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Variations in the levels of acute-phase proteins and lactoferrin in serum and milk during bovine subclinical mastitis

Published online by Cambridge University Press:  12 August 2021

Aarsha Raj
Affiliation:
Multi-Speciality Veterinary Hospital, Thiruvanathapuram, Kerala, India
Vinodkumar Kulangara*
Affiliation:
Department of Veterinary Epidemiology and Preventive Medicine, Kerala, India
Tresamol P. Vareed
Affiliation:
Department of Veterinary Epidemiology and Preventive Medicine, Kerala, India
Deepa P. Melepat
Affiliation:
Department of Veterinary Epidemiology and Preventive Medicine, Kerala, India
Latha Chattothayil
Affiliation:
Department of Veterinary Public Health, Kerala, India
Sunanda Chullipparambil
Affiliation:
Department of Statistics, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, India
*
Author for correspondence: Vinodkumar Kulangara, Email: [email protected]

Abstract

Variations in the levels of acute phase proteins and lactoferrin in serum and milk for diagnosis of subclinical mastitis in dairy cows are described in this research paper. Milking animals from two organized dairy farms in Kerala, India, were screened by California Mastitis Test (CMT), Electrical Conductivity test (EC) and Somatic Cell Count (SCC) test to identify animals affected with sub clinical mastitis (SCM). The concentrations of acute phase proteins (APP) Haptoglobin (Hp), C- reactive protein (CRP), Albumin, Lactoferrin (Lf) and α- 1 acid glycoprotein (AGP) in milk and Hp, Albumin, Serum Amyloid A (SAA) and CRP in the serum of 40 normal cows and 40 cows affected with sub clinical mastitis were assessed. Solid phase ELISA was employed for assessment of all parameters except the albumin levels, for which spectrophotometry was used. The values of Hp in milk; and SAA, AGP and Lf in serum, were significantly elevated in the group with sub clinical mastitis. Such variations were found to be independent of the specific bacterial organism causing the disease. These results show that significant variations exist in the levels of acute phase proteins Hp, AGP and Lf in milk, and SAA in serum of animals affected with subclinical bovine mastitis that are not affected by specific bacterial etiology.

Type
Research Article
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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References

Akerstedt, M, Waller, KP and Sternesjo, A (2007) Haptoglobin and serum amyloid A in relation to the somatic cell count in quarter, cow composite and bulk tank milk samples. Journal of Dairy Research 74, 198203.10.1017/S0022029906002305CrossRefGoogle ScholarPubMed
Batavani, RA, Asri, S and Naebzadeh, H (2007) The effect of subclinical mastitis on milk composition in dairy cows. Iranian Journal of Veterinary Research 8, 205211.Google Scholar
Biggadike, HJ, Ohnstad, I, Laven, RA and Hillerton, JE (2002) Evaluation of measurements of the conductivity of quarter milk samples for the early diagnosis of mastitis. Veterinary Record 150, 655658.10.1136/vr.150.21.655CrossRefGoogle ScholarPubMed
Ceciliani, F, Pocacqua, V, Lecchi, C, Fortin, R, Rebucci, R, Avallone, G, Bronzo, V, Cheli, F and Sartorelli, P (2007) Differential expression and secretion of α 1-acid glycoprotein in bovine milk. Journal of Dairy Research 74, 374380.10.1017/S0022029907002646CrossRefGoogle Scholar
Dalanezi, FM, Schmidt, EMS, Joaquim, SF, Guimaraes, FF, Guerra, TS, Lopes, CB, Cerri, RLA, Chadwick, CC and Langoni, H (2020) Concentrations of acute-phase proteins in milk from cows with clinical mastitis caused by different pathogens. Pathogens 9, E706.10.3390/pathogens9090706CrossRefGoogle ScholarPubMed
Doumas, BT, Watson, WA and Biggs, HG (1971) Albumin standards and the measurement of serum albumin with bromcresol green. Clinicachimicaacta 31, 8796.Google ScholarPubMed
Eckersall, PD, Young, FJ, McComb, C, Hogarth, CJ, Safi, S, Weber, A, McDonald, T, Nolan, AM and Fitzpatrick, JL (2001) Acute phase proteins in serum and milk from dairy cows with clinical mastitis. Veterinary Record 148, 3541.10.1136/vr.148.2.35CrossRefGoogle ScholarPubMed
Eckersall, PD, Young, FJ, Nolan, AM, Knight, CH, McComb, C, Waterston, M, Hogarth, CJ, Scott, EM and Fitzpatrick, JL (2006) Acute phase proteins in bovine milk in an experimental model of Staphylococcus aureus subclinical mastitis. Journal of Dairy Science 89, 14881501.10.3168/jds.S0022-0302(06)72216-0CrossRefGoogle Scholar
Fathi, E and Farahzadi, R (2011) Survey on relationship between acute phase proteins (serum amyloid A, milk amyloid A and serum haptoglobin) in inflammatory diseases of dairy cattle. Iranian Journal of Veterinary Science and Technology 3, 5765.Google Scholar
Fetherston, CM, Wells, JI and Hartmann, PE (2006) Severity of mastitis symptoms as a predictor of C-reactive protein in milk and blood during lactation. Breastfeeding Medicine 1, 127135.10.1089/bfm.2006.1.127CrossRefGoogle ScholarPubMed
Galfi, AL, Radinović, , Boboš, SF, Pajić, MJ, Savić, SS and Milanov, DS (2016) Lactoferrin concentrations in bovine milk during involution of the mammary glands with different bacteriological findings. Veterinaryarhiv 86, 487497.Google Scholar
Gerardo, G, Bernardini, D, Elia, CA, Ferrari, V, Iob, L and Segato, S (2009) Use of serum amyloid A and milk amyloid A in the diagnosis of subclinical mastitis in dairy cows. Journal of Dairy Research 76, 411.10.1017/S0022029909990057CrossRefGoogle Scholar
Guha, A, Guha, R and Gera, S (2013) Comparison of α1-antitrypsin, α 1–acid glycoprotein, fibrinogen and NOx as indicator of subclinical mastitis in riverine buffalo (Bubalus bubalis) Asian australas. Journal of Animal Science 26, 788794.Google Scholar
Hegde, R, Isloor, S, Prabhu, KN, Shome, BR, Rathnamma, D, Suryanarayana, VV, Yatiraj, S, Prasad, CR, Krishnaveni, N, Sundareshan, S, Akhila, DS, Gomes, AR and Hegde, NR (2013) Incidence of subclinical mastitis and prevalence of major mastitis pathogens in organized farms and unorganized sectors. Indian Journal of Microbiology 53, 315320.10.1007/s12088-012-0336-1CrossRefGoogle ScholarPubMed
Huang, YQ, Morimoto, K, Hosoda, K, Yoshimura, Y and Isobe, N (2012) Differential immunolocalization between lingual antimicrobial peptide and lactoferrin in mammary gland of dairy cows. Veterinary Immunology and Immunopathology 145, 499504.10.1016/j.vetimm.2011.10.017CrossRefGoogle ScholarPubMed
Kovac, G, Popelkova, M, Tkaaikova, B and Ihnat, O (2007) Interrelationship between somatic cell count and acute phase proteins in serum and milk of dairy cows. Acta Veterinary Brno 76, 5157.10.2754/avb200776010051CrossRefGoogle Scholar
Mold, C, Rodic-Polic, B and Du Clos, TW (2002) Protection from Streptococcus pneumoniae infection by C-reactive protein and natural antibody requires complement but not Fc-gamma receptors. Journal of Immunology 168, 63756381.10.4049/jimmunol.168.12.6375CrossRefGoogle Scholar
Murata, H, Shimada, N and Yoshioka, M (2004) Current research on acute phase proteins in veterinary diagnosis: an overview. Veterinary Journal 168, 2840.10.1016/S1090-0233(03)00119-9CrossRefGoogle ScholarPubMed
O'Mahony, MC, Healy, AM, Harte, D, Walshe, KG, Torgerson, PR and Doherty, ML (2006) Milk amyloid A:correlation with cellular indices of mammary inflammation in cows with normal and raised serum amyloid A. Research in Veterinary Science 80, 155161.10.1016/j.rvsc.2005.05.005CrossRefGoogle ScholarPubMed
Panicker, VP, Gopalakrishnan, A and George, S (2014) Acute phase proteins of veterinary importance. World Journal of Pharmacy and Pharmaceutical Science 3, 188195.Google Scholar
Pyörälä, S and Syväjärvi, J (1987) Bovine acute mastitis part I. Clinical aspects and parameters of inflammation in mastitis caused by different pathogens. Journal of Veterinary Medicine, Series B 34, 573584.10.1111/j.1439-0450.1987.tb00438.xCrossRefGoogle Scholar
Pyorala, S, Hovinen, M, Simojoki, H, Fitzpatrick, J, Eckersall, PD and Orro, T (2011) Acute phase proteins in milk in naturally acquired bovine mastitis caused by different pathogens. Veterinary Record 168, 535542.10.1136/vr.d1120CrossRefGoogle ScholarPubMed
Razak, R, Hussain, I, Dar, PA and Mir, SB (2015) Relationship between serum amyloid A3 in serum and milk of mastitic cows. Applied Biological Research 17, 315319.10.5958/0974-4517.2015.00046.4CrossRefGoogle Scholar
Sadek, K, Saleh, E and Ayoub, M (2016) Selective, reliable blood and milk biomarkers for diagnosing clinical and subclinical bovine mastitis. Tropical Animal Health and Production 49, 431437.10.1007/s11250-016-1190-7CrossRefGoogle ScholarPubMed
Safi, S, Khoshvaghti, A, Jafarzadeh, SR, Bolourchi, M and Nowrouzian, I (2009) Acute phase proteins in the diagnosis of bovine subclinical mastitis. Veterinary Clinical Pathology 38, 471476.10.1111/j.1939-165X.2009.00156.xCrossRefGoogle Scholar
Schukken, YH, Wilson, DJ, Welcome, F, Garrison-Tinofsky, L and Gonzales, RN (2003) Monitoring udder health and milk quality using somatic cell counts. Veterinary Research 34, 579596.10.1051/vetres:2003028CrossRefGoogle ScholarPubMed
Sharma, N, Pandey, V and Sudhan, NA (2010) Comparison of some indirect screening tests for detection of subclinical mastitis in dairy cows. Bulgarian Journal of Veterinary Research 13, 98103.Google Scholar
Shimazaki, K-I and Kawai, K (2017) Advances in lactoferrin research concerning bovine mastitis. Biochemistry and Cell Biology 95, 6975.10.1139/bcb-2016-0044CrossRefGoogle ScholarPubMed
Simões, PBA, Campbell, M, Viora, L, Gibbons, J, Geraghty, TE, Eckersall, PD and Zadoks, R (2017) Pilot study into milk haptoglobin as an indicator of udder health in heifers after calving. Research in Veterinary Science 116, 8387.10.1016/j.rvsc.2017.05.024CrossRefGoogle ScholarPubMed
Singh, R, Bhardwaj, RK, Azad, MS and Beigh, SA (2014) Effect of mastitis on haemato-biochemical and plasma mineral profile in crossbred cattle. Indian Journal of Animal Research 48, 6366.10.5958/j.0976-0555.48.1.013CrossRefGoogle Scholar
Thomas, FC (2015) Acute phase proteins, proteomics and metabolomics in the diagnosis of bovine mastitis. PhD thesis, University of Glasgow, 395p.Google Scholar
Thomas, FC, Waterston, M, Hastie, P, Parking, T, Haining, H and Eckersall, PD (2015) The major acute phase proteins of bovine milk in a commercial dairy herd. BMC Veterinary Research 11, 207217.10.1186/s12917-015-0533-3CrossRefGoogle Scholar
Thomas, FC, Geraghty, T, Simões, PBA, Mshelbwala, FM, Haining, H and Eckersall, PD (2018) A pilot study of acute phase proteins as indicators of bovine mastitis caused by different pathogens. Research in Veterinary Science 119, 76181.10.1016/j.rvsc.2018.06.015CrossRefGoogle ScholarPubMed
Tothova, C, Nagy, O and Kovac, G (2014) Acute phase proteins and their use in diagnosis of diseases in ruminants. Veterinarni Medicina 74, 163180.10.17221/7478-VETMEDCrossRefGoogle Scholar
Upadhyaya, I, Thanislass, J, Veerapandyan, A, Badami, S and Antony, PX (2016) Characterization of haptoglobin isotype in milk of mastitis-affected cows. Veterinary Sciences 3, 2934.10.3390/vetsci3040029CrossRefGoogle ScholarPubMed
Vasiu, I, Dabrowski, R, Martinez-Subiela, S, Ceron, JJ, Wdowiak, A, Pop, RA, Brudasca, FG, Pastor, J and Tvarijonaviciute, A (2017) Milk C-reactive protein in canine mastitis. Veterinary Immunology and Immunopathology 186, 4144.10.1016/j.vetimm.2017.02.005CrossRefGoogle ScholarPubMed
Wellenberg, GJ, Van der Poel, WH and Van Oirshot, JT (2002) Viral infections and bovine mastitis: a review. Veterinary Microbiology 88, 2745.10.1016/S0378-1135(02)00098-6CrossRefGoogle ScholarPubMed
Zalewska, M, Kawecka-Grochocka, E, Słoniewska, D, Marczak, S, Jarmuz, W, Zweirzchowski, L and Bagnicka, E (2020) Acute phase protein expressions in secretory and cistern lining epithelium tissues of the dairy cattle mammary gland during chronic mastitis caused by staphylococci. BMC Veterinary Research 16, 320.10.1186/s12917-020-02544-8CrossRefGoogle ScholarPubMed
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