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Use of lyophilized skin for testing the bactericidal activity of teat disinfectants

Published online by Cambridge University Press:  01 June 2009

A. John Bramley
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9 AT, UK
Elizabeth M. Hogben
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9 AT, UK

Summary

The bactericidal activities of various concentrations of 3 disinfectants commonly used for teat disinfection were compared in vitro using small discs of pig skin previously contaminated with Staphylococcus aureus. Solutions containing 40 g/1 Na hypochlorite, 25 or 50 g/1 chlorhexidine digluconate or iodophor containing 5 g/1 iodine were found to have equivalent or superior bactericidal activity to a solution containing 10 g/1 Na hypochlorite. This in vitro technique offers a convenient alternative to in vivo tests for teat disinfectants and with minor modifications could be applicable to the testing of skin antiseptics in general.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1983

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References

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