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Association between extrauterine growth restriction and changes of intestinal flora in Chinese preterm infants

Published online by Cambridge University Press:  05 March 2019

J. Zhang Open the ORCID record for J. Zhang [Opens in a new window] ,
Y. Dai ,
S. Fan ,
K. Zhang ,
C. Shuai ,
X. Bian ,
L. Hui ,
Z. Wu ,
J. Zhang  and
Z. Guo
...Show all authors
J. Zhang*
Affiliation:
Department of Pediatric, East Campus of Shanghai Sixth People’s Hospital, Shanghai University of Medicine & Health Sciences, Shanghai Jiao Tong University, Shanghai, China
Y. Dai
Affiliation:
Department of Neonatology, Children’s Hospital of Fudan University, Shanghai, China
S. Fan
Affiliation:
Department of Pediatric, East Campus of Shanghai Sixth People’s Hospital, Shanghai University of Medicine & Health Sciences, Shanghai Jiao Tong University, Shanghai, China
K. Zhang
Affiliation:
Department of Pediatric, East Campus of Shanghai Sixth People’s Hospital, Shanghai University of Medicine & Health Sciences, Shanghai Jiao Tong University, Shanghai, China
C. Shuai
Affiliation:
Maternal and Child Health Hospital of Guangdong Province, Guangzhou, China
X. Bian
Affiliation:
Department of Pediatric, East Campus of Shanghai Sixth People’s Hospital, Shanghai University of Medicine & Health Sciences, Shanghai Jiao Tong University, Shanghai, China
L. Hui
Affiliation:
Department of Pediatric, East Campus of Shanghai Sixth People’s Hospital, Shanghai University of Medicine & Health Sciences, Shanghai Jiao Tong University, Shanghai, China
Z. Wu
Affiliation:
Department of Pediatric, East Campus of Shanghai Sixth People’s Hospital, Shanghai University of Medicine & Health Sciences, Shanghai Jiao Tong University, Shanghai, China
J. Zhang
Affiliation:
Department of Pediatric, East Campus of Shanghai Sixth People’s Hospital, Shanghai University of Medicine & Health Sciences, Shanghai Jiao Tong University, Shanghai, China
Z. Guo
Affiliation:
Department of Pediatric, East Campus of Shanghai Sixth People’s Hospital, Shanghai University of Medicine & Health Sciences, Shanghai Jiao Tong University, Shanghai, China
F. Deng
Affiliation:
Department of Pediatric, East Campus of Shanghai Sixth People’s Hospital, Shanghai University of Medicine & Health Sciences, Shanghai Jiao Tong University, Shanghai, China
M. Guo
Affiliation:
Department of Pediatric, East Campus of Shanghai Sixth People’s Hospital, Shanghai University of Medicine & Health Sciences, Shanghai Jiao Tong University, Shanghai, China
*
Address for correspondence: J. Zhang, Department of Pediatrics, East Campus of Shanghai Sixth People’s Hospital, Affiliated to Shanghai University of Medicine & Health Sciences Affiliated to Shanghai Jiaotong University, No. 222 Huanhuxisan Road, Pudongxin District, Shanghai 201306, China. E-mail: [email protected]
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Abstract

The aim of the study was to investigate any association between extrauterine growth restriction (EUGR) and intestinal flora of <30-week-old preterm infants. A total of 59 preterm infants were assigned to EUGR (n=23) and non-EUGR (n=36) groups. Intestinal bacteria were compared by using high-throughput sequencing of bacterial rRNA. The total abundance of bacteria in 344 genera (7568 v. 13,760; P<0.0001) and 456 species (10,032 v. 18,240; P<0.0001) was significantly decreased in the EUGR group compared with the non-EUGR group. After application of a multivariate logistic model and adjusting for potential confounding factors, as well as false-discovery rate corrections, we found four bacterial genera with higher and one bacterial genus with lower abundance in the EUGR group compared with the control group. In addition, the EUGR group showed significantly increased abundances of six species (Streptococcus parasanguinis, Bacterium RB5FF6, two Klebsiella species and Microbacterium), but decreased frequencies of three species (one Acinetobacter species, Endosymbiont_of_Sphenophorus_lev and one Enterobacter_species) compared with the non-EUGR group. Taken together, there were significant changes in the intestinal microflora of preterm infants with EUGR compared to preterm infants without EUGR.

Keywords

classification of bacteriaextrauterine growth restriction (EUGR)gastrointestinal bacteriahigh-throughput sequencingpreterm infant
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 10 , Issue 5 , October 2019 , pp. 513 - 521
Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2019 

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Footnotes

†

Jinping Zhang, Yi Dai and Sainan Fan contributed equally to the article.

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