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Supplementing conjugated and non-conjugated L-methionine and acetate alters expression patterns of CSN2, proteins and metabolites related to protein synthesis in bovine mammary cells

Published online by Cambridge University Press:  02 March 2020

Seung-Woo Jeon ,
Jay Ronel Conejos ,
Jungeun Kim ,
Min-Jeong Kim ,
Jeong-Eun Lee ,
Baek-Seok Lee ,
Jin-Seung Park ,
Jun-Ok Moon ,
Jae-Sung Lee  and
Hong-Gu Lee
Seung-Woo Jeon
Affiliation:
Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea
Jay Ronel Conejos
Affiliation:
Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea
Jungeun Kim
Affiliation:
Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea
Min-Jeong Kim
Affiliation:
Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea
Jeong-Eun Lee
Affiliation:
CJ CheilJedang Research Institute of Biotechnology, Suwon, Republic of Korea
Baek-Seok Lee
Affiliation:
CJ CheilJedang Research Institute of Biotechnology, Suwon, Republic of Korea
Jin-Seung Park
Affiliation:
CJ CheilJedang Research Institute of Biotechnology, Suwon, Republic of Korea
Jun-Ok Moon
Affiliation:
CJ CheilJedang Research Institute of Biotechnology, Suwon, Republic of Korea
Jae-Sung Lee
Affiliation:
Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea
Hong-Gu Lee*
Affiliation:
Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea
*
Author for correspondence: Hong-Gu Lee, Email: [email protected]
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Abstract

The experiments reported in this research paper aimed to determine the effect of supplementing different forms of L-methionine (L-Met) and acetate on protein synthesis in immortalized bovine mammary epithelial cell line (MAC-T cells). Treatments were Control, L-Met, conjugated L-Met and acetate (CMA), and non-conjugated L-Met and Acetate (NMA). Protein synthesis mechanism was determined by omics method. NMA group had the highest protein content in the media and CSN2 mRNA expression levels (P < 0.05). The number of upregulated and downregulated proteins observed were 39 and 77 in L-Met group, 62 and 80 in CMA group and 50 and 81 in NMA group from 448 proteins, respectively (P < 0.05). L-Met, NMA and CMA treatments stimulated pathways related to protein and energy metabolism (P < 0.05). Metabolomic analysis also revealed that L-Met, CMA and NMA treatments resulted in increases of several metabolites (P < 0.05). In conclusion, NMA treatment increased protein concentration and expression level of CSN2 mRNA in MAC-T cells compared to control as well as L-Met and CMA treatments through increased expression of milk protein synthesis-related genes and production of the proteins and metabolites involved in energy and protein synthesis pathways.

Keywords

acetateL-methionineomicsprotein synthesis
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 1 , February 2020 , pp. 70 - 77
Copyright
Copyright © Hannah Dairy Research Foundation 2020

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Footnotes

*

Equal Contribution Status.

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