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Ultrasonication and the quality of human milk: variation of power and time of exposure

Published online by Cambridge University Press:  05 July 2012

Lukas Christen ,
Ching Tat Lai  and
Peter E. Hartmann
Lukas Christen*
Affiliation:
School of Chemistry and Biochemistry, Faculty of Life and Physical Sciences, M310, The University of Western Australia, Crawley, WA 6009, Australia Carag AG, Bahnhofstrasse 9, CH-6340 Baar, Switzerland
Ching Tat Lai
Affiliation:
School of Chemistry and Biochemistry, Faculty of Life and Physical Sciences, M310, The University of Western Australia, Crawley, WA 6009, Australia
Peter E. Hartmann
Affiliation:
School of Chemistry and Biochemistry, Faculty of Life and Physical Sciences, M310, The University of Western Australia, Crawley, WA 6009, Australia
*
*For correspondence; e-mail: [email protected]
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Abstract

Donor human milk is pasteurized to prevent the potential risk of the transmission of pathogens to preterm infants. Currently, Holder pasteurization (human milk held at 62·5°C for 30 min) is used in most human milk banks, but has the disadvantage that it results in excessive inactivation of important bioactive components. Power-ultrasound (20–100 kHz) is an emerging technology for the preservation of foods and could be an alternative method for the treatment of human milk. The aim of this study was to investigate the effect of different ultrasound settings on the elimination of Escherichia coli and the retention of bile salt stimulated lipase (BSSL) activity. Ultrasonication with a constant power decreased Esch. coli viability exponentially over time until the processing temperature increased to sub-pasteurization level to between 51·4 and 58·5°C, then a log10 1·3 decrease was observed (P<0·05). BSSL activity decreased to 91% until a temperature of 51·4°C and then it decreased to 8% between 51·4 and 64·9°C. Ultrasonication with a constant energy and various power and exposure times showed the highest temperature (53·7°C) when treated with the longest exposure time and lowest ultrasound-power (276 s at 3·62 W) compared with 37·6°C for 39 s at 25·64 W. The findings predict that the viability of Esch. coli could be reduced by log10 5 with a minimal loss of activity of BSSL by applying 13·8 kJ of energy in 12 ml of human milk using high ultrasound power over a short exposure time to ensure that the temperature remains below the critical level for protein denaturation. Alternatively, the use of lower power settings such as the 26 W used in the present studies would require a cooling system to ensure the human milk BSSL was protected against temperature denaturation.

Keywords

Donor human milkholder pasteurizationultrasonicationEsch. colibile salt stimulated lipase
Type
Research Article
Information
Journal of Dairy Research , Volume 79 , Issue 3 , August 2012 , pp. 361 - 366
Copyright
Copyright © Proprietors of Journal of Dairy Research 2012

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References

Allison, DG, D'Emanuele, A, Eginton, P & Williams, AR 1996 The effect of ultrasound on Escherichia coli viability. Journal of Basic Microbiology 36 3–11 CrossRefGoogle ScholarPubMed
Andersson, Y, Sävman, K, Bläckberg, L & Hernell, O 2007 Pasteurization of mother's own milk reduces fat absorption and growth in preterm infants. Acta Paediatrica 96 1445–1449 CrossRefGoogle ScholarPubMed
Arnold, L & Tully, M 1994 Guidelines for the Establishment and Operation of a Donor Human Milk Bank. HMBANA West Hartford, CT, USAGoogle Scholar
Arroyo, C, Cebrián, G, Pagán, R & Condón, S 2011 Inactivation of Cronobacter sakazakii by ultrasonic waves under pressure in buffer and foods. International Journal of Food Microbiology 144 446–454 CrossRefGoogle ScholarPubMed
Balmer, SE & Williams, AF 1995 Guidelines for the establishment and operation of human milk banks in the UK. Archives of Disease in Childhood 73 481–482 CrossRefGoogle ScholarPubMed
Cameron, M, McMaster, LD & Britz, TJ 2008 Electron microscopic analysis of dairy microbes inactivated by ultrasound. Ultrasonics Sonochemistry 15 960–964 CrossRefGoogle ScholarPubMed
Chouliara, E, Georgogianni, K, Kanellopoulou, N & Kontominas, M 2010 Effect of ultrasonication on microbiological, chemical and sensory properties of raw, thermized and pasteurized milk. International Dairy Journal 20 307–313 CrossRefGoogle Scholar
Czank, C, Prime, DK, Hartmann, B, Simmer, K & Hartmann, PE 2009 Retention of the immunological proteins of pasteurized human milk in relation to pasteurizer design and practice. Pediatric Research 66 374–379 CrossRefGoogle ScholarPubMed
Czank, C, Simmer, K & Hartmann, PE 2010 Simultaneous pasteurization and homogenization of human milk by combining heat and ultrasound: effect on milk quality. Journal of Dairy Research 77 183–189 CrossRefGoogle ScholarPubMed
D'Amico, DJ, Silk, TM, Wu, J & Guo, M 2006 Inactivation of microorganisms in milk and apple cider treated with ultrasound. Journal of Food Protection 69 556–563 CrossRefGoogle ScholarPubMed
Hartmann, B, Pang, W, Keil, A, Hartmann, PE & Simmer, K 2007 Best practice guidelines for the operation of a donor human milk bank in an Australian NICU. Early Human Development 83 667–673 CrossRefGoogle Scholar
Henderson, TR, Fay, TN & Hamosh, M 1998 Effect of pasteurization on long chain polyunsaturated fatty acid levels and enzyme activities of human milk. Journal of Pediatrics 132 876–878 CrossRefGoogle ScholarPubMed
Hernell, O & Bläckberg, L 1994 Human milk bile salt-stimulated lipase: functional and molecular aspects. Journal of Pediatrics 125 56–61 CrossRefGoogle ScholarPubMed
Monod, J 1949 The growth of bacterial cultures. Annual Review of Microbiology 3 371–94CrossRefGoogle Scholar
Suslick, KS 1990 Sonochemistry. Science 247 1439–1445 CrossRefGoogle ScholarPubMed
Tully, DB, Jones, F & Tully, MR 2001 Donor milk: what's in it and what's not. Journal of Human Lactation 17 152–155 CrossRefGoogle ScholarPubMed
Walkling-Ribeiro, M, Noci, F, Cronin, D, Lyng, J & Morgan, D 2009a Shelf life and sensory evaluation of orange juice after exposure to thermosonication and pulsed electric fields. Food and Bioproducts Processing 87 102–107 CrossRefGoogle Scholar
Walkling-Ribeiro, M, Noci, F, Riener, J, Cronin, D, Lyng, J & Morgan, D 2009b The impact of thermosonication and pulsed electric fields on staphylococcus aureus inactivation and selected quality parameters in orange juice. Food and Bioprocess Technology 2 422–430 CrossRefGoogle Scholar
Wang, J, Hu, X & Wang, Z 2010 Kinetics models for the inactivation of Alicyclobacillus acidiphilus DSM14558(T) and Alicyclobacillus acidoterrestris DSM 3922(T) in apple juice by ultrasound. International Journal of Food Microbiology 139 177–181 CrossRefGoogle ScholarPubMed
Wardell, JM, Wright, AJ, Bardsley, WG & D'Souza, SW 1984 Bile salt-stimulated lipase and esterase activity in human milk after collection, storage, and heating: nutritional implications. Pediatric Research 18 382–386 CrossRefGoogle ScholarPubMed
Wight, NE 2001 Donor human milk for preterm infants. Journal of Perinatology 21 249–254 CrossRefGoogle ScholarPubMed
Williamson, S, Finucane, E & Ellis, H 1978 Effect of heat treatment of human milk on absorption on nitrogen, fat, sodium, calcium and phosphorus by preterm infants. Archives of Disease in Childhood 53 555–563 CrossRefGoogle ScholarPubMed
Wills, ME, Han, VE, Harris, DA & Baum, JD 1982 Short-time low-temperature pasteurisation of human milk. Early Human Development 7 71–80 CrossRefGoogle ScholarPubMed
Zenker, M, Heinz, V & Knorr, D 2003 Application of ultrasound-assisted thermal processing for preservation and quality retention of liquid foods. Journal of Food Protection 66 1642–1649 CrossRefGoogle ScholarPubMed