and growth plate, presumably along with further disruption of longitudinal growth of long bones.

Keywords

Acomys cahirinuscartilagedexamethasoneprenatal treatment
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 7 , Issue 3: Themed Issue: Ibero-American DOHaD Society , June 2016 , pp. 298 - 305
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Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2015 

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