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Variability of the caprine whey protein genes and their association with milk yield, composition and renneting properties in the Sarda breed. 1. The LALBA gene

Published online by Cambridge University Press:  25 August 2015

Maria Luisa Dettori ,
Michele Pazzola ,
Pietro Paschino ,
Maria Giovanna Pira  and
Giuseppe Massimo Vacca
Maria Luisa Dettori*
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna, 2 07100 Sassari, Italy
Michele Pazzola
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna, 2 07100 Sassari, Italy
Pietro Paschino
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna, 2 07100 Sassari, Italy
Maria Giovanna Pira
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna, 2 07100 Sassari, Italy
Giuseppe Massimo Vacca
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna, 2 07100 Sassari, Italy Centro di Competenza Biodiversità Animale, viale Adua 2C, 07100 Sassari, Italy
*
*For correspondence; e-mail: [email protected]
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Abstract

The 5′ flanking region and 3′ UTR of the caprine LALBA gene were analysed by SSCP and sequencing. A total of nine SNPs were detected: three in the promoter region, two were synonymous coding SNPs at exon-1, and four SNPs were in exon-4, within the 3′UTR. The nucleotide changes located in the promoter region (c.−358T>C, c.−163G>A, c.−121T>G) were genotyped by SSCP in 263 Sarda goats to evaluate their possible effect on milk yield, composition and renneting properties. We observed an effect of the three SNPs on milk yield and lactose content. Genotypes TT and CT at c.−358T>C (P < 0·001) and genotypes AG and GG at c.−163G>A (P < 0·01) were characterised by higher lactose contents, while c.−358CC and c.−163AA showed the lower milk yield (P < 0·01). SNPs c.−358T>C and c.−121T>G were part of transcription factors binding sites, potentially involved in modulating the LALBA gene expression. The LALBA genotype affected renneting properties (P < 0·001), as heterozygotes c.−358CT and c.−163GA were characterised by delayed rennet coagulation time and curd firming time and the lowest value of curd firmness. The present investigation increases the panel of SNPs and adds new information about the effects of the caprine LALBA gene polymorphism.

Keywords

Goat milkalpha-lactalbuminLALBArenneting properties
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 4 , November 2015 , pp. 434 - 441
Copyright
Copyright © Proprietors of Journal of Dairy Research 2015 

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