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Technological characterization and survival of the exopolysaccharide-producing strain Lactobacillus delbrueckii subsp. lactis 193 and its bile-resistant derivative 193+ in simulated gastric and intestinal juices

Published online by Cambridge University Press:  21 July 2011

Patricia Burns ,
Gabriel Vinderola ,
Jorge Reinheimer ,
Isabel Cuesta ,
Clara G de los Reyes-Gavilán  and
Patricia Ruas-Madiedo
Patricia Burns
Affiliation:
Instituto de Productos Lácteos de Asturias-Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Departamento de Microbiología y Bioquímica de Productos Lácteos. Carretera de Infiesto s/n, 33300 Villaviciosa, Asturias, Spain Instituto de Lactología Industrial (INLAIN, UNL-CONICET), Facultad de Ingeniería Química, Universidad Nacional del Litoral, 1° de Mayo 3250, Santa Fe (3000), Argentina
Gabriel Vinderola
Affiliation:
Instituto de Lactología Industrial (INLAIN, UNL-CONICET), Facultad de Ingeniería Química, Universidad Nacional del Litoral, 1° de Mayo 3250, Santa Fe (3000), Argentina
Jorge Reinheimer
Affiliation:
Instituto de Lactología Industrial (INLAIN, UNL-CONICET), Facultad de Ingeniería Química, Universidad Nacional del Litoral, 1° de Mayo 3250, Santa Fe (3000), Argentina
Isabel Cuesta
Affiliation:
Instituto de Productos Lácteos de Asturias-Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Departamento de Microbiología y Bioquímica de Productos Lácteos. Carretera de Infiesto s/n, 33300 Villaviciosa, Asturias, Spain
Clara G de los Reyes-Gavilán*
Affiliation:
Instituto de Productos Lácteos de Asturias-Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Departamento de Microbiología y Bioquímica de Productos Lácteos. Carretera de Infiesto s/n, 33300 Villaviciosa, Asturias, Spain
Patricia Ruas-Madiedo
Affiliation:
Instituto de Productos Lácteos de Asturias-Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Departamento de Microbiología y Bioquímica de Productos Lácteos. Carretera de Infiesto s/n, 33300 Villaviciosa, Asturias, Spain
*
For correspondance; e-mail:[email protected]
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Abstract

The capacity of lactic acid bacteria to produce exopolysaccharides (EPS) conferring microorganisms a ropy phenotype could be an interesting feature from a technological point of view. Progressive adaptation to bile salts might render some lactobacilli able to overcome physiological gut barriers but could also modify functional properties of the strain, including the production of EPS. In this work some technological properties and the survival ability in simulated gastrointestinal conditions of Lactobacillus delbrueckii subsp. lactis 193, and Lb. delbrueckii subsp. lactis 193+, a strain with stable bile-resistant phenotype derived thereof, were characterized in milk in order to know whether the acquisition of resistance to bile could modify some characteristics of the microorganism. Both strains were able to grow and acidify milk similarly; however the production of ethanol increased at the expense of the aroma compound acetaldehyde in milk fermented by the strain 193+, with respect to milk fermented by the strain 193. Both microorganisms produced a heteropolysaccharide composed of glucose and galactose, and were able to increase the viscosity of fermented milks. In spite of the higher production yield of EPS by the bile-resistant strain 193+, it displayed a lower ability to increase viscosity than Lb. delbrueckii subsp. lactis 193. Milk increased survival in simulated gastric juice; the presence of bile improved adhesion to the intestinal cell line HT29-MTX in both strains. However, the acquisition of a stable resistance phenotype did not improve survival in simulated gastric and intestinal conditions or the adhesion to the intestinal cell line HT29-MTX. Thus, Lb. delbrueckii subsp. lactis 193 presents suitable technological properties for the manufacture of fermented dairy products; the acquisition of a stable bile-resistant phenotype modified some properties of the microorganism. This suggests that the possible use of bile-resistant derivative strains should be carefully evaluated in each specific application considering the influence that the acquisition of a stable bile-resistant phenotype could have in survival ability in gastric and intestinal conditions and in technological properties.

Keywords

exopolysaccharidesLactobacillus delbrueckii subsp. lactisbilegastric juiceintestinal juice
Type
Research Article
Information
Journal of Dairy Research , Volume 78 , Issue 3 , August 2011 , pp. 357 - 364
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

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