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Feeding a protein-restricted diet during pregnancy induces altered epigenetic regulation of peroxisomal proliferator-activated receptor-α in the heart of the offspring

Published online by Cambridge University Press:  05 August 2010

J. L. Slater-Jefferies ,
K. A. Lillycrop ,
P. A. Townsend ,
C. Torrens ,
S. P. Hoile ,
M. A. Hanson  and
G. C. Burdge
J. L. Slater-Jefferies
Affiliation:
Institute of Developmental Sciences, School of Medicine, University of Southampton, Southampton, UK
K. A. Lillycrop
Affiliation:
Development and Cell Biology, School of Biological Sciences, University of Southampton, UK
P. A. Townsend
Affiliation:
Human Genetics Division, School of Medicine, University of Southampton, Southampton, UK
C. Torrens
Affiliation:
Institute of Developmental Sciences, School of Medicine, University of Southampton, Southampton, UK
S. P. Hoile
Affiliation:
Institute of Developmental Sciences, School of Medicine, University of Southampton, Southampton, UK
M. A. Hanson
Affiliation:
Institute of Developmental Sciences, School of Medicine, University of Southampton, Southampton, UK
G. C. Burdge*
Affiliation:
Institute of Developmental Sciences, School of Medicine, University of Southampton, Southampton, UK
*
*Address for correspondence: G. C. Burdge, Institute of Developmental Sciences, School of Medicine, University of Southampton, Southampton, SO16 6YD, UK. (Email [email protected])
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Abstract

Impaired flexibility in the use of substrates for energy production in the heart is implicated in cardiomyopathy. We investigated the effect of maternal protein restriction during pregnancy in rats on the transcription of key genes in cardiac lipid and carbohydrate metabolism in the offspring. Rats were fed protein-sufficient or protein-restricted (PR) diets during pregnancy. Triacylglycerol concentration in adult (day 105) heart was altered by maternal protein intake contingent on post-weaning fat intake and sex. mRNA expression of peroxisomal proliferator-activated receptor (PPAR)-α and carnitine palmitoyltransferase-1 was increased by the maternal PR diet in adult, but not neonatal, offspring. PPARα promoter methylation was lower in adult and neonatal heart from PR offspring. These findings suggest that prenatal nutrition alters the future transcriptional regulation of cardiac energy metabolism in the offspring through changes in epigenetic regulation of specific genes. However, changes in gene functional changes may not be apparent in early life.

Keywords

cardiomyopathyepigeneticheartPPARprotein restriction
Type
Brief Report
Information
Journal of Developmental Origins of Health and Disease , Volume 2 , Issue 4 , August 2011 , pp. 250 - 255
Copyright
Copyright © Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2010

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