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Characterization of the role of microRNA-517a expression in low birth weight infants

Published online by Cambridge University Press:  26 June 2013

G. Y. Song ,
W. W. Song ,
Y. Han ,
D. Wang  and
Q. Na
G. Y. Song
Affiliation:
Department of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning Province, China
W. W. Song*
Affiliation:
Department of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning Province, China
Y. Han
Affiliation:
Department of Gynecology and Obstetrics, Shenyang Women and Children's Hospital, Shenyang, Liaoning Province, China
D. Wang
Affiliation:
Department of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning Province, China
Q. Na
Affiliation:
Department of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning Province, China
*
*Address for correspondence: W. W. Song, Department of Gynaecology and Obstetrics, Shengjing Hospital of China Medical University, No. 36, San Hao Street, Shenyang, Liaoning 110004, P.R. China. (Email [email protected])
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Abstract

The purpose of this study was to analyze the expression of the placenta-specific microRNA miR-517a in maternal serum and in placental tissue from low birth weight newborns and try to detect the effects of miR-517a expression on invasion potential of trophoblasts. Placental tissue and maternal serum were collected from both low birth weight newborns (n = 10) and normal birth weight newborns (n = 20). Expression of miR-517a was assessed in placenta and serum samples by real-time qRT-PCR. In addition, human trophoblast HTR8/SVneo cells were transfected with a miR-517a 2′-O-methyl oligonucleotide or a negative control RNA, and invasion was measured using transwell migration assays. Expression of miR-517a was significantly increased in placentas from low birth weight newborns (61.79 ± 23.06) in comparison with those of normal birth weight newborns (5.01 ± 1.97; P < 0.05). The expression of miR-517a was also increased in maternal serum isolated from the low birth weight newborn (25.78 ± 8.69) compared with the normal birth weight newborn (3.21 ± 1.07; P < 0.05). Overexpression of miR-517a significantly inhibited invasion of HTR8/SVneo cells (P < 0.05). These data indicate that miR-517a overexpression could potentially lead to low birth weight, likely through the inhibition of trophoblast invasion.

Keywords

birth weightmiR-517aplacentatrophoblast invasion
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 4 , Issue 6 , December 2013 , pp. 522 - 526
Copyright
Copyright © Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2013 

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