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Breastfeeding: a key modulator of gut microbiota characteristics in late infancy

Published online by Cambridge University Press:  19 November 2018

M. Matsuyama Open the ORCID record for M. Matsuyama [Opens in a new window] ,
L. F. Gomez-Arango ,
N. M. Fukuma ,
M. Morrison ,
P. S. W. Davies  and
R. J. Hill
M. Matsuyama*
Affiliation:
Children’s Nutrition Research Centre, UQ Child Health Research Centre, Faculty of Medicine, The University of Queensland, South Brisbane, QLD, Australia
L. F. Gomez-Arango
Affiliation:
School of Chemistry and Molecular Biosciences, Faculty of Science, The University of Queensland, St Lucia, QLD, Australia
N. M. Fukuma
Affiliation:
Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
M. Morrison
Affiliation:
Faculty of Medicine, Diamantina Institute, The University of Queensland, Brisbane, QLD, Australia Translational Research Institute, Woolloongabba, QLD, Australia
P. S. W. Davies
Affiliation:
Children’s Nutrition Research Centre, UQ Child Health Research Centre, Faculty of Medicine, The University of Queensland, South Brisbane, QLD, Australia
R. J. Hill
Affiliation:
Children’s Nutrition Research Centre, UQ Child Health Research Centre, Faculty of Medicine, The University of Queensland, South Brisbane, QLD, Australia
*
Address for correspondence: M. Matsuyama, Children’s Nutrition Research Centre, UQ Child Health Research Centre, Faculty of Medicine, The University of Queensland, 62 Graham Street, South Brisbane, QLD 4101, Australia. E-mail: [email protected]
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Abstract

The objective of this study was to investigate the impact of the most commonly cited factors that may have influenced infants’ gut microbiota profiles at one year of age: mode of delivery, breastfeeding duration and antibiotic exposure. Barcoded V3/V4 amplicons of bacterial 16S-rRNA gene were prepared from the stool samples of 52 healthy 1-year-old Australian children and sequenced using the Illumina MiSeq platform. Following the quality checks, the data were processed using the Quantitative Insights Into Microbial Ecology pipeline and analysed using the Calypso package for microbiome data analysis. The stool microbiota profiles of children still breastfed were significantly different from that of children weaned earlier (P<0.05), independent of the age of solid food introduction. Among children still breastfed, Veillonella spp. abundance was higher. Children no longer breastfed possessed a more ‘mature’ microbiota, with notable increases of Firmicutes. The microbiota profiles of the children could not be differentiated by delivery mode or antibiotic exposure. Further analysis based on children’s feeding patterns found children who were breastfed alongside solid food had significantly different microbiota profiles compared to that of children who were receiving both breastmilk and formula milk alongside solid food. This study provided evidence that breastfeeding continues to influence gut microbial community even at late infancy when these children are also consuming table foods. At this age, any impacts from mode of delivery or antibiotic exposure did not appear to be discernible imprints on the microbial community profiles of these healthy children.

Keywords

first thousand daysformula feedinggut microbiomegut microbiotalate infancymixed feeding
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 10 , Issue 2 , April 2019 , pp. 206 - 213
Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2018. 

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